A5087993027- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Bone and Dental Protein Studies
- Bacillus and Francisella bacterial research
- Bacterial Genetics and Biotechnology
- Biosensors and Analytical Detection
- Yersinia bacterium, plague, ectoparasites research
- Animal Virus Infections Studies
- Cancer Cells and Metastasis
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Axon Guidance and Neuronal Signaling
- Digestive system and related health
- Wnt/β-catenin signaling in development and cancer
- Cancer Immunotherapy and Biomarkers
- COVID-19 and healthcare impacts
- RNA and protein synthesis mechanisms
- Neurofibromatosis and Schwannoma Cases
- Computational Drug Discovery Methods
- Viral Infections and Immunology Research
- Infection Control and Ventilation
- Insect and Pesticide Research
- Protein Structure and Dynamics
Israel Institute for Biological Research
2016-2024
Tel Aviv University
2015
Weizmann Institute of Science
2008-2015
Ben-Gurion University of the Negev
2009
Weatherford College
2008
Heidelberg University
1964
Kiel University
1964
Abstract Cancer-Associated Fibroblasts (CAFs) were shown to orchestrate tumour-promoting inflammation in multiple malignancies, including breast cancer. However, the molecular pathways that govern inflammatory role of CAFs are poorly characterised. In this study we found fibroblasts sense damage-associated patterns (DAMPs), and response activate NLRP3 inflammasome pathway, resulting instigation pro-inflammatory signalling secretion IL-1β. This upregulation was evident mouse human carcinomas....
Abstract The COVID-19 pandemic caused by SARS-CoV-2 imposes an urgent need for rapid development of efficient and cost-effective vaccine, suitable mass immunization. Here, we show the a replication competent recombinant VSV-∆G-spike in which glycoprotein VSV is replaced spike protein SARS-CoV-2. In-vitro characterization this vaccine indicates expression presentation on viral membrane with antigenic similarity to A golden Syrian hamster in-vivo model implemented. We that single-dose...
Kidney failure is frequently observed during and after COVID-19, but it remains elusive whether this a direct effect of the virus. Here, we report that SARS-CoV-2 directly infects kidney cells associated with increased tubule-interstitial fibrosis in patient autopsy samples. To study effects virus on independent systemic infected human-induced pluripotent stem-cell-derived organoids SARS-CoV-2. Single-cell RNA sequencing indicated injury dedifferentiation activation profibrotic signaling...
We report the results of COVID Moonshot, a fully open-science, crowdsourced, and structure-enabled drug discovery campaign targeting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) main protease. discovered noncovalent, nonpeptidic inhibitor scaffold with lead-like properties that is differentiated from current protease inhibitors. Our approach leveraged crowdsourcing, machine learning, exascale molecular simulations, high-throughput structural biology chemistry. generated...
Breast tumors are characterized by an extensive desmoplastic stroma, abundantly populated fibroblasts. Cancer-associated fibroblasts (CAF) support tumorigenesis stimulating angiogenesis, cancer cell proliferation, and invasion. CAF also orchestrate tumor-promoting inflammation in multiple tumor types, including breast cancer. However, the mechanisms through which normal tissue reprogrammed to CAFs mainly obscure. Here, we show that mammary can be educated cells become activated a...
Environmental surfaces have been suggested as likely contributors in the transmission of COVID-19. This study assessed infectivity severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contaminating and objects two hospital isolation units a quarantine hotel.
Melanoma is the leading cause of skin cancer mortality. The major melanoma mortality metastasis to distant organs, frequently brain. microenvironment plays a critical role in tumourigenesis and metastasis. In order treat or prevent metastasis, interactions disseminated tumour cells with at metastatic organ have be elucidated. However, brain stromal facilitating growth poorly understood. Astrocytes are glial that function repair scarring following injury, part via mediating neuroinflammation,...
Summary The ongoing SARS-CoV-2 pandemic has already caused devastating losses. Exponential spread can be slowed by social distancing and population-wide isolation measures, but those place a tremendous burden on society, and, once lifted, exponential re-emerge. Regular population-scale testing, combined with contact tracing case isolation, should help break the cycle of transmission, current detection strategies are not capable such large-scale processing. Here we present protocol for...
Frequent testing of large population groups combined with contact tracing and isolation measures will be crucial for containing Coronavirus Disease 2019 outbreaks. Here we present LAMP-Seq, a modified, highly scalable reverse transcription loop-mediated isothermal amplification (RT-LAMP) method. Unpurified biosamples are barcoded amplified in single heat step, pooled products analyzed en masse by sequencing. Using commercial reagents, LAMP-Seq has limit detection ~2.2 molecules per µl at 95%...
