A5101400415- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Extracellular vesicles in disease
- Reproductive System and Pregnancy
- Endometrial and Cervical Cancer Treatments
- Endometriosis Research and Treatment
- RNA Research and Splicing
- Sarcoma Diagnosis and Treatment
- RNA modifications and cancer
- Cervical Cancer and HPV Research
- interferon and immune responses
- Cancer-related gene regulation
- Cell Adhesion Molecules Research
- Cytokine Signaling Pathways and Interactions
- Ubiquitin and proteasome pathways
- Orthopaedic implants and arthroplasty
- Epigenetics and DNA Methylation
- Immune cells in cancer
- CAR-T cell therapy research
- Cancer, Hypoxia, and Metabolism
- NF-κB Signaling Pathways
- Protein Tyrosine Phosphatases
- Immunotherapy and Immune Responses
- Cancer Research and Treatments
Fourth Affiliated Hospital of China Medical University
2015-2024
China Medical University
2015-2024
East China Normal University
2020-2024
Shanghai Changning Maternity and Infant Health Hospital
2015-2024
Chang'an University
2019
Anyang Hospital of Traditional Chinese Medicine
2019
MicroRNAs (miRNAs) are important regulators of many physiological and pathological processes, including cell proliferation, apoptosis, cycle arrest. In this study, we aimed to investigate the biological role miR-155 in cervical cancer underlying molecular mechanism involved tumorigenesis. The expression human tissues was detected by real-time PCR. MTT assay BrdU incorporation were used measure proliferation cells. Apoptosis cells distribution analyzed flow cytometry. We found that...
Long non-coding RNA HOTAIR has been revealed to participate in the tumorigenesis of various cancers. However, mechanism involvement cervical cancer not identified. Hence, this study aimed explore oncogenic and chemoresistant roles cancer, its underlying mechanism. RT-PCR, Western blot, Luciferase assay were employed determine relationship with miR-29b PTEN role cancer. CCK8 assay, cell migration, invasion used reveal proliferation metastasis. The inhibitory dose chemotherapeutics was...
MicroRNAs negatively regulate target gene expression at the post‐transcriptional level during carcinogenesis. Recent advances revealed that levels of several miRNAs are up‐ or down‐regulated in endometrial carcinoma (EC). Here we identify dysregulated EC and elucidate essential role let‐7a. The 86 was found to be different from adjacent normal tissues. Moreover, miR‐let‐7 members let‐7 highly associated with cancer. A functional investigation let‐7a suppressed proliferation HeLa cells by...
Emerging evidence suggests that physiological distress is highly correlated with cancer incidence and mortality. However, the mechanisms underlying psychological challenges-mediated tumor immune evasion are not systematically explored. Here, it demonstrated acute restraint (AR) increases level of plasma neuropeptide hormones, kisspeptin, expression levels its receptor, Gpr54, in hypothalamus, splenic tumor-infiltrating T cells, suggesting a correlation between neuroendocrine system...
To investigate the role of total cellular microRNA (miRNA) in regulating epithelial-to-mesenchymal transition (EMT) during human endometrial endometrioid adenocarcinoma (EEC). A miRCURY LNA array was used to evaluate miRNA profiles EEC tissues and corresponding nontumorous endometriums. An vitro model TGF-β induced EMT HEC-1-A cells miRNAs EMT. The expression SMAD3, SMAD5, a panel markers detected by Western blot quantitative PCR. results profiling endometriums demonstrated that miR-23a...
Cervical cancer stem cells contribute respond to considerable recurrence and metastasis of patients with cervical cancer. The stemness properties were partly regulated by the interaction lncRNAs miRNAs. HOTAIR functions as an oncogenic lncRNA. Previous research studies revealed its role in regulating various cancers. However, is still unknown. Here, cisplatin-resistant serum-free cultured exhibited properties. greater invasion migration compared their parental cells, which was similar...
Extracellular vesicles (EVs) are mediators between cancer cells and other types of cells, such as tumor-associated macrophages (TAMs), in the tumor microenvironment. EVs can remodel microenvironment regulate progression. However, underlying molecular mechanism these interactions remains unclear. First, we explored effect TAMs on survival prognosis patients with ovarian cancer. Next, isolated derived from (OV-EVs) through ultracentrifugation analyzed capacity OV-EVs to macrophage polarization...
S100P, a protein originally detected in the human placenta, has been found to play an important role development and invasion of tumors. Interestingly, we have recently discovered using data mining that S100P was considerably up-regulated during window implantation endometrium, but little further information available. Real-time PCR immunofluorescence were performed examine expression location endometrium endometrial cells. Estrogen progesterone added cultured cells test response sex...
Genotoxic agents remain the mainstay of cancer treatment. Unfortunately, clinical benefits are often countered by a rapid tumor adaptive response. Here, we report that oncoprotein B cell lymphoma 6 (BCL6) is core component confers solid resistance to genotoxic stress. Multiple promoted BCL6 transactivation, which was positively correlated with weakened therapeutic efficacy and worse outcome. Mechanistically, discovered treatment agent etoposide led transcriptional reprogramming multiple...
Metastasis is the main challenge of osteosarcoma treatment. Herein, we first reveal oncogenic role FAT10 in metastasis osteosarcoma. was upregulated osteosarcoma, especially metastatic High level associated with poorer prognosis patients. Moreover, Transwell and Matrigel assays revealed that silencing significantly inhibited invasive migratory abilities cells. assay vivo showed decreased number mice distant metastasis. We also found may act its functions through regulating HOXB9....
The aim of this study is to examine miRNA profiling and miR-369-3p participates in endometrioid adenocarcinoma (EEC) via the regulation autophagy. EEC its adjacent normal tissues were obtained from 20 clinical patients after surgery. MiRNA was performed using next generation sequencing (NGS) validated with quantitative real time PCR (qRT-PCR). qRT-PCR also employed measure autophagy-related protein 10 (ATG10) expression levels. Western blotting assay expressions ATG10 LC3B. Luciferase...
Cervical squamous cell carcinoma (CSCC) seriously affects women's health worldwide, and it is of great significance to illuminate the specific role circRNAs in CSCC.Three mRNA datasets, two miRNA datasets one circRNA dataset CSCC, downloaded from GEO, were utilized this study. Differentially expressed mRNAs (DEmRNAs), miRNAs (DEmiRNAs) (DEcircRNAs) identified, a ceRNA (DEcircRNA-DEmiRNA-DEmRNA) regulatory network was constructed. GO pathway analyses DEcircRNAs DEmRNAs performed. Quantitative...
Abstract Background The usefulness of transvaginal two-dimensional shear wave elastography (2D SWE) for cervical lesions is still uncertain. This study was to explore the value 2D SWE in evaluation stiffness normal cervix and its change with different factors under strict quality control (QC). Methods Two hundred patients were included this examined using quantitative evaluate QC. Results Intra-observer concordance parameters midsagittal planes acceptable intraclass correlation coefficients...