A5005055310- RNA modifications and cancer
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Cancer-related gene regulation
- Viral Infections and Immunology Research
- RNA regulation and disease
- SARS-CoV-2 and COVID-19 Research
- Genomics and Chromatin Dynamics
- Virus-based gene therapy research
- Endoplasmic Reticulum Stress and Disease
- interferon and immune responses
- Mitochondrial Function and Pathology
- Cancer-related molecular mechanisms research
- Bone Metabolism and Diseases
- RNA Interference and Gene Delivery
- Spondyloarthritis Studies and Treatments
- Cancer-related Molecular Pathways
- Genetics and Neurodevelopmental Disorders
- Fungal and yeast genetics research
- Bone and Joint Diseases
- Epigenetics and DNA Methylation
Technion – Israel Institute of Technology
2023-2024
Weizmann Institute of Science
2010-2023
The Netherlands Cancer Institute
2015-2017
Tel Aviv University
2007
Transcription and translation are two main pillars of gene expression. Due to the different timings, spots action, mechanisms regulation, these processes mainly regarded as distinct generally uncoupled, despite serving a common purpose. Here, we sought for possible connection between transcription translation. Employing an unbiased screen multiple human promoters, identified positive effect TATA box on general coupling mRNA expression translational efficiency. Using CRISPR-Cas9-mediated...
Intracellular mRNA targeting and localized translation are potential determinants for protein localization. To facilitate targeting, mRNAs possess specific cis -acting sequence motifs that recognized by trans RNA-binding proteins (RBPs). While many trafficked, our knowledge of the RBPs involved presence additional transcripts within these ribonucleoprotein (RNP) complexes is limited. identification bind to mRNAs, we developed R N A -binding p rotein purification id entification (RaPID), a...
mRNAs encoding secreted/membrane proteins (mSMPs) are believed to reach the endoplasmic reticulum (ER) in a translation-dependent manner confer protein translocation. Evidence exists, however, for translation- and signal recognition particle (SRP)-independent mRNA localization ER, suggesting that there alternate paths RNA delivery. We localized endogenously expressed mSMPs yeast using an aptamer-based RNA-tagging procedure fluorescence microscopy. Unlike polarity secretion factors colocalize...
Translation of SARS-CoV-2-encoded mRNAs by the host ribosomes is essential for its propagation. Following infection, early expressed viral protein NSP1 binds ribosome, represses translation, and induces mRNA degradation, while elicits an anti-viral response. The mechanisms enabling to escape this multifaceted repression remain obscure. Here we show that expression leads destabilization multi-exon cellular mRNAs, intron-less transcripts, such as interferon genes, relatively stable. We...
m6A is the most widespread mRNA modification and primarily implicated in controlling stability. Fundamental questions pertaining to are extent which it dynamically modulated within cells across stimuli, forces underlying such modulation. Prior work has focused on investigating active mechanisms governing levels, as recruitment of writers or erasers leading either ‘global’ ‘site-specific’ Here, we propose that changes levels subcellular compartments biological trajectories may result from...
Nuclear-encoded mRNAs encoding mitochondrial proteins (mMPs) can localize directly to the surface, yet how mMPs target mitochondria and whether RNA targeting contributes protein import into cellular metabolism are unknown. Here, we show that COPI vesicle coat complex is necessary for mMP localization function. inactivation leads reduced binding itself, resulting in dissociation of from mitochondria, a reduction membrane potential, decrease vivo vitro, severe deficiencies respiration. Using...
PP2Cα is the representative member of type 2C family protein phosphatases, and it has recently been implicated in regulation p53-, TGFβ-, cyclin-dependent kinase- apoptosis-signaling. To investigate role cell growth radio- chemosensitivity, wild siRNA-expressing MCF7 cells were subjected to several different viability cycle analyses, both under basal conditions upon treatment with chemotherapy. By comparing tumors established from types cells, we also evaluated involvement tumorigenesis. It...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of ongoing disease 19 (COVID-19) pandemic. Despite urgent need, we still do not fully understand molecular basis SARS-CoV-2 pathogenesis and its ability to antagonize innate immune responses. Here, use RNA-sequencing ribosome profiling along infection comprehensively define mechanisms that are utilized by shutoff cellular protein synthesis. We show leads a global reduction in translation but viral transcripts...
Abstract Translation of SARS-CoV-2-encoded mRNAs by the host ribosomes is essential for its propagation. Following infection, early expressed viral protein NSP1 binds ribosome, represseses translation and induces mRNA degradation, while elicits anti-viral response. The mechanisms enabling to escape this multifaceted repression remain obscure. Here we show that expression leads destabilization multi-exon cellular mRNAs, intron-less transcripts, such as interferon genes, relatively stable. We...
Abstract m6A is the most widespread mRNA modification and primarily implicated in controlling stability. Fundamental questions pertaining to are extent which it dynamically modulated within cells across stimuli, forces underlying such modulation. Prior work has focused on investigating active mechanisms governing levels, as recruitment of writers or erasers leading either ‘global’ ‘site-specific’ Here, we propose that changes levels subcellular compartments biological trajectories may result...
m6A is the most widespread mRNA modification and primarily implicated in controlling stability. Fundamental questions pertaining to are extent which it dynamically modulated within cells across stimuli, forces underlying such modulation. Prior work has focused on investigating active mechanisms governing levels, as recruitment of writers or erasers leading either ‘global’ ‘site-specific’ Here, we propose that changes levels subcellular compartments biological trajectories may result from...
m6A is the most widespread mRNA modification and primarily implicated in controlling stability. Fundamental questions pertaining to are extent which it dynamically modulated within cells across stimuli, forces underlying such modulation. Prior work has focused on investigating active mechanisms governing levels, as recruitment of writers or erasers leading either ‘global’ ‘site-specific’ Here, we propose that changes levels subcellular compartments biological trajectories may result from...
Abstract N6-methyladenosine (m6A), a widespread destabilizing mark on mRNA, is non-uniformly distributed across the transcriptome, yet basis for its selective deposition unknown. Here, we uncover that m6A not selective. Instead, distribution exclusion-based: m6A-consensus harboring sites are methylated by default, unless they within window of up to ∼200 nt from an exon-intron junction. A simple model, relying exclusively presence motifs and architecture allows high accuracy recapitulation...