A5069513401- Developmental Biology and Gene Regulation
- Spider Taxonomy and Behavior Studies
- Insect Resistance and Genetics
- Subterranean biodiversity and taxonomy
- Neurobiology and Insect Physiology Research
- Wnt/β-catenin signaling in development and cancer
- Hedgehog Signaling Pathway Studies
- Genomics and Phylogenetic Studies
- Marine Ecology and Invasive Species
- Genomics and Chromatin Dynamics
- Insect and Arachnid Ecology and Behavior
- Invertebrate Immune Response Mechanisms
- Cellular Mechanics and Interactions
- Environmental DNA in Biodiversity Studies
- Silk-based biomaterials and applications
- Genetics, Aging, and Longevity in Model Organisms
- Marine Biology and Environmental Chemistry
- Plant Molecular Biology Research
- Venomous Animal Envenomation and Studies
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Hippo pathway signaling and YAP/TAZ
- Beetle Biology and Toxicology Studies
- Neurogenesis and neuroplasticity mechanisms
- Genomic variations and chromosomal abnormalities
Osaka Science Museum
2008-2022
Japan Science and Technology Agency
2004-2022
Osaka University of Pharmaceutical Sciences
2022
Japan Tobacco (Japan)
2022
Osaka Medical and Pharmaceutical University
2016-2020
The University of Tokyo
1998-2003
Kyoto University
2000
The duplication of genes can occur through various mechanisms and is thought to make a major contribution the evolutionary diversification organisms. There increasing evidence for large-scale in some chelicerate lineages including two rounds whole genome (WGD) horseshoe crabs. To investigate this further, we sequenced analyzed common house spider Parasteatoda tepidariorum. We found pervasive both coding non-coding spider, clusters Hox genes. Analysis synteny conservation across P....
In early embryogenesis of spiders, the cumulus is characteristically observed as a cellular thickening that arises from center germ disc and moves centrifugally. This movement breaks radial symmetry morphology, correlating with development dorsal region embryo. Classical experiments on spider embryos have shown has capacity to induce secondary axis when transplanted ectopically. this study, we examined house spider, Achaearanea tepidariorum, basis knowledge Drosophila characterize at...
The mechanism by which Decapentaplegic (Dpp) and its antagonist Short gastrulation (Sog) specify the dorsoventral pattern in Drosophilaembryos has been proposed to have a common origin with that organizes body axis vertebrate embryo. However, DrosophilaSog makes only minor contributions development of ventral structures hypothetically correspond dorsum where axial notochord forms. In this study, we isolated homologue Drosophila sog gene spider Achaearanea tepidariorum, characterized...
Homeobox genes are key toolkit that regulate the development of metazoans and changes in their regulation copy number have contributed to evolution phenotypic diversity. We recently identified a whole genome duplication (WGD) event occurred an ancestor spiders scorpions (Arachnopulmonata), many homeobox genes, including two Hox clusters, appear been retained arachnopulmonates. To better understand consequences this ancient WGD arachnid we characterized compared repertoires range arachnids....
In the development of most arthropods, caudal region elongating germ band (the growth zone) sequentially produces new segments. Previous work with spider Cupiennius salei suggested involvement Delta-Notch signaling in segmentation. Here, we report that, Achaearanea tepidariorum, same pathway exerts a different function presumptive before initiation developing embryo, zone becomes morphologically apparent as lobe around closed blastopore. We found preceding formation, transcripts Delta...
During development segmentation is a process that generates spatial periodic pattern. Peak splitting of waves gene expression mathematically predicted, simple strategy accounting for this type process, but it has not been well characterized biologically. Here we show temporally repeated into stripes associated with head axis growth in the spider Achaearanea embryo. Preceding segmentation, wave hedgehog homologue observed to travel posteriorly during stage 6. This stripe, co-expressing an...
The early embryo of the spider Achaearanea tepidariorum is emerging as a model for simultaneous study cell migration and pattern formation. A cluster internalized at center radially symmetric germ disc expresses evolutionarily conserved dorsal signal Decapentaplegic. This migrates away from along basal side epithelium to rim. thought be symmetry-breaking event that establishes orientation dorsoventral axis. In this study, knockdown patched homolog, At-ptc, encodes putative negative regulator...
In the development of Drosophila central nervous system, some neuroblasts designated as neuroglioblasts generate both glia and neurons. Little is known about how produce these different cell types. NB6-4 in thoracic segment (NB6-4T) a neuroglioblast, although corresponding abdominal (NB6-4A) produces only glia. Here, we describe divisions NB6-4T lineage, following changes number arrangement. We also examined successive expression glial cells missing (gcm) mRNA protein, activity which to...