Hyperactivation of beta-catenin-T-cell-factor (TCF)-regulated gene transcription is a hallmark colorectal cancer (CRC). The cell-neural adhesion molecule L1CAM (hereafter referred to as L1) target beta-catenin-TCF, exclusively expressed at the CRC invasive front in humans. L1 overexpression cells increases cell growth and motility, promotes liver metastasis. Genes induced by are also human tissue but mechanisms which confers metastasis still unknown. We found that signaling nuclear factor...
Public health experts emphasize the need for quick, point-of-care SARS-CoV-2 detection as an effective strategy controlling virus spread. To this end, many "antigen" devices were developed and commercialized. These are mostly based on detecting SARS-CoV-2's nucleocapsid protein. Recently, alerts issued by both FDA CDC raised concerns regarding devices' tendency to exhibit false positive results. In work, we a novel alternative spike-based antigen assay, comprising four high-affinity,...
Translation of SARS-CoV-2-encoded mRNAs by the host ribosomes is essential for its propagation. Following infection, early expressed viral protein NSP1 binds ribosome, represses translation, and induces mRNA degradation, while elicits an anti-viral response. The mechanisms enabling to escape this multifaceted repression remain obscure. Here we show that expression leads destabilization multi-exon cellular mRNAs, intron-less transcripts, such as interferon genes, relatively stable. We...
The emergence of rapidly spreading variants Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) poses a major challenge to vaccines’ protective efficacy. Intramuscular (IM) vaccine administration induces short-lived immunity but does not prevent infection and transmission. New vaccination strategies are needed extend the longevity protection, induce mucosal systemic viral intranasal (IN) VSV–ΔG–spike candidate directly surfaces yielded superior at lower doses. Compared IM in...
A common challenge in drug design pertains to finding chemical modifications a ligand that increases its affinity the target protein. An underutilized advance is increase structural biology throughput, which has progressed from an artisanal endeavor monthly throughput of hundreds different ligands against protein modern synchrotrons. However, missing piece framework turns high-throughput crystallography data into predictive models for design. Here, we designed simple machine learning...
Abstract The transmembrane neural cell adhesion receptor L1 is a Wnt/β-catenin target gene expressed in many tumor types. In human colorectal cancer, localizes preferentially to the invasive front of tumors and when overexpressed cancer cells, it facilitates their metastasis liver. this study, we investigated genes that are regulated by L1-NF-κB pathway has been implicated liver metastasis. c-Kit was most highly suppressed both tissue pathway. suppression resulted from L1-mediated signaling...
Abstract The COVID-19 pandemic caused by SARS-CoV-2 that emerged in December 2019 China resulted over 7.8 million infections and 430,000 deaths worldwide, imposing an urgent need for rapid development of efficient cost-effective vaccine, suitable mass immunization. Here, we generated a replication competent recombinant VSV-ΔG-spike which the glycoprotein VSV was replaced spike protein SARS-CoV-2. In vitro characterization indicated expression presentation on viral membrane with antigenic...
SARS-CoV-2, the etiologic agent of COVID-19 pandemic, emerged as cause a global crisis. Rapid and reliable clinical diagnosis is essential for effectively controlling transmission. The gold standard assay SARS-CoV-2 identification highly sensitive real-time quantitative polymerase chain reaction (RT-qPCR); however, this depends on specialized reagents may suffer from false results. Thus, additional assays based different approaches could be beneficial. Here, we present novel method mass...
ABSTRACT Efficient treatment of anthrax-related meningitis in patients poses a significant therapeutic challenge. Previously, we demonstrated our anthrax rabbit model that ciprofloxacin is ineffective with most the treated animals succumbing to infection. Herein tested efficacy doxycycline and found it highly effective. Since all findings are based on model, test or specific central nervous system (CNS) developed non-human primates (NHPs). Similar rabbits, was ineffective, while protected...
SARS-CoV-2 Omicron strain emergence raised concerns that its enhanced infectivity is partly due to altered spread/contamination modalities. We therefore sampled high-contact surfaces and air in close proximity patients who were verified as infected with the strain, using identical protocols applied sample positive original or Alpha strains. Cumulatively, for all 3 strains, viral RNA was detected 90 of 168 6 49 samples (mean cycle threshold [Ct]=35.2±2.5). No infective virus identified....
Protective antigen (PA)-based vaccines are effective in preventing the development of fatal anthrax disease both humans and relevant animal models. The Bacillus anthracis toxins lethal toxin (lethal factor [LF] plus PA) edema (edema [EF] essential for establishment infection, as inactivation these results attenuation pathogen. Since reach high toxemia levels at bacteremic stages disease, CDC's recommendations include combining antibiotic treatment with antitoxin (anti-PA) immunotherapy. We...