A5050741197- Marine Ecology and Invasive Species
- Developmental Biology and Gene Regulation
- Echinoderm biology and ecology
- Marine Biology and Environmental Chemistry
- Neurobiology and Insect Physiology Research
- Marine Bivalve and Aquaculture Studies
- Cephalopods and Marine Biology
- Hemiptera Insect Studies
- Animal Genetics and Reproduction
- Parasite Biology and Host Interactions
- Marine Invertebrate Physiology and Ecology
- Planarian Biology and Electrostimulation
- Hippo pathway signaling and YAP/TAZ
- Action Observation and Synchronization
- Social Robot Interaction and HRI
- Retinal Development and Disorders
- Insect and Arachnid Ecology and Behavior
- Marine Biology and Ecology Research
- Wnt/β-catenin signaling in development and cancer
- Marine and coastal plant biology
- Beetle Biology and Toxicology Studies
- Insect Pheromone Research and Control
- Human Motion and Animation
- Cellular Mechanics and Interactions
- Cancer-related gene regulation
Hiroshima University
2013-2023
Universitas Islam Negeri Maulana Malik Ibrahim
2023
Marine Biological Laboratory
2010-2015
The University of Texas at Austin
2008
University of Houston
2006
University of Hawaii System
2000-2005
University of Hawaiʻi at Mānoa
2004
The University of Texas Health Science Center at Houston
2004
Kyoto University
1996-2002
Acorn worms, also known as enteropneust (literally, 'gut-breathing') hemichordates, are marine invertebrates that share features with echinoderms and chordates. Together, these three phyla comprise the deuterostomes. Here we report draft genome sequences of two acorn Saccoglossus kowalevskii Ptychodera flava. By comparing them diverse bilaterian genomes, identify shared traits were probably inherited from last common deuterostome ancestor, then explore evolutionary trajectories leading this...
ABSTRACT This work concerns the formation of mesoderm in development an enteropneust hemichordate, Ptychodera flava, and expression Brachyury gene during this process. occurs two distinct phases. In embryo, is transcribed gastrulation future oral anal regions gut, but transcripts are no longer detected by 2 weeks development. not 5 months larval planktonic existence. During time, adult coeloms begin to develop, originating as coalescences cells that appear delaminate from wall gut. cannot be...
SUMMARY Using the Hawaiian acorn worm, Ptychodera flava , we began molecular studies on development of hemichordates, a phylum previously unstudied at this level. Here review results garnered from examination few specific genes selected to help understand evolution vertebrate structures. These suggest new ideas about developmental mechanisms in deuterostomes. In seminal observation, noted an unexpected zone expression Brachyury gene early anterior embryonic ectoderm where mouth will form....
We have identified and characterized the sequence expression of two Group B Sox genes in acorn worm, Ptychodera flava. One represents a B1 gene is designated Pf-SoxB1; other B2 Pf-SoxB2. Both encode polypeptides with an HMG domain N-terminal half. Whole-mount situ hybridization to embryonic larval stages P. flava shows that are expressed rather similar patterns at these stages. Expression first detected cells blastula subsequently localizes ectoderm during gastrulation. As mouth forms,...
Abstract Background ParaHox and Hox genes are thought to have evolved from a common ancestral ProtoHox cluster or tandem duplication prior the divergence of cnidarians bilaterians. Similar clusters, chordate including Gsx , Xlox Cdx clustered their expression exhibits temporal spatial colinearity. In non-chordate animals, however, studies on genomic organization limited only few animal taxa. Hemichordates, such as Enteropneust acorn worms, been used gain insights into origins characters....
T-box genes encode a novel family of sequence-specific activators that appear to play crucial roles in various processes animal development. Although most the are involved mesoderm formation chordate embryos, mammalian T-Brain is expressed developing central nervous system, and defines molecularly distinct domains within cerebral cortex. Here we report first invertebrate homologue from hemichordate acorn worm, Ptychodera flava, which designate Pf-Tbrain. Developmental expression Pf-Tbrain...
