A5064601858- Genetic and Kidney Cyst Diseases
- Hedgehog Signaling Pathway Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Developmental Biology and Gene Regulation
- Neurobiology and Insect Physiology Research
- Animal Genetics and Reproduction
- Genomics and Phylogenetic Studies
- Crustacean biology and ecology
- Renal and related cancers
- Genomics and Chromatin Dynamics
- Venomous Animal Envenomation and Studies
- Fetal and Pediatric Neurological Disorders
- Silkworms and Sericulture Research
- Invertebrate Immune Response Mechanisms
- Subterranean biodiversity and taxonomy
- interferon and immune responses
- Marine Biology and Environmental Chemistry
- Epigenetics and DNA Methylation
- Pancreatic function and diabetes
- Microbial Metabolic Engineering and Bioproduction
- Genetic Syndromes and Imprinting
- Mitochondrial Function and Pathology
- Protist diversity and phylogeny
- Aquaculture Nutrition and Growth
- ATP Synthase and ATPases Research
Cincinnati Children's Hospital Medical Center
2020-2025
University of Cincinnati Medical Center
2022-2024
Citigroup
2024
Universidade Federal de Santa Catarina
2016-2022
Oxford Brookes University
2016-2018
The duplication of genes can occur through various mechanisms and is thought to make a major contribution the evolutionary diversification organisms. There increasing evidence for large-scale in some chelicerate lineages including two rounds whole genome (WGD) horseshoe crabs. To investigate this further, we sequenced analyzed common house spider Parasteatoda tepidariorum. We found pervasive both coding non-coding spider, clusters Hox genes. Analysis synteny conservation across P....
Bacterial artificial chromosome transgenic models, including most Cre-recombinases, enable potent interrogation of gene function in vivo but require rigorous validation as limitations emerge. Due to its high relevance metabolic studies, we perform comprehensive analysis the Ucp1-CreEvdr line which is widely used for brown fat research. Hemizygotes exhibit major and white transcriptomic dysregulation, indicating potential altered tissue function. homozygotes also show mortality, specific...
In short germ arthropods, posterior segments are added sequentially from a growth zone or segment addition (SAZ) during embryogenesis. Studies in spiders such as the common house spider, Parasteatoda tepidariorum, have provided insights into gene regulatory network (GRN) that underlies development of SAZ, and revealed involvement two important signalling pathways. It was shown Wnt8 maintains pool undifferentiated cells but this ligand is also required for dynamic Delta (Dl) expression...
Sox genes encode a set of highly conserved transcription factors that regulate many developmental processes. In insects, the SoxB gene Dichaete is only known to be involved in segmentation. To determine if similar mechanisms are used other arthropods, we investigated role during segmentation spider Parasteatoda tepidariorum. While does not appear segmentation, found closely related Sox21b-1 acts as gap formation anterior segments and also part clock for development segment addition zone...
Primary cilia are nearly ubiquitous organelles that transduce molecular and mechanical signals. Although the basic structure of cilium cadre genes contribute to ciliary formation function (the ciliome) believed be evolutionarily conserved, presentation ciliopathies with narrow, tissue-specific phenotypes distinct readouts suggests an unappreciated heterogeneity exists within this organelle. Here, we provide a searchable transcriptomic resource for curated primary ciliome, detailing various...
The Sox family of transcription factors is an important part the genetic 'toolbox' all metazoans examined to date and known play developmental roles in vertebrates insects. However, outside commonly studied Drosophila model little about repertoire other arthropod species. Here we characterise two chelicerate species, spiders Parasteatoda tepidariorum Stegodyphus mimosarum, which have experienced a whole genome duplication (WGD) their evolutionary history. We find that virtually duplicate...
Abstract Hox genes encode transcription factors that specify the body segment identity during development, including crustaceans, such as amphipods and decapods, possess a remarkable diversity of segments specialized appendages. In amphipods, alterations appendages have been obtained using knockout experiment genes, which suggests these are involved in evolution morphology within crustaceans. However, studies crustaceans limited to few species. Here, we identified homeodomain nine genes:...
Abstract The duplication of genes can occur through various mechanisms and is thought to make a major contribution the evolutionary diversification organisms. There increasing evidence for large-scale in some chelicerate lineages including two rounds whole genome (WGD) horseshoe crabs. To investigate this further we sequenced analyzed common house spider Parasteatoda tepidariorum. We found pervasive both coding non-coding spider, clusters Hox genes. Analysis synteny conservation across P....
