This article describes a simple and selective procedure used for direct measurement of sulfated glycosaminoglycans (GAGs) in biological samples its application to the determination GAGs during tissue regeneration myogenic differentiation. We describe modified previous GAG assays that has improved specificity, reproducibility, sensitivity. The assay is based on ability bind cationic dye 1,9-dimethylmethylene blue. conditions allow isolation GAG-dye complex. complex was dissociated; optical...

Abstract Exon junction complexes (EJCs) mark untranslated spliced mRNAs and are crucial for the mRNA lifecycle. An imbalance in EJC dosage alters mouse neural stem cell (mNSC) division is linked to human neurodevelopmental disorders. In quiescent mNSC immortalized retinal pigment epithelial (RPE1) cells, centrioles form a basal body ciliogenesis. Here, we report that EJCs accumulate at bodies of or RPE1 cells decline when these differentiate resume growth. A high-throughput smFISH screen...

Abstract The nuclear Cap-Binding Complex (CBC), consisting of Nuclear Protein 1 (NCBP1) and 2 (NCBP2), associates with the nascent 5′cap RNA polymerase II transcripts impacts fate decisions. Recently, C17orf85 protein, also called NCBP3, was suggested to form an alternative CBC by replacing NCBP2. However, applying protein–protein interaction screening NCBP1, 3, we find that profile NCBP3 is distinct. Whereas NCBP1 identify known interactors, primarily interacts components Exon Junction...

The exon junction complex (EJC) deposited on spliced mRNAs, plays a central role in the post-transcriptional gene regulation and specific expression. EJC core is associated with multiple peripheral factors involved various post-splicing events. Here, using recombinant reconstitution transcriptome-wide analysis, we showed that protein complexes ASAP PSAP form distinct can confer to EJCs alternative splicing regulatory activities. This study provides first evidence different have functions,...

Abstract Human CWC27 is an uncharacterized splicing factor and mutations in its gene are linked to retinal degeneration other developmental defects. We identify the CWC22 as major partner. Both present published Bact spliceosome structures, but no interacting domains visible. Here, structure of a CWC27/CWC22 heterodimer bound exon junction complex (EJC) core component eIF4A3 solved at 3Å-resolution. According spliceosomal EJC recruited C complex, once has left. Our 3D eIF4A3/CWC22/CWC27...

The Bacillus cereus sensu lato complex has recently been divided into several phylogenetic groups with clear differences in growth temperature range. However, only a few studies have investigated the actual pathogenic potential of psychrotolerant strains B. group at low temperature, and little information is available concerning gene expression temperature. We found that vegetative cells weihenstephanensis strain KBAB4 were against model insect Galleria mellonella 15°C but not 30°C. A...

ABSTRACT The mechanisms involved in the ability of Bacillus cereus to multiply at low temperatures were investigated. It was assumed that many genes cold acclimation would be upregulated temperatures. Recombinase-based vivo expression technology (IVET) adapted detection transient activation B. promoters during growth 10°C. Four independent screenings a promoter library from type strain ATCC 14579 performed, and 17 clones isolated. They corresponded regions displayed reproducibly elevated...

Production of reactive nitrogen species (NO) is a key step in the immune response following infections. NO induces lesions to bacterial DNA, thus limiting growth within hosts. Using two pathogenic bacteria, Bacillus cereus and Shigella flexneri, we show that DNA-repair protein Mfd (Mutation-Frequency-Decline) required for resistance host-NO-response. In both species, mutant deficient mfd does not survive NO, produced vitro or by phagocytic cells. vivo, ∆mfd avirulent unable NO-stress....

Terminal deoxynucleotidyltransferase (Tdt) and DNA polymerase μ (pol μ) are two eukaryotic highly similar proteins involved in processing repair. Despite their high sequence identity, they differ widely activity: pol has a templated activity, whereas Tdt non-templated one. Loop1, first described when the structure was solved, been invoked as major structural determinant of this difference. Here we describe attempts to transform into with minimal number mutations around Loop1. First effect on...

Abstract Exon junction complexes are deposited at exon-exon junctions during splicing. They primarily known to activate non-sense mediated degradation of transcripts harbouring premature stop codons before the last intron. According a popular model, exon-junction accompany mRNAs cytoplasm where first translating ribosome pushes them out. However, they also removed by uncharacterized, translation-independent mechanisms. Little is about kinetic and transcript specificity these processes. Here...

Crucial events in myogenesis rely on the highly regulated spatiotemporal distribution of cell surface heparan sulfate proteoglycans to which are associated growth factors, thus creating a specific microenvironment around muscle cells. Most factors involved control myoblast and differentiation stored extracellular matrix through interaction with sequences glycosaminoglycan oligosaccharides, mainly (HS). Different HS subspecies revealed by antibodies, have been shown provide regulation during...

Abstract Muscle regeneration occurs through the activation of satellite cells, which are stimulated to proliferate and fuse into myofibers that will reconstitute damaged muscle. We have previously reported a family new compounds called “regenerating agents” (RGTAs), polymers engineered mimic heparan sulfates, stimulate in vivo tissue repair. One these agents, RG1192, dextran derivative substituted by CarboxyMethyl, Benzylamide, Sulfate (noted CMBS, RGTA type), was shown improve greatly rat...

Estudo epidemiológico, transversal, quantitativo e descritivo com o objetivo de conhecer características sociodemográficas clínicas das mulheres que realizaram exame Papanicolaou coletado por enfermeiros da Estratégia Saúde Família no Município Novo Cruzeiro, Minas Gerais. Os dados secundários do Sistema Informação sobre Câncer Colo Útero Departamento Informática Ministério Saúde, período 2006 a 2009, foram analisados, totalizando 8.281 exames, na proporção 0,38 exames em 25 59 anos. A...

Resumo Objetivo Analisar a prevalência de excesso peso corporal em adolescentes e fatores associados. Métodos Estudo transversal conduzido com 635 ambos os sexos, idade entre 10 16 anos, escolas públicas estaduais. A coleta dados foi realizada no segundo semestre 2016 por uma equipe multidisciplinar. Coletou-se demográficos, da história familiar, desenvolvimento púbere hábitos comportamentais. Foi feita classificação dos adolescentes, conforme o estado nutricional. Utilizou-se análise...

Objetivou-se identificar os motivos de reprovações materiais médico-hospitalares (MMH) no edital licitação 001 e 029 /2011, na modalidade pregão eletrônico as categoriais profissionais envolvidas avaliação da qualidade dos médico-hospitalares. Trata-se um estudo quantitativo caráter descritivo, transversal documental. Os dados foram obtidos a partir formulários utilizados pelos com intuito averiguar tais presentes arquivo Comissão Padronização Materiais Produtos (COPAMP) hospital...

Abstract Exon junction complexes (EJC) mark untranslated spliced mRNAs and are crucial for the mRNA lifecycle. An imbalance in EJC dosage alters mouse neural stem cell (mNSC) division is linked to human neurodevelopmental disorders. In quiescent mNSC immortalized retinal pigment epithelial (RPE1) cells, centrioles form a basal body ciliogenesis. Here, we report that EJCs accumulate at bodies of or RPE1 cells decline when these differentiate resume growth. A high-throughput smFISH screen...

This paper aimed to analyze the direct and indirect effects of adiposity factors related adolescents' quality life. is a cross-sectional study with 635 adolescents (10-16 years) from public schools in Montes Claros-MG, Brazil. Anthropometric data, physical activity (PA), inadequate dietary habits, body image, life (QoL) were collected. A structural equation modeling was performed. The variables addressed as constructs exploratory PA outcome variable QoL. total effect PA-mediated on QoL...