The auricularia larva of sea cucumbers and tornaria acorn worms share striking developmental morphological similarities. They are regarded as not only an archetype the nonchor-date deuterostome larva, but also origin chordates. Here we report characterization spatial expression patterns BMP-2/4 genes a hemichordate worm (Pf-bmp2/4) echinoderm cucumber (Sj-bmp2/4). Both Pf-bmp2/4 Sj-bmp2/4 exhibited apparently conserved in region coelomopore complex. This is agreement with homology between...
We report scanning and transmission electron microscopic studies of the early development Hawaiian acorn worm, Ptychodera flava. In addition, we provide an immunohistochemical identification larval nervous system. Development occurs is constrained within stout chorion fertilization envelope that forms upon release cortical granules in cytoplasm egg. The blastula consists tall columnar blastomeres encircling a small blastocoel. Typical gastrulation definitive tornaria formed compressed...
SUMMARY Although data are available from only vertebrates, urochordates, and three nonchordate animals, there definite differences in the structures of classic cadherins between vertebrates plus urochordates nonchordates. In this study we examined structural diversity among bilaterian animals by obtaining new an amphioxus (Cephalochordata, Chordata), acorn worm (Hemichordata), a sea star (Echinodermata), oyster (Mollusca). The newly identified grouped together with those known urchin...
The spawning and early embryogenesis of the hemichordate, Ptychodera flava, in Hawaii are described detail illustrated with photographs living material. Natural evenings December was induced by a shift seawater temperature from about 22 degrees C to 26 C. fertilized egg divides equally slowly at first, reaching 8 cells 5 hr after insemination room (20-24 C). Divisions then appear become slightly unequal 9 embryo has divided into 100 cells. blastocoel forms during cleavage as an irregular...
When the body of P. flava is severed, animal has ability to regenerate its missing anterior or posterior as appropriate. We have focused on regeneration when head and branchial regions are severed from worm. After transection, wall contracts heals closed in 2 3 days. By third day a small blastema evident at point closure. The grows rapidly begins process differentiating into with proboscis collar. At 5 days increased greatly size differentiated central bulb, forming proboscis, two lateral...
Abstract Background Acoels are primitive bilaterians with very simple soft bodies, in which many organs, including the gut, not developed. They provide platforms for studying molecular and developmental mechanisms involved formation of basic bilaterian body plan, whole-body regeneration, symbiosis photosynthetic microalgae. Because genomic information is essential future research on acoel biology, we sequenced assembled nuclear genome an acoel, Praesagittifera naikaiensis. Findings To avoid...
SUMMARY Molecular and morphological comparisons indicate that the Echinodermata Hemichordata represent closely related sister‐phyla within Deuterostomia. Much less is known about development of hemichordates compared to other deuterostomes. For first time, cell lineage analyses have been carried out for an indirect‐developing representative enteropneust hemichordates, Pty‐ chodera flava . Single blastomeres were iontophoretically labeled with DiI at 2‐ through 16‐cell stages, their fates...
The adherens junction (AJ) is the most universal found in bilaterian epithelia and may represent one of earliest types cell-cell junctions. adhesion molecules responsible for forming AJs are classic cadherins (referred to simply as cadherins), whose extracellular domain organization displays marked variety among species examined so far. In this study, we attempted reconstruct evolution cadherin by analyzing new data from several arthropods (two insects, non-insect hexapod, three crustaceans,...
Hemichordates occupy a critical phylogenetic position among deuterostomes because they exhibit echinoderm-like larval morphology and chordate-like adult morphology. Analyses of the expression function hemichordate developmental genes will therefore provide insight into evolution deuterostome body plans. The distal-less/dlx gene encodes homeodomain transcription factor plays roles in development appendages brain variety animals. Here we have characterized distal-less (Pf-dlx) Ptychodera...
Hedgehog is a toolkit gene conserved in metazoans. However, its function differs among taxa, and it shows versatile expression patterns morphogenesis. We analyzed the pattern of hedgehog indirect development hemichordate, Ptychodera flava, during regeneration. Pf-Hh showed distinct enteropneust-specific at anterior tip larvae, as well deuterostome-conserved pharyngeal endoderm. In contrast, expressed only region regeneration, but not proboscis. These data suggest that regeneration driven by...