Ciliopathies represent a growing class of diseases caused by defects in microtubule-based organelles called primary cilia. Approximately 30% ciliopathies are characterized craniofacial phenotypes such as craniosynostosis, cleft lip/palate and micrognathia. Patients with ciliopathic micrognathia experience particular set difficulties, including impaired feeding breathing, have extremely limited treatment options. To understand the cellular molecular basis for micrognathia, we used talpid2...
Abstract Background The Sox family of transcription factors are present and conserved in the genomes all metazoans examined to data known play important developmental roles vertebrates insects. However, outside commonly studied Drosophila model little is about extent or conservation other arthropod species. Here we characterise two chelicerate species, spiders Parasteatoda tepidariorum Stegodyphus mimosarum , which have experienced a whole genome duplication (WGD) their evolutionary history....
The naturally occurring chicken mutant talpid2 (ta2 ), best known for its limb and craniofacial defects, has long served as a valuable tool developmental biologists studying growth patterning of structures the limb. provides unique to examine molecular cellular processes regulating development.This also insights into evolution genetic programs. Previous work defined appearance atavistic dentition in ta2 embryos. Herein we describe ancestral characters hindlimb embryonic embryos.As phenotype...
Primary cilia are ubiquitous microtubule-based organelles that serve as signaling hubs for numerous developmental pathways, most notably the Hedgehog (Hh) pathway. Defects in structure or function of primary result a class diseases called ciliopathies. It is well known participate transducing Hh signal, and such ciliopathies frequently present with phenotypes indicative aberrant function. Interestingly, exact mechanisms cilia-dependent transduction unclear some ciliopathic animal models...
Ciliopathies represent a disease class characterized by broad range of phenotypes including polycystic kidneys and skeletal anomalies. Ciliopathic are among the most common difficult to treat due poor understanding pathological mechanisms leading disease. Using an avian model (talpid2) for human ciliopathy with both kidney anomalies (orofaciodigital syndrome 14), we identified disruptions in FGF23-PTH axis that resulted reduced calcium uptake developing mandible subsequent micrognathia....
Abstract The mandible is composed of several musculoskeletal tissues including bone, cartilage, and tendon that require precise patterning to ensure structural functional integrity. Interestingly, most these are derived from one multipotent cell population called cranial neural crest cells (CNCCs). How CNCCs properly instructed differentiate into various tissue types remains nebulous. To better understand the mechanisms necessary for mandibular we utilized avian mutant talpid 2 ( ta ) which...
The mandible is composed of several musculoskeletal tissues including bone, cartilage, and tendon that require precise patterning to ensure structural functional integrity. Interestingly, most these are derived from one multipotent cell population called cranial neural crest cells (CNCCs). How CNCCs properly instructed differentiate into various tissue types remains nebulous. To better understand the mechanisms necessary for mandibular we utilized avian mutant talpid
Summary Bacterial artificial chromosome transgenic models, including most Cre-recombinases , enable potent interrogation of gene function in vivo but require rigorous validation as limitations emerge. Due to its high relevance metabolic studies, we performed comprehensive analysis the Ucp1-Cre Evdr line which is widely used for brown fat research. Hemizygotes exhibited major and white transcriptomic dysregulation, indicating potential altered tissue function. homozygotes also show mortality,...
Summary The Sox gene family encode a set of highly conserved HMG domain transcription factors that regulate many key processes during metazoan embryogenesis. In insects, the SoxB Dichaete is only known to be involved in embryonic segmentation. To determine if similar mechanisms are used other arthropods, we investigated role genes segmentation spider Parasteatoda tepidariorum . While does not appear segmentation, RNAi knockdown closely related Sox21b-1 results gap like phenotype developing...
Abstract Ciliopathies represent a growing class of diseases caused by defects in microtubule-based organelles called primary cilia. Approximately 30% ciliopathies can be characterized craniofacial phenotypes such as craniosynostosis, cleft lip/palate and micrognathia. Patients with ciliopathic micrognathia experience particular set difficulties including impaired feeding breathing have extremely limited treatment options. To understand the cellular molecular basis for micrognathia, we...
Abstract Ciliopathies represent a disease class characterized by broad range of phenotypes including polycystic kidneys and skeletal anomalies. Ciliopathic are among the most common difficult to treat due poor understanding pathological mechanisms leading disease. Using an avian model ( talpid 2 ) for human ciliopathy with anomalies (Orofaciodigital syndrome 14), we identified disruptions in FGF23-PTH axis that resulted reduced calcium uptake developing mandible subsequent micrognathia....