A5100420680- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Ovarian cancer diagnosis and treatment
- Ectopic Pregnancy Diagnosis and Management
- Chromatin Remodeling and Cancer
- Cancer Mechanisms and Therapy
- Assisted Reproductive Technology and Twin Pregnancy
- Nanoparticle-Based Drug Delivery
- Epigenetics and DNA Methylation
- Extracellular vesicles in disease
- Mesenchymal stem cell research
- Immune cells in cancer
- Cancer-related Molecular Pathways
- Cellular Mechanics and Interactions
- Proteoglycans and glycosaminoglycans research
- Galectins and Cancer Biology
- Cancer-related gene regulation
- Prenatal Screening and Diagnostics
Shanghai Medical College of Fudan University
2017-2020
Shanghai First People's Hospital
2017-2020
Nanjing Medical University
2017-2020
The present study aimed to identify shared microRNAs (miRNAs) in ovarian cancer (OC) cells and their exosomes using microarray data (accession number GSE103708) available from the Gene Expression Omnibus database, including exosomal samples 13 OC cell lines 3 normal surface epithelial lines, original samples. Differentially expressed miRNAs (DE‑miRNAs) were identified Linear Models for Microarray method, mRNA targets of DE‑miRNAs predicted miRWalk2 database. potential functions target genes...
MicroRNAs (miRNAs) serve important roles in drug‑resistance; however, exosomal miRNAs associated with drug‑resistance ovarian cancer (OC) have not been reported to date. The current study aimed analyze the drug resistance‑associated original OC cells and their derived exosomes using microarray data downloaded from Gene Expression Omnibus database (series GSE76449). chemosensitive cell lines SKOV3_ip1, A2780_PAR HEYA8, as well chemoresistant SKOV3_TR, A2780_CP20 HEYA8_MDR, were investigated....
A series of injectable and biocompatible delivery DOX-loaded supramolecular hydrogels were fabricated by using presynthesized DOX-2N-β-CD, Pluronic F-127 α-CD through host-guest interactions cooperative multivalent hydrogen bonding interactions. The compositions morphologies these confirmed PXRD SEM measurements. Moreover, the Rheological measurements studied studies found that they showed a unique thixotropic behavior, indicting fast self-healing property after continuous oscillatory shear...
The aim of this study is to examine miRNA profiling and miR-369-3p participates in endometrioid adenocarcinoma (EEC) via the regulation autophagy. EEC its adjacent normal tissues were obtained from 20 clinical patients after surgery. MiRNA was performed using next generation sequencing (NGS) validated with quantitative real time PCR (qRT-PCR). qRT-PCR also employed measure autophagy-related protein 10 (ATG10) expression levels. Western blotting assay expressions ATG10 LC3B. Luciferase...
Background and objectives: Most of previous studies are comparing the perinatal outcome on singleton babies. There has been no study evaluating possible effect embryo transfer outcomes in initial pregnancy resulting from fresh transfer. The purpose our is to access number obstetric risks order examine role single pregnancy.Materials methods: This a retrospective cohort analyzed 6439 women. 1647 patients were divided into one following three groups according number: group A (n = 94): (SET); B...
Cancer antigen 125 (CA125), encoded by the mucin 16 cell surface associated (MUC16) gene, has been widely used as a biomarker for ovarian cancer (OC) screening. However, it yet to be elucidated why its levels increase with tumor progression well certain other non‑malignant conditions. Based on our knowledge of inflammatory microenvironment (IME) in OC, HEY cells were treated several inflammation‑associated factors their antagonists, and was observed that upregulated MUC16 gene expression....
Mutations in the gene encoding AT-rich interactive domain 1A (ARID1A) are frequently observed endometrial cancer (EC) but molecular mechanisms linking genetic changes remain to be fully understood. The present study aimed elucidate influence of ARID1A mutations on signaling pathways. Missense, synonymous and nonsense heterozygous EC HEC‑1‑A cell line were verified by Sanger sequencing. Mutated small interfering RNA was transfected into cells. Biochemical microarray analysis revealed 13...
Abstract Aim To explore the involvement of Mad2 and BubR1 in cervical carcinogenesis. Methods The expressions tissues high‐grade squamous intraepithelial lesions (HSIL), low‐grade (LSIL) chronic cervicitis were analyzed immunohistochemistrily compared with those p16 INK4A . PEGFP‐Mad2 pEGFP‐BubR1 transfected into SiHa cells to overexpress Si‐RNAs knockdown. Cell viability was measured by cell counting kit‐8 (CCK‐8) assay. Migration invasion capabilities detected Transwell. Propidium iodide...
To observe the effects of human umbilical cord mesenchymal stem cells (UC-MSCs) on proliferation and apoptosis ovarian cancer cell SKOV3. Methods: Transwell co-culture was used to targeted homing effect UC-MSCs cells. MTT assay detect inhibitory conditioned medium SKOV3 proliferation, Annexin V-FITC/PI double staining apoptotic rate. Real-time PCR expression levels Ki-67, Bcl-2 Bax genes-relevant Results: in vitro. showed that significantly inhibited (P<0.01). rate 75% group higher than...