A5047985373- Cardiac Fibrosis and Remodeling
- Neuropeptides and Animal Physiology
- Mitochondrial Function and Pathology
- DNA Repair Mechanisms
- Cardiac electrophysiology and arrhythmias
- Toxoplasma gondii Research Studies
- Glioma Diagnosis and Treatment
- Carcinogens and Genotoxicity Assessment
- Ion channel regulation and function
- Renin-Angiotensin System Studies
- Cell Adhesion Molecules Research
- Alzheimer's disease research and treatments
- Retinoids in leukemia and cellular processes
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer, Hypoxia, and Metabolism
- Signaling Pathways in Disease
- DNA and Nucleic Acid Chemistry
- Immune Cell Function and Interaction
- Cytomegalovirus and herpesvirus research
- Systemic Lupus Erythematosus Research
- Adipose Tissue and Metabolism
- Cardiac Ischemia and Reperfusion
- Receptor Mechanisms and Signaling
- Protein Tyrosine Phosphatases
- Herpesvirus Infections and Treatments
Oregon Health & Science University
2006-2020
Czech Academy of Sciences, Institute of Physiology
2016
Sympathetic and parasympathetic control of the heart is a classic example norepinephrine (NE) acetylcholine (ACh) triggering opposing actions. NE increases rate contractility through activation β receptors, whereas ACh slows muscarinic receptors. neurons can undergo developmental transition from production to we provide evidence that mouse cardiac sympathetic nerves transiently produce after myocardial infarction (MI). levels increased in viable tissue 10–14 d MI, returning at 21 d, were...
Toxoplasma gondii, the parasite responsible for ocular toxoplasmosis, accesses retina from bloodstream. We investigated dendritic cell as a potential taxi T. gondii tachyzoites moving across human retinal endothelium, and examined participation of adhesion molecules chemokines in this process.CD14-positive monocytes were isolated peripheral blood by antibody-mediated enrichment, cultured granulocyte-macrophage colony-stimulating factor interleukin-4 to generate cells. Transmigration assays...
Cardiac sympathetic nerves undergo cholinergic transdifferentiation following reperfused myocardial infarction (MI), whereby the release both norepinephrine (NE) and acetylcholine (ACh). The functional electrophysiological consequences of post-MI have never been explored. We performed MI or sham surgery in wild-type (WT) mice which choline acetyltransferase was deleted from adult noradrenergic neurons [knockout (KO)]. Electrophysiological activity assessed with optical mapping action...
The stellate ganglia are the predominant source of sympathetic innervation to heart. Remodeling nerves projecting heart has been observed in several cardiovascular diseases, however studies adult limited. A profile baseline transcriptomic and neurochemical characteristics C57Bl6j mice, a common model for study may aid future investigations. We have generated dataset measurements mouse using RNAseq, HPLC mass spectrometry. Expression differences between male female mice were identified. These...
Purpose: B cells participate in diverse retinal immunopathologies. Endothelial adhesion molecules and chemokines direct leukocyte trafficking. We examined the involvement of three molecular signals transendothelial migration human cells: ICAM-1, VCAM-1, CXCL13.Methods: Peripheral blood were isolated by negative selection. Migration was studied transwells populated with endothelial monolayers, using antibody to block ICAM-1 or VCAM-1. Retinal expression CXCL13 investigated.Results: crossed...
The genotoxicant methylazoxymethanol (MAM) is a widely used developmental neurotoxin, and its glucoside an etiological factor for western Pacific amyotrophic lateral sclerosis-parkinsonism-dementia complex (ALS/PDC). Identification of global protein expression changes that occur in response to MAM the developing cerebellum could provide valuable insight into potential mechanisms involved neurodegeneration process. We have utilized fluorescence 2-dimensional differential gel electrophoresis...
Cardiac sympathetic nerves stimulate heart rate and force of contraction. Myocardial infarction (MI) leads to the loss within heart, clinical studies have indicated that denervation is a risk factor for arrhythmias cardiac arrest. Two distinct types been identified in mouse after MI caused by ischemia-reperfusion: transient peri-infarct myocardium sustained infarct. Sustained linked increased arrhythmia risk, but it not known whether acute nerve also contributes risk. Peri-infarct requires...
Exposure of the brain to environmental agents during critical periods neuronal development is considered a key factor underlying many neurologic disorders.In this study we examined influence genotoxicants on cerebellar function early by measuring global gene expression changes.We measured in immature neurons (i.e., granule cells) after treatment with two distinct alkylating agents, methylazoxymethanol (MAM) and nitrogen mustard (HN2). Granule cell cultures were treated for 24 hr MAM...
The stellate ganglia are the predominant source of sympathetic innervation to heart. Remodeling nerves projecting heart has been observed in several cardiovascular diseases, and dysfunction contributes cardiac pathology. Wistar Kyoto rats a common model for study but we lack profile baseline transcriptomic neurochemical characteristics their neurons. Most studies disease have used male animals only, future both female will be these types studies; therefore, sought characterize transcriptome...
Survivors of a myocardial infarction (MI) remain at serious risk sudden cardiac death due to lethal arrhythmias. It is known from studies in humans and dogs that electrical structural remodeling the stellate sympathetic neurons occurs following MI, including an increase size excitability. Increased excitability may be variety sources increased dendritic field size, more synaptic inputs or altered ion channel expression name few. In effort identify neuronal genes regulated by we have...