A5059782499- Yersinia bacterium, plague, ectoparasites research
- Bacillus and Francisella bacterial research
- Vector-borne infectious diseases
- Vibrio bacteria research studies
- Plant-based Medicinal Research
- Bacterial Identification and Susceptibility Testing
- Bacteriophages and microbial interactions
- COVID-19 Clinical Research Studies
- Pharmacological Effects of Natural Compounds
- SARS-CoV-2 and COVID-19 Research
- Ethnobotanical and Medicinal Plants Studies
- Zoonotic diseases and public health
- Bee Products Chemical Analysis
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Burkholderia infections and melioidosis
- Long-Term Effects of COVID-19
- Animal Virus Infections Studies
- Microbial Metabolic Engineering and Bioproduction
- Chemical Looping and Thermochemical Processes
- Essential Oils and Antimicrobial Activity
- Zebrafish Biomedical Research Applications
- Inhalation and Respiratory Drug Delivery
- Probiotics and Fermented Foods
- Animal Disease Management and Epidemiology
Israel Institute for Biological Research
2015-2024
University of Louisville
2013-2014
Abstract The COVID-19 pandemic caused by SARS-CoV-2 imposes an urgent need for rapid development of efficient and cost-effective vaccine, suitable mass immunization. Here, we show the a replication competent recombinant VSV-∆G-spike in which glycoprotein VSV is replaced spike protein SARS-CoV-2. In-vitro characterization this vaccine indicates expression presentation on viral membrane with antigenic similarity to A golden Syrian hamster in-vivo model implemented. We that single-dose...
Messenger RNA (mRNA) lipid nanoparticle (LNP) vaccines have emerged as an effective vaccination strategy. Although currently applied toward viral pathogens, data concerning the platform's effectiveness against bacterial pathogens are limited. Here, we developed mRNA-LNP vaccine a lethal pathogen by optimizing mRNA payload guanine and cytosine content antigen design. We designed nucleoside-modified based on F1 capsule antigen, major protective component of Yersinia pestis, etiological agent...
ABSTRACT In a search for novel attenuated vaccine candidates use against Yersinia pestis , the causative agent of plague, signature-tagged mutagenesis strategy was used and optimized subcutaneously infected mouse model. A library tagged mutants virulent Y. Kimberley53 strain generated. Screening 300 through two consecutive cycles resulted in selection 16 mutant strains that were undetectable spleens 48 h postinfection. Each these evaluated vivo by assays competition wild-type virulence...
Yersinia pestis is the causative agent of plague. Previously we have isolated an attenuated Y. transposon insertion mutant in which pcm gene was disrupted. In present study, investigated expression and role locus genes pathogenesis using a set isogenic surE, pcm, nlpD rpoS mutants fully virulent Kimberley53 strain. We show that pestis, controlled from elements residing within upstream surE pcm. The NlpD lipoprotein only factor encoded essential for virulence. A chromosomal deletion sequence...
The enteropathogenic Yersinia strains are known to downregulate signaling pathways in macrophages by effectors of the type III secretion system, which YopJ/YopP plays a crucial role. adverse effects pestis, causative agent plague, were examined infecting J774A.1 cells, RAW264.7 and primary murine with EV76 strain fully virulent Kimberley53 strain. Y. pestis exerts YopJ-dependent suppression tumor necrosis factor alpha phosphorylation mitogen-activated protein kinases thus resembles Yersinia....
Pneumonic plague is a fatal disease caused by Yersinia pestis that associated with delayed immune response in the lungs. Because neutrophils are first cells recruited to sites of infection, we investigated mechanisms responsible for their homing lung. During 24 hr after pulmonary infection fully virulent Y. strain, no significant changes were observed lungs levels infiltrate, expression adhesion molecules, or major neutrophil chemoattractants keratinocyte cell-derived chemokine (KC),...
An important virulence strategy evolved by bacterial pathogens to overcome host defenses is the modulation of cell death. Previous observations have indicated that Yersinia pestis, causative agent plague disease, exhibits restricted capacity induce death in macrophages due ineffective translocation type III secretion effector YopJ, as opposed readily translocated YopP, YopJ homologue enteropathogen enterocolitica Oratio8. This led us suggest reduced cytotoxic potency may allow pathogen...
In a recent study, we demonstrated that vaccination with the polymeric F1 capsule antigen of plague pathogen Yersinia pestis led to rapid induction protective humoral immune response via pivotal activation innate-like B1b cells. Conversely, monomeric version failed promptly protect vaccinated animals in this model bubonic plague. examined ability confer onset immunity more challenging mouse pneumonic Vaccination one dose adsorbed on aluminum hydroxide elicited effective protection against...
Abstract The COVID-19 pandemic caused by SARS-CoV-2 that emerged in December 2019 China resulted over 7.8 million infections and 430,000 deaths worldwide, imposing an urgent need for rapid development of efficient cost-effective vaccine, suitable mass immunization. Here, we generated a replication competent recombinant VSV-ΔG-spike which the glycoprotein VSV was replaced spike protein SARS-CoV-2. In vitro characterization indicated expression presentation on viral membrane with antigenic...
