Jitske Jansen
A5038195774- Renal and related cancers
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Renal Diseases and Glomerulopathies
- Tissue Engineering and Regenerative Medicine
- Renal cell carcinoma treatment
- Chronic Kidney Disease and Diabetes
- Genetic and Kidney Cyst Diseases
- COVID-19 and healthcare impacts
- Drug Transport and Resistance Mechanisms
- Dialysis and Renal Disease Management
- Biomedical Research and Pathophysiology
- Single-cell and spatial transcriptomics
- Cancer Cells and Metastasis
- Organ Donation and Transplantation
- Electrospun Nanofibers in Biomedical Applications
- Computational Drug Discovery Methods
- Hemoglobinopathies and Related Disorders
- Systemic Sclerosis and Related Diseases
- 3D Shape Modeling and Analysis
- Ion Transport and Channel Regulation
- Additive Manufacturing and 3D Printing Technologies
- Diet and metabolism studies
- Complement system in diseases
- Autophagy in Disease and Therapy
RWTH Aachen University
1998-2025
Universitätsklinikum Aachen
2023-2024
Erasmus MC
2023-2024
Radboud University Medical Center
2013-2023
Radboud Institute for Molecular Life Sciences
2014-2023
Radboud University Nijmegen
2013-2023
University Medical Center
2022-2023
Erasmus University Rotterdam
2023
Roche (Switzerland)
2023
Utrecht University
2017-2022
Kidney failure is frequently observed during and after COVID-19, but it remains elusive whether this a direct effect of the virus. Here, we report that SARS-CoV-2 directly infects kidney cells associated with increased tubule-interstitial fibrosis in patient autopsy samples. To study effects virus on independent systemic infected human-induced pluripotent stem-cell-derived organoids SARS-CoV-2. Single-cell RNA sequencing indicated injury dedifferentiation activation profibrotic signaling...
We report the results of COVID Moonshot, a fully open-science, crowdsourced, and structure-enabled drug discovery campaign targeting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) main protease. discovered noncovalent, nonpeptidic inhibitor scaffold with lead-like properties that is differentiated from current protease inhibitors. Our approach leveraged crowdsourcing, machine learning, exascale molecular simulations, high-throughput structural biology chemistry. generated...
Abstract The development of a biotechnological platform for the removal waste products (e.g. uremic toxins), often bound to proteins in plasma, is prerequisite improve current treatment modalities patients suffering from end stage renal disease (ESRD). Here, we present newly designed bioengineered tubule capable active toxin secretion through concerted action essential transporters, viz. organic anion transporter-1 (OAT1), breast cancer resistance protein (BCRP) and multidrug protein-4...
Membrane transporters and receptors are responsible for balancing nutrient metabolite levels to aid body homeostasis. Here, we report that proximal tubule cells in kidneys sense elevated endogenous, gut microbiome-derived, through EGF downstream signaling induce their secretion by up-regulating the organic anion transporter-1 (OAT1). Remote sensing was observed from healthy volunteers rats vivo, leading induced OAT1 expression increased removal of indoxyl sulfate, a prototypical...
Kidney organoids generated from induced pluripotent stem cells (iPSC) have proven valuable for studies of kidney development, disease, and therapeutic screening. However, specific applications been hampered by limited expansion capacity, immaturity, off-target cells, inability to access the apical side. Here, we apply recently developed tubuloid protocols purify propagate epithelium d7+18 (post nephrogenesis) iPSC-derived organoids. The resulting ‘iPSC organoid-derived (iPSCod)’ tubuloids...
Although there has been increasing interest in the use of high protein diets, little is known about dietary related changes mammalian metabolome. We investigated influence intake on selected tryptophan and phenolic compounds, derived from both endogenous colonic microbial metabolism. Furthermore, potential inter-species metabolic differences were studied. For this purpose, 29 healthy subjects allocated to a (n = 14) or low diet 15) for 2 weeks. In addition, 20 wild-type FVB mice randomized...
Introduction: To date, tubular tissue engineering relies on large, non-porous scaffolds (Ø > 2 mm) for mechanical self-support, or smaller 150–500 μm) tubes within bulk hydrogels studying renal transport phenomena. advance the of kidney tubules future implantation, constructs should be both self-supportive and yet small-sized highly porous. Here, we hypothesize that fabrication porous with a organized fibrous microstructure by means melt-electrowriting (MEW) allows development...
Progressive respiratory failure is the primary cause of death in coronavirus disease 2019 (COVID-19) pandemic. It final outcome acute distress syndrome (ARDS), characterized by an initial exacerbated inflammatory response, metabolic derangement and ultimate tissue scarring. A positive balance cellular energy may result crucial for recovery clinical COVID-19. Hence, we asked if two key pathways involved generation, AMP-activated protein kinase (AMPK)/acetyl-CoA carboxylase (ACC) signaling...
Background. Atypical HUS (aHUS) is thought to be caused by predisposing mutations in genes encoding complement (regulating) proteins, such as Factor H (CFH), I (IF), membrane co-factor protein (MCP) and B (FB), or auto-antibodies against CFH (αFH) combination with a homozygous polymorphic deletion of the Complement H-related 1 3 (ΔCFHR1/3). The clinical impact this knowledge high, it might prognostic factor for outcome renal transplantations kidney donations. Methods. Mutational screening,...
Nephrotic syndrome (NS) is characterized by severe proteinuria as a consequence of kidney glomerular injury due to podocyte damage. In vitro models mimicking in vivo characteristics are prerequisite resolve NS pathogenesis. The detailed characterization organoid podocytes resulting from hybrid culture protocol showed population that resembles adult and was superior compared with 2D counterparts, based on single-cell RNA sequencing, super-resolution imaging electron microscopy. this study,...
Abstract Recreating human tissues and organs in the petri dish to establish models as tools biomedical sciences has gained momentum. These can provide insight into mechanisms of physiology, disease onset, progression, improve drug target validation, well development new medical therapeutics. Transformative materials play an important role this evolution, they be programmed direct cell behavior fate by controlling activity bioactive molecules material properties. Using nature inspiration,...
Abstract Despite the increasing incidence of kidney-related diseases, we are still far from understanding underlying mechanisms these diseases and their progression. This lack is partly because a poor replication in vitro, limited to planar culture. Advancing towards three-dimensional models, hereby propose coaxial printing obtain microfibers containing helical hollow microchannel. These recapitulate architecture proximal tubule (PT), an important nephron segment often affected kidney...
A common challenge in drug design pertains to finding chemical modifications a ligand that increases its affinity the target protein. An underutilized advance is increase structural biology throughput, which has progressed from an artisanal endeavor monthly throughput of hundreds different ligands against protein modern synchrotrons. However, missing piece framework turns high-throughput crystallography data into predictive models for design. Here, we designed simple machine learning...