The E2 protein encoded by human papillomaviruses (HPVs) inhibits expression of the viral E6 oncoprotein, which, in turn, regulates p53 target gene transcription. To identify cellular proteins involved E2-mediated transcriptional repression, we isolated an complex from cells conditionally expressing HPV-11 E2. Surprisingly, double bromodomain-containing Brd4, which is implicated cell cycle control and genome segregation, was found associated with conferred on ability to inhibit AP-1-dependent...

Post-translational modification can modulate protein conformation and alter binding partner recruitment within gene regulatory regions. Here, we report that bromodomain-containing 4 (BRD4), a transcription co-factor chromatin regulator, uses phosphorylation-induced switch mechanism to recruit E2 encoded by cancer-associated human papillomavirus (HPV) viral early cellular matrix metalloproteinase-9 (MMP-9) promoters. Enhanced MMP-9 expression, induced upon keratinocyte differentiation, occurs...

Background Closed incisional wound surgery frequently leaves dead space under the repaired skin, which results in delayed healing. The purpose of this study was to evaluate effect negative pressure therapy (NPWT) on wounds with after primary closure by evaluating fluid volume through suction drain, blood flow tensile strength, and histology wounds. Methods Bilateral 25-cm-long were created back 6 pigs partially removing muscle then suturing skin nylon sutures. NPWT (experimental group) or...

Metabolomics is useful for evaluating the fundamental mechanisms of improvements in health functions elderly. Additionally, gardening intervention as a regular physical activity elderly maintained and improved physical, psychology, cognitive, social health. This study was conducted to determine whether cognitive ability affected by participating program with metabolomic potential biomarker. The designed low moderate intensity Serum metabolites resulting from were subjected metabolite...

The present study aimed to assess the physical and psychological health benefits of a 15-session gardening intervention in elderly women investigate satisfaction intervention. Fifty (age >70 years) at two senior community centers located Seoul, South Korea, were selected participate this study. Twenty-four center “A” participated twice-weekly (≈50 minutes per session) during period Sept. Nov. 2015; 26 “B” comprised control group. At completion intervention, parameters such as body...

Brd4 is a chromatin adaptor containing tandem bromodomains binding to acetylated histone H3 and H4. Although has been implicated in the transcriptional control of papillomavirus-encoded E2 protein, it unclear how regulates function whether involvement transactivation transrepression common different types proteins. Using DNase I footprinting performed with vitro reconstituted human papillomavirus (HPV) nucleosome-free DNA templates, we found that facilitates its cognate sequences depending...

Significance With-no-lysine (K) 1 (WNK1) is widely expressed, yet little known about its actions beyond well-studied regulation of ion transport proteins. We find that WNK1 suppresses autophagy in multiple cell types, indicative outside transport. Because a complex cellular pathway with many regulatory inputs, identifying steps influenced by may also be value to better understand how controlled. Our results show connection between and PI3KC3 have implications for other processes mediated...

The objective of this study was to determine the effects gardening activities in senior individuals on brain nerve growth factors related cognitive function. Forty-one (age 76.6 ± 6.0 years) were recruited from local community Gwangjin-gu, Seoul, South Korea. A 20-min low-to-moderate intensity activity intervention, making a vegetable garden, performed by subjects garden plot located Konkuk University (Seoul, Korea) campus. involved six including cleaning plot, digging, fertilizing, raking,...

Activator protein-1 (AP-1) regulates diverse gene responses triggered by environmental cues and virus-induced cellular stress. Although many signaling events leading to AP-1 activation have been described, the fundamental features underlying binding site selection factor recruitment of dimeric complexes their target genes remain mostly uncharacterized. Using recombinant full-length human dimers formed between c-Jun Fos family members (c-Fos, FosB, Fra-1, Fra-2) for DNA transcriptional...

The objective of this study was to investigate the effects a gardening intervention as physical activity in women aged over 70 years. Twenty-one years were recruited from community Seoul, South Korea. Eleven subjects at senior center participated 15-session program (twice week, average 50 minutes per session) Sept. Nov. 2015. rest who another acted control group. Blood lipid profiles, blood pressure, inflammation peripheral-blood mononuclear cells (PBMC), and oxidative stress assessed by...

The objective of this study was to determine the exercise intensities 10 gardening tasks for men and women in their 20s. Fifteen university students [(mean ± SD) age 24.7 1.4 years body mass index 23.5 4.1 kg·m −2 ] participated study. On two occasions, subjects completed a high tunnel grassy area with weeds located near Cheongju, Chungbuk, South Korea. They performed five randomly ordered on each occasion. Subjects did task 5 minutes then sat rested chair before next task. Each subject wore...

Methods to improve osseointegration that include implantation of rhBMP-2 with various kinds carriers are currently considerable interest. The present study was conducted evaluate if the loaded β-TCP microsphere-hyaluronic acid-based powder-like hydrogel composite (powder gel) can act as an effective carrier for in host bone a defect or poor quality. release pattern then evaluated against rhBMP-2-loaded collagen sponge control group. Dental implants were also inserted into tibias three groups...

We report an action of the protein kinase WNK3 on neuronal mRNA splicing factor Fox-1. Fox-1 splices mRNAs encoding proteins important in synaptic transmission and membrane excitation. WNK3, implicated control excitability through actions ion transport, binds inhibits its activity a activity-dependent manner. Phosphorylation by does not change RNA binding capacity; instead, increases cytoplasmic localization Fox-1, thereby suppressing Fox-1–dependent splicing. These findings demonstrate role...

We designed a pilot study to develop family interaction model-integrated care farming program with mother-child pairs as the participants. In this study, we aimed assess effects of on communication skills and psychological health in families. Sixteen Sejong, South Korea participated study. The families once week for six weeks (90 min per session) between May July 2018. was developed based parenting education skills, strengths-based cognitive behavioral therapy, emotional intelligence model;...

Children in modern society are exposed to high levels of stress. Various previous studies have reported that horticultural activities help children's emotional stability. The objective this study is develop and implement a therapy program improve the intelligence, resilience, self-efficacy elementary school students.A total 582 Korean students (11-13 years old) from 28 schools participated study. consisted seven sessions was conducted once week for 60 min per session, during regular or...

The restricted environment in prison negatively affects psychological health of prisoners, which turn the rehabilitation prisoners. Previous studies have shown that horticultural activities were effective improving objectives to develop a therapy (HT) program and determine association 12 sessions with participants' using case analysis.Five cases who imprisoned at K correctional institution Gyeonggi-do, South Korea participated this study. They diagnosed as potential risk group health....

The objective of this study was to assess the physical and psychological effects an 18-session horticultural therapy (HT) program based on task-oriented training in stroke patients investigate patient satisfaction. HT consisted activities including motions such as reaching–grasping, squatting, stepping, stooping. A total 31 inpatients (16 males, 15 females) at B rehabilitation hospital Seongnam, South Korea, participated study. Fourteen a thrice weekly (6 weeks, ≈60 minutes per session)...