A5018803868- Protein Structure and Dynamics
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Amino Acid Enzymes and Metabolism
- Advanced NMR Techniques and Applications
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Electron Spin Resonance Studies
- Chemical Reactions and Isotopes
- Endoplasmic Reticulum Stress and Disease
- Wnt/β-catenin signaling in development and cancer
- Crystallization and Solubility Studies
- Redox biology and oxidative stress
- Metabolism and Genetic Disorders
- Estrogen and related hormone effects
- X-ray Diffraction in Crystallography
- Photosynthetic Processes and Mechanisms
- Hemoglobin structure and function
- Polyamine Metabolism and Applications
- Microbial Community Ecology and Physiology
- Biotin and Related Studies
- Machine Learning in Bioinformatics
- Glycosylation and Glycoproteins Research
University of Georgia
2008-2024
University of Kansas
2001-2007
Pacific Northwest National Laboratory
2003-2005
Georgia State University
2005
Duke University
2005
University of Oxford
2005
Harvard University
2004
Saint Louis University
2004
Rutgers, The State University of New Jersey
2003-2004
Cornell University
2002-2003
The use of random, fractional 13C-enrichment combined with low pass filtration has allowed the determination NMR relaxation parameters at an unprecedented number sites within recombinant human ubiquitin. Essentially complete 1H, 13C, and 15N resonance assignments for protein are reported. Carbon spin lattice heteronuclear NOE data have been analyzed in context Lipari−Szabo "model free" formalism. generalized order 56 main chain α C−H vectors determined found to correspond highly restricted...
The use of random, fractional 13C-enrichment combined with low pass filtration has allowed the determination NMR relaxation parameters at an unprecedented number sites within recombinant human ubiquitin. Essentially complete 1H, 13C, and 15N resonance assignments for protein are reported. Carbon spin lattice heteronuclear NOE data have been analyzed in context Lipari-Szabo model free formalism. generalized order 56 main chain alpha C-H vectors determined found to correspond highly restricted...
Research on the one-electron reduced analogue of NO, namely nitroxyl (HNO/NO(-)), has revealed distinguishing properties regarding its utility as a therapeutic. However, fleeting nature HNO requires design donor molecules. Metal nitrosyl (MNO) complexes could serve potential donors. The synthesis, spectroscopic/structural characterization, and {CoNO}(8) complex in pyrrole/imine ligand frame are reported. [Co(LN4(PhCl))(NO)] (1) does not react with established targets such Fe(III) hemes or...
Here we describe a protocol for purifying high yield αSyn proteinpurification the efficient production of monomers with lowpropensity self-aggregation. Alpha-synuclein seed amplification assays(αSyn-SAAs) have emerged as promising diagnostic tools Parkinson’s disease(PD) by detecting misfolded and amplifying signal through cyclicshaking resting in vitro. The ultra sensitivity assay affords theability to detect minute quantities peripheral tissues, but it alsopresents various technical...
Methionine oxidation in calmodulin (CaM) isolated from senescent brain results an inability to fully activate the plasma membrane (PM) Ca-ATPase, which may contribute observed increases cytosolic calcium levels under conditions of oxidative stress and biological aging. To identify functional importance Met144 Met145 near carboxyl-terminus CaM, we have used site-directed mutagenesis substitute leucines for methionines at other positions permitting site-specific Met145. Prior their oxidation,...
Ca2+, "a signal of life and death", controls numerous cellular processes through interactions with proteins. An effective approach to understanding the role Ca2+ is design a Ca2+-binding protein predicted structural functional properties. To de novo sites in proteins challenging due high coordination numbers incorporation charged ligand residues, addition Ca2+-induced conformational change. Here, we demonstrate successful site non-Ca2+-binding cell adhesion CD2. This designed protein,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructural Analysis of a Novel Interaction by Calmodulin: High-Affinity Binding Peptide in the Absence CalciumJeffrey L. Urbauer, James H. Short, Linda K. Dow, and A. Joshua WandCite this: Biochemistry 1995, 34, 25, 8099–8109Publication Date (Print):January 27, 1995Publication History Published online1 May 2002Published inissue 27 January 1995https://pubs.acs.org/doi/10.1021/bi00025a016https://doi.org/10.1021/bi00025a016research-articleACS...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTHigh-Resolution Triple-Resonance NMR Spectroscopy of a Novel Calmodulin·Peptide Complex at Kilobar PressuresJeffrey L. Urbauer, Mark R. Ehrhardt, Ramona J. Bieber, Peter F. Flynn, and A. Joshua WandView Author Information Departments Biological Sciences, Biophysical Chemistry, Center for Structural Biology State University New York Buffalo, 816 Natural Sciences Complex, 14260-3000 Cite this: Am. Chem. Soc. 1996, 118, 45,...