We cloned an Achaearanea tepidariorum (Chelicerata, Arachnida) gene related to Drosophila twist (twi), which encodes a basic helix-loop-helix transcription factor required specify mesoderm fate in the embryo. The spider was designated At.twist (At.twi). examined its expression by whole-mount situ hybridization. At.twi transcripts were first detected cells located at polar and equatorial areas of spherical embryo when cumulus reached equator. As extra-embryonic area expanded, more expressed...
The simultaneous and quantitative analysis of the expression multiple genes helps to shed light on gene regulatory networks. We established a method for multi-color fluorescence in situ hybridization (mFISH) cell-type diversification developmental regulation embryo spider Parasteatoda tepidariorum. This mFISH technique allowed quadruple staining using four types labels RNA probes, digoxigenin, fluorescein, biotin, dinitrophenyl, together with different fluorescent tyramides. To validate...
SUMMARY Although data are available from only vertebrates, urochordates, and three nonchordate animals, there definite differences in the structures of classic cadherins between vertebrates plus urochordates nonchordates. In this study we examined structural diversity among bilaterian animals by obtaining new an amphioxus (Cephalochordata, Chordata), acorn worm (Hemichordata), a sea star (Echinodermata), oyster (Mollusca). The newly identified grouped together with those known urchin...
Classical cadherins are a metazoan-specific family of homophilic cell-cell adhesion molecules that regulate morphogenesis. Type I and type IV in this function at adherens junctions the major epithelial tissues vertebrates insects, respectively, but they have distinct, relatively simple domain organizations thought to evolved by independent reductive changes from an ancestral III cadherin, which is larger than derived paralogs has complicated organization. Although both been identified...
Hedgehog (Hh) signaling plays fundamental roles in animal body patterning. Understanding its mechanistic complexity requires simple tractable systems that can be used for these studies. In the early spider embryo, Hh mediates formation of overall anterior-posterior polarity, yet it remains unclear what mechanisms link initial activity with axis segmentation, which distinct periodic stripe-forming dynamics occur depending on region. We performed genome-wide searches genes transcriptionally...
Patterning along an axis of polarity is a fundamental step in the development multicellular animal embryo. In cellular field early spider embryo, Hedgehog signaling operates to specify "fuzzy" French-flag-like pattern primary axis, which related future anterior-posterior (A-P) axis. However, details regarding generation and diversity cell states based on embryo are not known. To address this issue, we applied single-cell RNA sequencing consisting approximately 2,000 cells. Our results...
The adherens junction (AJ) is the most universal found in bilaterian epithelia and may represent one of earliest types cell-cell junctions. adhesion molecules responsible for forming AJs are classic cadherins (referred to simply as cadherins), whose extracellular domain organization displays marked variety among species examined so far. In this study, we attempted reconstruct evolution cadherin by analyzing new data from several arthropods (two insects, non-insect hexapod, three crustaceans,...
One of the conserved traits arthropod embryonic development is striped expression homologs Drosophila segment polarity genes, including hedgehog (hh). Although a diversity stripe-forming processes recognized among embryos, such varied have not been well characterized from cellular and quantitative perspectives. The spider Parasteatoda tepidariorum embryo, which has hh-dependent mechanism axis formation, offers cell-based field where stripes Pt-hh (a hh homolog) dynamically develop in...
Abstract Background The process of early development varies across the species-rich phylum Arthropoda. Owing to limited research strategies for dissecting lineage-specific processes in arthropods, little is known about variations arthropod at molecular resolution. Theridiidae spider, Parasteatoda tepidariorum , has its genome sequenced and could potentially contribute embryonic processes. Results We present genome-wide identification candidate genes that exhibit locally restricted expression...
We previously reported the existence of Bb-cadherin, a molecule related to classic cadherin, in cephalochordate amphioxus (Branchiostoma belcheri). The structure Bb-cadherin is unique that it lacks cadherin extracellular repeats, although its cytoplasmic domain shows close similarities those typical cadherins. region consists laminin globular domains and cysteine-rich EGF-like are similar nonchordate In this study, we identified second cadherin. It was designated Bb2-cadherin (Bb2C) while...
ABSTRACT During development of the Drosophila central nervous system, neuroblast 6-4 in thoracic segment (NB6-4T) divides asymmetrically into a medially located glial precursor cell and laterally neuronal cell. In this study, to understand molecular basis for glia-neuron cell-fate decision, we examined effects some known mutations on NB6-4T lineage. First, found that prospero (pros) led loss expression Glial cells missing, which is essential trigger differentiation, wild-type embryos, Pros...
Abstract The duplication of genes can occur through various mechanisms and is thought to make a major contribution the evolutionary diversification organisms. There increasing evidence for large-scale in some chelicerate lineages including two rounds whole genome (WGD) horseshoe crabs. To investigate this further we sequenced analyzed common house spider Parasteatoda tepidariorum. We found pervasive both coding non-coding spider, clusters Hox genes. Analysis synteny conservation across P....