A5039508751- Cytomegalovirus and herpesvirus research
- HIV Research and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Single-cell and spatial transcriptomics
- Lysosomal Storage Disorders Research
- Mycobacterium research and diagnosis
- Advanced biosensing and bioanalysis techniques
- Hepatitis C virus research
- Lipid Membrane Structure and Behavior
- Sphingolipid Metabolism and Signaling
- CRISPR and Genetic Engineering
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Herpesvirus Infections and Treatments
- SARS-CoV-2 and COVID-19 Research
Weizmann Institute of Science
2020-2024
The backbone of all sphingolipids (SLs) is a sphingoid long-chain base (LCB) to which fatty acid N-acylated. Considerable variability exists in the chain length and degree saturation both these hydrophobic chains, recent work has implicated ceramides with different LCBs N-acyl chains distinct biological processes; moreover, they may play roles disease states possibly even act as prognostic markers. We now demonstrate that half-life, or turnover rate, containing diverse different. By means...
Abstract Human Cytomegalovirus (HCMV) infection can result in either productive or latent infection, the latter being basis for virus life-long persistence. Intriguingly, monocytes, which support become permissive to upon differentiation macrophages. However, molecular factors explaining these differentiation-driven differences are not fully understood and have been so far attributed chromatin-mediated repression of viral genome. Here, by using metabolic labeling newly synthesized RNA early...
Abstract Genetic screens have transformed our ability to interrogate cellular factor requirements in infection, yet current approaches are limited their sensitivity, biased towards early stages of infection and provide only simplistic phenotypic information which is often based on infected cell survival. Here, by engineering human cytomegalovirus express sgRNA libraries directly from the viral genome, we developed a sensitive, versatile, centric approach that allows profiling different along...
Abstract Infection with Human cytomegalovirus (HCMV) can result in either productive or non-productive infection, the latter potentially leading to establishment of latency, but molecular factors that dictate these different infection outcomes are elusive. Macrophages known targets HCMV and considered be permissive for while monocytes, their precursors, latently infected. Here we reveal macrophages is more complex than previously appreciated infection. By analyzing progression monocytes...