Carbon-13 isotope effects have been determined for all four carbons of l-malate as a substrate chicken liver malic enzyme, using either NADP or acetylpyridine−NADP the other substrate. The effect deuteration at C2 malate was then used to tell whether chemical mechanism this oxidative decarboxylation stepwise, with oxaloacetate an intermediate, concerted. With NADP, 13C C3 and C4 both decrease malate, showing stepwise mechanism, previously [Hermes, J. D., Roeske, C. A., O'Leary, M. H., &...
Reaction of carbene-stabilized disilicon (1) with the lithium-based dithiolene radical (2. ) affords first dianionic silicon tris(dithiolene) complex (3). Notably, formation 3 represents unprecedented utilization as a silicon-transfer agent. The nature was probed by multinuclear NMR spectroscopy, single-crystal X-ray diffraction, and DFT computations.
The structures of the bacterial RNA polymerase holoenzyme have provided detailed information about intersubunit interactions within holoenzyme. Functional analysis indicates that one these is critical in enabling to recognize major class promoters. It has been suggested this interaction, involving flap domain β subunit and conserved region 4 σ subunit, a potential target for regulation. Here we provide genetic biochemical evidence 4/β-flap interaction targeted by transcription factor AsiA....
Abstract Nature only samples a small fraction of the sequence space that can fold into stable proteins. Furthermore, structural variations in single fold, sometimes few amino acids, define protein’s molecular function. Hence, to design proteins with novel functionalities, such as recognition, methods control and sample shape diversity are necessary. To explore this space, we developed experimentally validated computational platform wide variety protein folds while sampling diversity. We...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEnergetics and dynamics of molecular recognition by calmodulinMark R. Ehrhardt, Jeffrey L. Urbauer, A. Joshua WandCite this: Biochemistry 1995, 34, 9, 2731–2738Publication Date (Print):March 7, 1995Publication History Published online1 May 2002Published inissue 7 March 1995https://pubs.acs.org/doi/10.1021/bi00009a001https://doi.org/10.1021/bi00009a001research-articleACS PublicationsRequest reuse permissionsArticle Views160Altmetric-Citations31LEARN...
Anti-sigma factors regulate prokaryotic gene expression through interactions with specific sigma factors. The bacteriophage T4 anti-sigma factor AsiA is a molecular switch that both inhibits transcription from bacterial promoters and phage early promotes at middle its interaction the primary of Escherichia coli , σ 70 . an all-helical, symmetric dimer in solution. solution structure reveals novel helical fold for protomer. Furthermore, protomer, surprisingly, contains helix–turn–helix DNA...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMultiple isotope effects with alternative dinucleotide substrates as a probe of the malic enzyme reactionPaul M. Weiss, Sandhya R. Gavva, Ben G. Harris, Jeffrey L. Urbauer, W. Cleland, and Paul F. CookCite this: Biochemistry 1991, 30, 23, 5755–5763Publication Date (Print):June 11, 1991Publication History Published online1 May 2002Published inissue 11 June 1991https://doi.org/10.1021/bi00237a018RIGHTS & PERMISSIONSArticle...
The C-terminus of calmodulin (CaM) functions as a sensor oxidative stress, with oxidation methionine 144 and 145 inducing nonproductive association the oxidized CaM plasma membrane Ca2+-ATPase (PMCA) other target proteins to downregulate cellular metabolism. To better understand structural underpinnings mechanism this switch, we have engineered mutant (CaM-L7) that permits site-specific M144 M145, used NMR spectroscopy identify changes in CaM-L7 interactions between CaM-binding sequence PMCA...
Cry11Ba produced by Bacillus thuringiensis subsp. jegathesan is an active toxin for larvae of the mosquito Anopheles gambiae. A 106-kDa aminopeptidase N (APN), called AgAPN2, was previously identified as a receptor in A. 70-kDa fragment AgAPN2 expressed Escherichia coli binds with high affinity (K(d) = 6.4 nM) and inhibits activity 98% bioassays [Zhang et al. (2008) Biochemistry 47, 11263-11272]. To identify regions involved toxicity, we truncated APN into peptides 28- 30-kDa ta tb,...
A systematic study was done examining the steady state kinetics of F1-catalyzed nucleotide hydrolysis in presence various activating divalent metal cations.Values K,,, and kcat were obtained from Lineweaver-Burk plots, kCat/K, values calculated.With some exceptions, kcat/Km shown to be independent present for ATP hydrolysis, hydrolyzed with magnesium as cation present, temperature most cations.An average value kcaJKm 2.62 X lo5 M" s" is calculated lower limit second order rate constant...