A5059367257- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Fungal and yeast genetics research
- Probiotics and Fermented Foods
- Pneumocystis jirovecii pneumonia detection and treatment
- Antimicrobial Peptides and Activities
- Bioinformatics and Genomic Networks
- Microbial Metabolic Engineering and Bioproduction
- Fungal Biology and Applications
- Peptidase Inhibition and Analysis
- Polyamine Metabolism and Applications
- Subtitles and Audiovisual Media
- Technology Adoption and User Behaviour
- Multi-Agent Systems and Negotiation
- Fermentation and Sensory Analysis
- Vector-borne infectious diseases
- Tactile and Sensory Interactions
- Microbial Metabolites in Food Biotechnology
- Genomics and Phylogenetic Studies
- Yeasts and Rust Fungi Studies
- Impact of Technology on Adolescents
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Service-Oriented Architecture and Web Services
- Gut microbiota and health
University of Lisbon
2015-2023
Institute for Biotechnology and Bioengineering
2015-2022
Instituto Superior Técnico
2015-2022
Instituto Politécnico de Lisboa
2015-2022
Iscte – Instituto Universitário de Lisboa
2008
Abstract The YEASTRACT+ information system (http://YEASTRACT-PLUS.org/) is a wide-scope tool for the analysis and prediction of transcription regulatory associations at gene genomic levels in yeasts biotechnological or human health relevance. new portal that integrates previously existing YEASTRACT (http://www.yeastract.com/) PathoYeastract (http://pathoyeastract.org/) databases introduces NCYeastract (Non-Conventional Yeastract) database (http://ncyeastract.org/), focused on so-called...
The YEAst Search for Transcriptional Regulators And Consensus Tracking (YEASTRACT-www.yeastract.com) information system has been, 11 years, a key tool the analysis and prediction of transcription regulatory associations at gene genomic levels in Saccharomyces cerevisiae. Since its last update June 2017, YEASTRACT includes approximately 163000 between factors (TF) target genes S. cerevisiae, based on more than 1600 bibliographic references; it also 247 specific DNA binding consensus...
Abstract YEASTRACT+ (http://yeastract-plus.org/) is a tool for the analysis, prediction and modelling of transcription regulatory data at gene genomic levels in yeasts. It incorporates three integrated databases: YEASTRACT (http://yeastract-plus.org/yeastract/), PathoYeastract (http://yeastract-plus.org/pathoyeastract/) NCYeastract (http://yeastract-plus.org/ncyeastract/), focused on Saccharomyces cerevisiae, pathogenic yeasts Candida genus, non-conventional biotechnological relevance. In...
Azoles are widely used antifungal drugs. This family of compounds includes triazoles, mostly in the treatment systemic infections, and imidazoles, such as clotrimazole, often case superficial infections. Candida glabrata is second most common cause candidemia worldwide presents higher levels intrinsic azole resistance when compared with albicans, thus being an interesting subject for study mechanisms fungal pathogens.Since relies on action membrane transporters, including drug efflux pumps...
5-Flucytosine is currently used as an antifungal drug in combination therapy, but fungal pathogens are rapidly able to develop resistance against this drug, compromising its therapeutic action. The understanding of the underlying mechanisms crucial deal with problem. In work, S. cerevisiae deletion mutant collection was screened for increased flucytosine. Through chemogenomics analysis, 183 genes were found confer agent. Consistent known effect DNA, RNA and protein synthesis, most...
We present the PATHOgenic YEAst Search for Transcriptional Regulators And Consensus Tracking (PathoYeastract - http://pathoyeastract.org) database, a tool analysis and prediction of transcription regulatory associations at gene genomic levels in pathogenic yeasts Candida albicans C. glabrata. Upon data retrieval from hundreds publications, followed by curation, database currently includes 28 000 unique documented between factors (TF) target genes 107 DNA binding sites, considering 134 TFs...
Candida glabrata is an emerging fungal pathogen. Its increased prevalence associated with its ability to rapidly develop antifungal drug resistance, particularly azoles. In order unravel new molecular mechanisms behind azole a transcriptomics analysis of the evolution C. clinical isolate (isolate 044) from susceptibility posaconazole resistance (21st day), clotrimazole (31st and fluconazole voriconazole (45th induced by longstanding incubation fluconazole, was carried out. All evolved...
