A5049396071- Pharmacogenetics and Drug Metabolism
- Metabolism and Genetic Disorders
- Peroxisome Proliferator-Activated Receptors
- Analytical Chemistry and Chromatography
- Pharmacological Effects of Natural Compounds
- Cancer therapeutics and mechanisms
- Ovarian cancer diagnosis and treatment
- Nitric Oxide and Endothelin Effects
- Drug Transport and Resistance Mechanisms
- Forensic and Genetic Research
- Spine and Intervertebral Disc Pathology
- Hemoglobin structure and function
- Advanced Nanomaterials in Catalysis
- Advancements in Solid Oxide Fuel Cells
- Infant Nutrition and Health
- Medical and Biological Ozone Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Liver Disease Diagnosis and Treatment
- Financial Markets and Investment Strategies
- RNA Research and Splicing
- Musculoskeletal pain and rehabilitation
- Cancer-related molecular mechanisms research
- Electronic and Structural Properties of Oxides
- Eicosanoids and Hypertension Pharmacology
- Endometrial and Cervical Cancer Treatments
University of Pittsburgh
2008-2024
Wuhan University
2020-2024
Institute of Economics
2012-2023
Children's Hospital of Pittsburgh
2004-2023
Chinese Academy of Social Sciences
2012-2023
Huazhong University of Science and Technology
2022
Energy Foundation
2022
Luye Pharma (China)
2021
Nanjing Agricultural University
2021
Shanghai Jiao Tong University
2020
Background— Intravascular red cell hemolysis impairs nitric oxide (NO)–redox homeostasis, producing endothelial dysfunction, platelet activation, and vasculopathy. Red blood storage under standard conditions results in reduced integrity of the erythrocyte membrane, with formation exocytic microvesicles or microparticles hemolysis, which we hypothesized could impair vascular function contribute to putative lesion banked blood. Methods Results— We now find that human cells banking accumulation...
Abstract Chronic ethanol (EtOH) abuse results in the development of steatosis, alcoholic hepatitis, and cirrhosis. Augmented TNF-α production by macrophages Kupffer cells signaling via p55 TNF receptor have been shown to be critical for these effects chronic EtOH; however, molecular mechanisms leading augmented remain unclear. Using cell culture models vivo studies we demonstrate that EtOH increased transcription, which is independent NF-κB. reporter assays chromatin immunoprecipitation...
Objective It is known that noxious stimuli from inflamed tissue may increase the excitability of spinal dorsal horn neurons (a process as central sensitization), which can signal back and contribute to peripheral inflammation. However, underlying mechanisms have yet be fully defined. A number recent studies indicated NF‐κB/p65 involved in sensitization, well pain‐related behavior. Thus, aim this study was determine whether facilitate a inflammatory response rat adjuvant‐induced arthritis...
The identification of body fluids is important in forensic science.
Abstract A persistent challenge in utilizing gold nanostructures for surface‐enhanced Raman scattering (SERS) lies positioning analytes into the nanoscale hotspots that maximize SERS performance and achieve universal substrates. Here, a novel mesoporous nano‐pockets strategy is proposed to design high substrates by precisely capturing target molecules plasmonic hotspots. This achieved through silica as confined growth of short Au thorns on Fe 3 O 4 core (Fe @sAT@mSiO 2 ), whilst vertically...
The metabolism of etoposide was investigated by using human liver microsomes and nine recombinant cytochrome P450 (CYP) isoforms to identify the CYP isoform(s) involved in major metabolic pathway (3'-demethylation) as well evaluate possible interactions with several antitumor or supporting agents. 3'-demethylation followed a Michaelis-Menten one-enzyme kinetic behavior six microsomal samples. relationships were assessed different between activities for substrate probes respective isoforms,...
Abstract Medium-chain triglycerides (MCT), containing C 8 –C 12 fatty acids, are used to treat several pediatric disorders and widely consumed as a nutritional supplement. Here, we investigated the role of sirtuin deacylase Sirt5 in MCT metabolism by feeding knockout mice (Sirt5KO) high-fat diets either /C 10 acids or coconut oil, which is rich , for five weeks. Coconut but not feeding, induced periportal macrovesicular steatosis Sirt5KO mice. 14 C–C degradation was significantly reduced...
Hypochlorous acid (HClO), a reactive oxygen species, plays an essential role in the processes of physiology and pathology via reacting with most biological molecules. The abnormal level HClO may cause inflammation, especially arthritis. To further understand its key situ detection is necessary. Herein, water-soluble small molecule fluorescent probe (HDI-HClO) employed to monitor identify trace amounts system. In presence HClO, releases hydroxyl group emitting strong fluorescence because...
