A5100698954- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Nanoparticle-Based Drug Delivery
- Endometrial and Cervical Cancer Treatments
- RNA Research and Splicing
- Ovarian cancer diagnosis and treatment
- Endometriosis Research and Treatment
- Cancer, Lipids, and Metabolism
- Cancer Cells and Metastasis
- Nanoplatforms for cancer theranostics
- Inflammasome and immune disorders
- Estrogen and related hormone effects
- Surgical Simulation and Training
- Advanced Drug Delivery Systems
- RNA Interference and Gene Delivery
- Cancer-related Molecular Pathways
- Minimally Invasive Surgical Techniques
- Innovations in Medical Education
- Anatomy and Medical Technology
- Epigenetics and DNA Methylation
- Lipid metabolism and disorders
- FOXO transcription factor regulation
- Galectins and Cancer Biology
- Wnt/β-catenin signaling in development and cancer
Shanghai Jiao Tong University
2016-2025
Shanghai First People's Hospital
2017-2024
International Peace Maternity & Child Health Hospital
2011-2016
Pyroptosis belongs to a novel inflammatory programmed cell death pathway, with the possible prognosis of endometrial cancer related terminal protein GSDMD. Hydrogen exerts biphasic effect on by promoting tumor and protecting normal cells, which might initiate GSDMD pathway-mediated pyroptosis.We performed immunohistochemical staining western immunoblotting analysis observe expression NLRP3, caspase-1, in human xenograft mice tissue lines. We investigated treatment hydrogen could boost ROS...
We previously identified TrkB as an oncogene involved in promoting metastasis endometrial carcinoma (EC). Here, we sought to delineate the effect of changes expression on global profile microRNAs (miRNAs) EC cells and further investigated correlation between certain miRNA clinicopathologic characteristics patients. Using quantitative reverse transcription-PCR (qRT-PCR), found that mRNA has no significant difference transcript levels normal endometrium captured by laser capture...
Abstract Epithelial ovarian cancer (EOC) is a highly lethal gynecological malignancy, and cisplatin resistance usually correlated with the poor prognosis of EOC. Increasing evidence indicates that dysregulation miRNAs related to chemotherapy sensitivity. In this study, we revealed miR-98-5p, member let-7 family, was enriched in cisplatin-resistant EOC cells compared cisplatin-sensitive cells, could promote cells. Further studies showed miR-98-5p directly target 3′-UTR Dicer1 suppress its...
Increasing evidence suggests that forkhead box A1 (FOXA1) is frequently dysregulated in many types of human cancers. However, the exact function and mechanism FOXA1 endometrial cancer (EC) remains unclear. expression, androgen receptor (AR) relationships these two markers with clinicopathological factors were determined by immunohistochemistry analysis. AR up-regulated transient transfection plasmids, down-regulated siRNA or short hairpin RNA (shRNA). The effects depletion overexpression on...
Mechanisms governing the metastasis of endometrial carcinoma (EC) are poorly defined. Recent data support a role for cell surface receptor tyrosine kinase TrkB in progression several human tumors. Here we present evidence direct EC. Immunohistochemical analysis revealed that and its secreted ligand, brain-derived neurotrophic factor (BDNF), more highly expressed EC than normal endometrium. High levels correlated with lymph node (p<0.05) lymphovascular space involvement Depletion by stable...
MicroRNAs play key roles in tumor proliferation and invasion. Here we show distinct expression of miR-222-3p between ERα-positive ERα-negative endometrial carcinoma (EC) cell lines primary tumors, investigation its relationship with ERα other clinical parameters. In vitro, the function was examined RL95-2 AN3CA lines. MiR-222-3p negatively correlated ERα. Over-expressed cells promoted proliferation, enhanced invasiveness induced a G1 to S phase shift cycle. Furthermore, inhibitor decreased...