The COVID-19 pandemic caused by the SARS-CoV-2 infection induced lung inflammation characterized cytokine storm and fulminant immune response of both resident migrated cells, accelerating alveolar damage. In this work we identified members matrix metalloprotease (MMPs) family associated with extra-cellular (ECM) destruction using K18-hACE2-transgenic mice (K18-hACE2) infected intranasally SARS-CoV-2. Five days post infection, lungs exhibited overall damage epithelial cells massive leukocytes...
Existing media designed for selective isolation of clinically important members the genus Yersinia were found to be unsatisfactory growth and pestis. We report development a new agar medium (termed BIN) that supports Y. The formulation this was based on fluorescence screening system monitoring bacterial semisolid media, using green fluorescent protein-expressing strain. High-throughput combinatorial experiments can conducted quantitative evaluation effect different components growth....
The encounter between invading microorganisms and dendritic cells (DC) triggers a series of events which include uptake degradation the microorganism, induction maturation process, enhancement DC migration to draining lymph nodes. Various pathogens have developed strategies counteract these as measure evade host defense. In present study we found that interaction Yersinia pestis EV76 strain with has no effect on cell viability is characterized by compliance effective maturation, manifested...
ABSTRACT Three plasmids expressing derivatives of the Yersinia pestis capsular F1 antigen were evaluated for their potential as DNA vaccines. These included full-length F1, devoid its putative signal peptide (deF1), and fused to signal-bearing E3 polypeptide Semliki Forest virus (E3/F1). Expression these in transfected HEK293 cells revealed that deF1 is expressed cytosol, E3/F1 targeted secretory cisternae, nonmodified rapidly eliminated from cell. Intramuscular vaccination mice with vector...
Prompt and effective elicitation of protective immunity is highly relevant for cases rapidly deteriorating fatal diseases, such as plague, which caused by
The global increase in multidrug-resistant (MDR) pathogenic bacteria has led to growing interest bacteriophage (“phage”) therapy. Therapeutic phages are usually selected based on their ability infect and lyse target bacteria, using vitro assays. In these assays, phage infection is determined grown standard commercial rich media, while evaluation of the actual therapeutic activity requires presence human blood. present work, we characterized two different Yersinia pestis lytic (ϕA1122 PST)...
Plague pandemics and outbreaks have killed millions of people during the history humankind. The disease, caused by bacteria Yersinia pestis, is currently treated effectively with antibiotics. However, in case multidrug-resistant (MDR) bacteria, alternative treatments are required. Bacteriophage (phage) therapy has shown efficient antibacterial activity various experimental animal models human patients infected different MDR pathogens. Here, we evaluated efficiency фA1122 PST phage therapy,...
The generation of adaptive immunity by vaccination is usually a prolonged process that requires multiple dosing over several months. Hence, vaccines are administered for disease prevention relatively long time prior to possible infection as opposed post-exposure prophylaxis, which typically rapid intervention such antibiotic therapy. emergence pathogens resistant common treatments has prompted the search alternative therapeutic strategies. We previously demonstrated mice with F1 capsular...
ABSTRACT Multiplexed detection technologies are becoming increasingly important given the possibility of bioterrorism attacks, for which range suspected pathogens can vary considerably. In this work, we describe use Luminex MagPlex magnetic microspheres construction two multiplexed diagnostic suspension arrays, enabling antibody-based bacterial and their related disease biomarkers directly from blood cultures. The first 4-plex array enabled both anthrax plague infections using soluble...
Markers of the early stages plague, a rapidly progressing deadly disease, are crucial for enabling onset an effective treatment. Here, we show that V-antigen protein (LcrV) is accumulated in serum Yersinia pestis-infected mice before bacterial colonization spleen and dissemination to blood, model bubonic plague. LcrV accumulation detected earlier than F1 capsular antigen, established marker disease. In mouse pneumonic can be determined bronchoalveolar lavage fluid somewhat later F1, but Y....
Bacterial infection of the lungs triggers a swift innate immune response that involves production cytokines and chemokines promote recruitment cells from bone marrow (BM) into infected tissue limit ability pathogen to replicate. Recent in vivo studies pneumonic plague animal models indicate pulmonary pro-inflammatory airway with Yersinia pestis is substantially delayed comparison other pathogens. Consequently, uncontrolled proliferation observed, followed by dissemination internal organs...
Great efforts are being made to develop new rapid antibiotic susceptibility tests meet the demand for clinical relevance versus disease progression. This is important especially in diseases caused by bacteria, such as Yersinia pestis, causative agent of plague, which grows rapidly vivo but relatively slow vitro. compromises ability use standard growth-based obtain and proper treatment guidance. Using our previously described platform quantifying antibiotic-specific transcriptional changes,...
Recent advances in the field of cell therapy have proposed new solutions for tissue repair and regeneration using various delivery approaches. Here we studied ex vivo a novel topical system encapsulated cells hybrid polyethylene glycol-fibrinogen (PEG-Fb) hydrogel microspheres to respiratory tract models. We investigated basic parameters encapsulation, release conditions inflamed damaged lungs bacterial-infected mice. The establishment each step study was essential proof concept....
Pneumonic plague is an infectious disease characterized by rapid and fulminant development of acute pneumonia septicemia that results in death within days exposure. The causative agent pneumonic plague, Yersinia pestis (Y. pestis), a Tier-1 bio-threat agent. Parenteral antibiotic treatment effective when given narrow therapeutic window after symptom onset. However, the non-specific "flu-like" symptoms often lead to delayed diagnosis therapy. In this study, we evaluated inhalational...