A5054449844- Spaceflight effects on biology
- High Altitude and Hypoxia
- Genetics, Aging, and Longevity in Model Organisms
- Heat shock proteins research
- Transgenic Plants and Applications
- Mitochondrial Function and Pathology
- Seed Germination and Physiology
- Bone health and osteoporosis research
- Plant responses to water stress
- Endoplasmic Reticulum Stress and Disease
- Plant Stress Responses and Tolerance
Università degli Studi della Tuscia
2017-2019
To reveal the integrative biochemical networks of wheat leaves in response to water deficient conditions, proteomics and metabolomics were applied two spring-wheat cultivars (Bahar, drought-susceptible; Kavir, drought-tolerant). Drought stress induced detrimental effects on Bahar leaf proteome, resulting a severe decrease total protein content, with impairments mainly photosynthetic proteins enzymes involved sugar nitrogen metabolism, as well capacity detoxifying harmful molecules. On...
The response of human primary osteoblasts exposed to simulated microgravity has been investigated and analysis metabolomic proteomic profiles demonstrated a prominent dysregulation mitochondrion homeostasis. Gravitational unloading treatment induced decrease in mitochondrial proteins, mainly affecting efficiency the respiratory chain. Metabolomic revealed that influenced several metabolic pathways; stimulating glycolysis pentose phosphate pathways, while Krebs cycle was interrupted at...
Decreased mechanical loading on bones, such as prolonged bed rest and microgravity during space flights, leads to the development of an osteoporotic-like phenotype. Although osteoblast hypo-functionality is reported be involved in progression bone pathological conditions, cellular mechanisms this process remain largely unknown. The combined application mass spectrometry "-omics" histochemical ultrastructural approaches have been employed investigate effects gravitational unloading human...
By proteomic, metabolomic and transcriptomic approaches we shed light on the molecular mechanism by which human keratinocytes undergo to terminal differentiation upon in vitro calcium treatment. Proteomic analysis revealed a selective induction of ribosomal proteins RSSA, an inhibitor cell proliferation inducer differentiation, HSP 60, protein folding chaperone GRP78, unfolding response signal. Additionally, observed EF1D, transcription factor for genes that contain heat-shock responsive...