A5021643357- Cytomegalovirus and herpesvirus research
- Genomics and Chromatin Dynamics
- Retinal Development and Disorders
- Nerve injury and regeneration
- Monoclonal and Polyclonal Antibodies Research
- Herpesvirus Infections and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA and protein synthesis mechanisms
- Neurogenesis and neuroplasticity mechanisms
- SARS-CoV-2 and COVID-19 Research
- RNA Research and Splicing
- HIV Research and Treatment
- Viral-associated cancers and disorders
- interferon and immune responses
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Calpain Protease Function and Regulation
- Laser Applications in Dentistry and Medicine
- Mycobacterium research and diagnosis
- Ocular and Laser Science Research
- Genetics and Neurodevelopmental Disorders
- DNA Repair Mechanisms
- Glaucoma and retinal disorders
- Glycosylation and Glycoproteins Research
- Viral gastroenteritis research and epidemiology
Weizmann Institute of Science
2010-2024
Forest Institute
2024
New York Academy of Sciences
2023
John Wiley & Sons (Germany)
2023
Moss Landing Marine Laboratories
2023
Hudson Institute
2023
Bar-Ilan University
2014-2022
Goodman (Japan)
2017
University of California, San Diego
2014-2015
La Jolla Alcohol Research
2015
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of ongoing disease 2019 (COVID-19) pandemic1. To understand pathogenicity and antigenic potential SARS-CoV-2 to develop therapeutic tools, it essential profile full repertoire its expressed proteins. The current map coding capacity based on computational predictions relies homology with other coronaviruses. As protein complement varies among coronaviruses, especially in regard variety accessory proteins, crucial...
Primary infection with human cytomegalovirus (HCMV) results in a lifelong due to its ability establish latent infection, one characterized viral reservoir being hematopoietic cells. Although reactivation from latency causes serious disease immunocompromised individuals, our molecular understanding of is limited. Here, we delineate gene expression during natural HCMV persistent by analyzing the massive transcriptome RNA sequencing (RNA-seq) atlas generated Genotype-Tissue Expression (GTEx)...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) leads to shutoff of protein synthesis, and nsp1, a central factor in coronaviruses, inhibits cellular mRNA translation. However, the diverse molecular mechanisms employed by nsp1 as well its functional importance are unresolved. By overexpressing various mutants generating SARS-CoV-2 mutant, we show that through inhibition translation induction degradation, targets translated is main driver host during infection. The propagation...
Common fragile sites are specific loci that form gaps and constrictions on metaphase chromosomes exposed to replication stress, which slows DNA replication. These have a role in chromosomal rearrangements tumors; however, the molecular mechanism of their expression is unclear. Here we show stress leads focus formation Rad51 phosphorylated DNA-PKcs, key components homologous recombination (HR) nonhomologous end-joining (NHEJ), double-strand break (DSB) repair pathways, respectively....
The involvement of gangliosides in processes related to nerve regeneration and sprouting has been demonstrated recently. type interaction by which may influence neuronal was investigated the present work. Affinity-purified rabbit anti-GM1 antibodies were found block from dorsal root ganglia (DRG) chicken embryo induced growth factor (NGF). Only a moderately inhibitory effect produced directed GM2, suggesting specific between NGF GM1. In spite low anti-GM2 on sprouting, their binding...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of ongoing Coronavirus disease 19 (COVID-19) pandemic 1,2 . In order to understand SARS-CoV-2 pathogenicity and antigenic potential, develop diagnostic therapeutic tools, it essential portray full repertoire its expressed proteins. The coding capacity map currently based on computational predictions relies homology other coronaviruses. Since coronaviruses differ in their protein array, especially variety...
Human cytomegalovirus (HCMV) causes a lifelong infection through establishment of latency. Although reactivation from latency can cause life-threatening disease, our molecular understanding HCMV is incomplete. Here we use single cell RNA-seq analysis to characterize in monocytes and hematopoietic stem progenitor cells (HSPCs). In monocytes, identify host surface markers that enable enrichment latent harboring higher viral transcript levels, which reactivate more efficiently, are...
During productive human cytomegalovirus (HCMV) infection, viral genes are expressed in a coordinated cascade that conventionally relies on the dependencies of protein synthesis and DNA replication. By contrast, transcriptional landscape HCMV latency is poorly understood. Here, we examine gene expression dynamics during establishment both latent infections. We redefine kinetics infection reveal regulation does not represent simple sequential cascade; many regulated by multiple independent...
Abstract Low‐energy He‐Ne laser irradiation (LELI) was found to affect the electric activity and morphology in both intact severely injured peripheral nerves rats. Action potential (AP) healthy nerve increased by 33% following a single transcutaneous irradiation. Similar crushed caused AP increase significantly over of nonirradiated nerve. Morphological observations revealed that laser‐irradiated had diminished scar tissue as compared an but not irradiated
Retinal neurogenesis ceases by the early postnatal period, although retinal progenitor cells (RPCs) persist throughout life. In this study, we show that in mammalian eye, function of Toll-like receptor 4 (TLR4) extends beyond regulation innate immune response; it restricts RPC proliferation. TLR4-deficient mice, enhanced proliferation reminiscent RPCs is evident during period. vitro experiments demonstrate TLR4 acts as an intrinsic regulator fate decision. Increased expression eye correlates...
Viruses are known for their extremely compact genomes composed almost entirely of protein-coding genes. Nonetheless, four long noncoding RNAs (lncRNAs) encoded by human cytomegalovirus (HCMV). Although these accumulate to high levels during lytic infection, functions remain largely unknown. Here, we show that HCMV-encoded lncRNA4.9 localizes the viral nuclear replication compartment, and its depletion restricts DNA growth. RNA4.9 is transcribed from HCMV origin (oriLyt) forms an RNA-DNA...
Significance Human cytomegalovirus (HCMV) reactivation is a major cause of posttransplant morbidity/mortality. One approach toward reducing this purging the transplant donor and/or recipient latently infected cells prior to stem-cell or organ harvest/engraftment. Our findings show involvement host bromodomain (BRD) proteins in regulation HCMV latency and reactivation. Bromodomain extra-terminal inhibitor (I-BET) treatment causes lytic gene expression by release transcription activator P-TEFb...
HU-211 is a novel synthetic analog of tetrahydrocannabinol that was recently shown in animal models to be nonpsychotropic. In this study we show can potentially used as neuroprotective compound the CNS. Using calibrated crush injury adult rat optic nerve, reduce injury-induced metabolic and electrophysiological deficits. Energy metabolism monitored by measuring intramitochondrial nicotine-amine adenine dinucleotide redox state hourly for 6 h after treatment. Electrophysiological activity...
There is a growing interest in the role of chromatin acquiring and maintaining cell identity. Despite ever-growing availability genome-wide gene expression data, understanding how transcription programs are established regulated to define identity remains puzzle. An important mechanism regulation binding factors (TFs) specific DNA sequence motifs across genome. However, these sequences hindered by packaging chromatin. Thus, accessibility loci for TF highly determines where when TFs bind. We...