Studies using human liver microsomes and nine recombinant cytochrome P450 (CYP) isoforms (CYP1A1, 1A2, 2A6, 2B6, 2C9, 2C19, 2D6, 2E1 3A4) were performed to identify the CYP isoform(s) involved in major metabolic pathway (3-hydroxylation) of quinine humans. Eadie-Hofstee plots for formation 3-hydroxyquinine exhibited apparently monophasic behavior all 10 different microsomal samples studies. There was interindividual variability kinetic parameters, as follows: 1.8-, 3.2- 3.5-fold K(m) Vmax...
Tristetraprolin (TTP), an important immunosuppressive protein regulating mRNA decay through recognition of the AU-rich elements (AREs) within 3'-UTRs mRNAs, participates in pathogenesis liver diseases. However, whether TTP regulates stability other mechanisms remains poorly understood. Here, we report that was upregulated acute failure (ALF), resulting decreased stabilities CCL2 and CCL5 promotion N6-methyladenosine (m6A) methylation. Overexpression could markedly ameliorate hepatic injury...
Abstract Alcohol abuse has long been known to adversely affect innate immune responses and predispose infections. One cellular mechanism responsible for this effect is alcohol-induced suppression of TNF-α by mononuclear phagocytes. We undertook experiments better understand the mechanisms which alcohol dose-dependently suppresses TNF elaboration human monocytes. Here we show in primary monocytes cell lines that LPS-induced secretion post-transcriptionally inhibiting processing...
Ozone injection is generally used for the management of pain in diseases such as osteoarthritis (OA). Recent studies have shown that reduced autophagy chondrocytes plays an important role development OA. The purpose this study was to determine whether ozone treats OA by inducing chondrocytes.In study, primary were stimulated with IL-1β 24 hours simulate chondrocyte model, followed treatment (30 µg/ mL) or pretreatment 3-methyladenine compound C before treatment. Then, cell viability detected...
Aims The present study was designed to define the kinetic behaviour of sertraline N ‐demethylation in human liver microsomes and identify isoforms cytochrome P450 involved this metabolic pathway. Methods kinetics formation ‐demethylsertraline were determined from six genotyped CYP2C19 extensive (EM) three poor metabolisers (PM). Selective inhibitors specific monoclonal antibodies various also employed. Results N‐demethylsertraline all EM fitted by a two‐enzyme Michaelis‐Menten equation,...
To evaluate the safety and therapeutic efficacy of target percutaneous laser disc decompression (T-PLDD) for treatment lumbar herniation.PLDD using Nd:YAG has been regarded as an effective alternative herniation. However, all previous studies were concentrated on vaporizing nucleus pulposus in intervertebral space. We hypothesize that insertion needle into extruded part will decrease its volume provide superior clinical effects compared to therapies intradiscal pulposus.A total 25 patients...
Hepatic stellate cells (HSCs) are the main effectors for various types of hepatic fibrosis, including Schistosome-induced fibrosis. Multiple inflammatory cytokines/chemokines, such as transforming growth factor-β1 (TGF-β1), activate HSCs, and contribute to development MicroRNAs regulate gene expression at posttranscriptional level involved in regulation cytokine/chemokine synthesis. In this study, we showed that soluble egg antigen (SEA) stimulation Schistosoma japonicum infection...
Abstract 3D surface‐enhanced Raman scattering (SERS) platforms with multi‐dimensional hotspots and better tolerance to laser misfocus show great potential for the rapid detection of trace fentanyl. However, complex fabrication process fine structures limits wide‐ranging application SERS technique. Herein, a SERS‐active platform is fabricated utilizing scalable cost‐effective strategy that involves electroless deposition dense Au nanoparticles on natural butterfly wing scales (b‐wings@Au)....
The major metabolic pathway of quinine in the human has been shown to be 3-hydroxylation mediated mainly by cytochrome P450 (CYP) 3A4. In this extended vitro study, was further investigated using microsomes from mouse, rat, dog and livers compared among them terms enzyme-kinetic parameters quinine-drug interaction screenings. all species, 3-hydroxyquinine principal metabolite quinine. There intra- interspecies variability kinetic parameters, dogs exhibited a closer resemblance humans mean...
Dysregulated long noncoding RNAs (lncRNAs) are involved in the pathogenesis and development of human diseases, such as epithelial ovarian cancer (EOC). In this study, we identified EOC-related lncRNAs performed lncRNA mRNA microarray analyses using IOSE80, a normal ovary cell line, two carcinoma lines (SKOV3 SKOV3/DDP) to investigate potential roles EOC. lncRNA-HEIH expression EOC tissues was measured by quantitative real-time polymerase chain reaction (qPCR). addition, generated lncRNA-mRNA...