The mechanisms of persistence and virulence associated with Candida glabrata infections are poorly understood, limiting the ability to fight this fungal pathogen. In study, multidrug resistance transporters CgTpo1_1 CgTpo1_2 shown play a role in C. virulence. survival infection model Galleria mellonella, infected glabrata, was found increase upon deletion either CgTPO1_1 or CgTPO1_2. underlying were further explored. case CgTpo1_1, phenotype be consistent observation that it confers...
Resistance to 5-flucytosine (5-FC), used as an antifungal drug in combination therapy, compromises its therapeutic action. In this work, the response of human pathogen Candida glabrata 5-FC was evaluated at membrane proteome level, using iTRAQ-based approach. A total 32 proteins were found display significant expression changes fraction cells upon exposure 5-FC, 50% which under control CgPdr1, major regulator azole resistance. These cluster into functional groups associated cell wall...
The ability to acquire azole resistance is an emblematic trait of the fungal pathogen
The emergence of drug resistance significantly hampers the treatment human infections, including those caused by fungal pathogens such as Candida species. glabrata ranks second most common cause candidiasis worldwide, supported rapid acquisition to azole and echinocandin antifungals frequently prompted single nucleotide polymorphisms (SNPs) in associated genes, PDR1 (azole resistance) or FKS1/2 (echinocandin resistance). To determine frequency genome rearrangements possible genetic basis C....
Candida glabrata is an opportunistic pathogen that adheres to human epithelial mucosa and forms biofilm cause persistent infections. In this work, Single-cell Force Spectroscopy (SCFS) was used glimpse at the adhesive properties of C. as it interacts with clinically relevant surfaces, first step towards formation. Following a genetic screening, RNA-sequencing revealed half entire transcriptome remodeled upon formation, around 40% which under control transcription factors CgEfg1 CgTec1. Using...
Persistence and virulence of Candida glabrata infections are multifactorial phenomena, whose understanding is crucial to design more suitable therapeutic strategies. In this study, the putative multidrug transporter CgDtr1, encoded by ORF CAGL0M06281g, identified as a determinant C. in infection model Galleria mellonella. CgDTR1 deletion shown decrease ability kill G. mellonella larvae decreasing proliferate hemolymph, tolerate action hemocytes. The possible role CgDtr1 resistance several...
The prevalence of antifungal resistance in Candida glabrata, especially against azole drugs, results difficult-to-treat and potentially life-threatening infections. Understanding the molecular basis C. glabrata is crucial to designing more suitable therapeutic strategies. In this study, role transcription factor encoded by ORF CAGL0B03421g, here denominated as CgMar1 (Multiple Azole Resistance 1), susceptibility was explored. Using RNA-sequencing, found regulate 337 genes under fluconazole...
Candida glabrata is a prominent pathogenic yeast which exhibits unique ability to survive the harsh environment of host immune cells. In this study, we describe role transcription factor encoded by gene CAGL0F09229g, here named CgTog1 after its Saccharomyces cerevisiae ortholog, as new determinant C. virulence. Interestingly, Tog1 absent in other clinically relevant species (C. albicans, parapsilosis, tropicalis, auris), being exclusive glabrata. was found be required for oxidative stress...
Candida glabrata is an emerging fungal pathogen whose success depends on its ability to resist antifungal drugs but also thrive against host defenses. In this study, the predicted multidrug transporter CgTpo4 (encoded by ORF CAGL0L10912g) described as a new determinant of virulence in C. glabrata, using infection model Galleria mellonella. The CgTPO4 gene was found be required for kill G. encoded necessary antimicrobial peptide (AMP) resistance, specifically histatin-5. Interestingly,...
The increasing prevalence of candidosis caused by Candida glabrata is related to its ability acquire azole resistance. Although resistance mechanisms are well known, the for import into fungal cells have remained obscure. In this work, we characterized two hexose transporters in C. and further investigate their role as potential importers. Three susceptible clinical isolates were evolved towards acquired phenotype was found be independent CgPDR1 or CgERG11 mutations. Through whole-genome...
Here, we report the draft genome sequences of three Candida glabrata clinical isolates, 040, 044, and OL152. The isolates were recovered from patients admitted to Centro Hospitalar de S. João (CHSJ) in Porto, Portugal. Isolates 040 044 taken blood samples, while isolate OL152 was collected urine.