// Yongli Zhang 1 , Wei Bao 2 Kai Wang Wen Lu Huihui Huan Tong 3 Xiaoping Wan Department of Obstetrics and Gynecology, Shanghai First Maternity Infant Hospital, Tongji University School Medicine, Shanghai, China International Peace & Child Health Hospital Affiliated to Jiao People's University, Correspondence to: Wan, email: wanxiaoping@tongji.edu.cn Keywords: SOX17, MAML3, endometrial cancer, β-catenin, Wnt signaling Received: January 10, 2016 Accepted: September 26, Published: October 12,...
Abstract Heterogeneity and drug resistance of tumor cells are the leading causes incurability poor survival for patients with recurrent breast cancer. In order to accurately deliver biological anticancer drugs different subtypes malignant omnidirectional targeted treatment cancer, a distinct design is demonstrated by embedding liposome‐based nanocomplexes containing pro‐apoptotic peptide survivin siRNA (LPR) into Herceptin/hyaluronic acid cross‐linked nanohydrogels (Herceptin‐HA) fabricate...
Objective EFEMP1, the epidermal growth factor–containing fibulin-like extracellular matrix protein 1, functions as an oncogene or a tumor suppressor depending on cancer types. In this study, we aim to determine whether EFEMP1 affects tumorigenesis and progression of endometrial carcinoma. Methods The expression was investigated using immunohistochemistry in panel normal endometrium (n = 40), atypical hyperplasia 10) carcinoma tissues 84). Methylation status promoter detected by...
The aberrant expression of miR107‑5p is closely related to the development several types human cancers. However, role miR‑107‑5p in endometrial carcinoma (EC) has not been fully confirmed. In present study, we aimed explore function EC carcinogenesis. samples and normal tissues were obtained by laser capture microdissection. It was determined that significantly higher than endometrium, advanced FIGO stages, lymph node metastasis myometrial invasion patients. Blocking inhibited cell...
A recent study reported that histone lysine specific demethylase 1 (LSD1, KDM1A) is overexpressed in endometrioid endometrial carcinoma (EEC) and associated with tumor progression as well poor prognosis. However, the physiological function mechanism of LSD1 cancer (EC) remains largely unknown. In this study, we demonstrate β-estradiol (E2) treatment increased expression via GPR30/PI3K/AKT pathway cells. Both siGPR30 PI3K inhibitor LY294002 block effect. RNAi-mediated silencing abolished...
We previously elucidated that long non-coding RNA Promoter of CDKN1A Antisense DNA damage Activated (PANDAR) as a p53-dependent oncogene to promote cisplatin resistance in ovarian cancer (OC). Intriguingly, high level p53-independent PANDAR was found cisplatin-resistant patients with p53 mutation. Here, our study probed the new roles and underlying mechanisms p53-mutant OC cisplatin-resistance.
The objectives of the study were to evaluate role mitogen-activated protein kinase (MAPK) signaling in normal, hyperplastic, and neoplastic endometrium relation estrogen receptor (ER) status investigate whether 17β-estradiol (E2) tamoxifen (TAM) mediate proliferation apoptosis endometrial cancer cells through MAPK pathway.The expressions phosphorylated total extracellular signal-regulated kinases 1/2 (phosphorylated [p-ERK1/2] ERK1/2 [t-ERK1/2]) analyzed with immunohistochemistry...
Studies have suggested that p16ink4a may be a surrogate biomarker for the diagnosis of cervical cancer; however, function in human cancer cells remains largely unknown. Therefore, this study, we aimed to investigate role cells. Immunocytochemistry was used examine invasive squamous cell carcinoma and its precancerous lesions. p16ink4a-siRNA transfected into SiHa HeLa deplete expression. The cellular levels mRNA protein were detected by qRT-PCR western blot analysis. Proliferation rates...
The relationship between endometrial carcinoma and cellular metabolism is unknown. In cancer, mutation rate of PTEN has been reported very high. Malate dehydrogenase 2 (MDH2) one the isoforms malate dehydrogenase, which involved in citric acid cycle mitochondria. Our study aimed to investigate role MDH2 played PTEN-regulated carcinoma.To reveal expression co-localization MDH2, immunohistochemistry immunofluorescent staining were used. Western blot, Real-time PCR, RNA interference...