A5088261186- Photosynthetic Processes and Mechanisms
- Erythrocyte Function and Pathophysiology
- Hemoglobin structure and function
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Mitochondrial Function and Pathology
- Blood transfusion and management
- Plant Stress Responses and Tolerance
- Meat and Animal Product Quality
- Blood properties and coagulation
- Heme Oxygenase-1 and Carbon Monoxide
- Blood groups and transfusion
- Neonatal Health and Biochemistry
- Photoreceptor and optogenetics research
- Cancer, Hypoxia, and Metabolism
- Hormonal and reproductive studies
- Endoplasmic Reticulum Stress and Disease
- Algal biology and biofuel production
- RNA modifications and cancer
- Protein Structure and Dynamics
- Biochemical effects in animals
- Adipose Tissue and Metabolism
- Identification and Quantification in Food
- Plant Micronutrient Interactions and Effects
Università degli Studi della Tuscia
2013-2023
Istituto Ortopedico Galeazzi
2019
Viterbo University
2015
Policlinico Umberto I
2013
Center for Systems Biology
2013
Digital Proteomics (United States)
2013
Defense Information Systems Agency
2006-2011
National Research Council
2009-2010
Istituto per il Sistema Produzione Animale in Ambiente Mediterraneo
2010
Saarland University
2003-2009
Highlights•Dendritic cells (DCs) can co-express Arg1 and IDO1 immunosuppressive enzymes•Arg1 activity is required for induction by TGF-β in DCs•Spermidine, a downstream product, but not arginine starvation, induces DCs•Arg1+ myeloid derived suppressor (MDSCs) render DCs via IDO1SummaryArginase 1 (Arg1) indoleamine 2,3-dioxygenase (IDO1) are immunoregulatory enzymes catalyzing the degradation of l-arginine l-tryptophan, respectively, resulting local amino acid deprivation. In addition, unlike...
Background Results from recent, highly debated, retrospective studies raised concerns and prompted considerations about further testing the quality of long stored red blood cells a biochemical standpoint.Design Methods We performed an integrated mass spectrometry-based metabolomics proteomics time-course investigation on SAGM-stored cells. In parallel, structural changes during storage were monitored through scanning electron microscopy.Results detected increased levels glycolytic...
Autism spectrum disorder (ASD) is still diagnosed through behavioral observation, due to a lack of laboratory biomarkers, which could greatly aid clinicians in providing earlier and more reliable diagnoses. Metabolomics on human biofluids provides sensitive tool identify metabolite profiles potentially usable as biomarkers for ASD. Initial metabolomic studies, analyzing urines plasma ASD control individuals, suggested that autistic patients may share some metabolic abnormalities, despite...
Nitric oxide (NO) has a fundamental role in the plant hypersensitive disease resistance response (HR), and S-nitrosylation is emerging as an important mechanism for transduction of its bioactivity. A key step toward elucidating mechanisms by which NO functions during HR identification proteins that are subjected to this PTM. By using proteomic approach involving 2-DE MS we characterized, first time, changes S-nitrosylated Arabidopsis thaliana undergoing HR. The 16 identified mostly enzymes...
The use of tetrahydrofuran/decanol as porogens for the fabrication micropellicular poly(styrene/divinylbenzene) monoliths enabled rapid and highly efficient separation peptides proteins by reversed-phase high-performance liquid chromatography (RP-HPLC). In contrast to conventional, granular, porous stationary phases, in which loading capacity is a function molecular mass, loadability both small large was within 0.4−0.9-pmol range 60- × 0.2-mm capillary column. Lower limits detection obtained...
In this study, we performed weekly assessment of morphology-related parameters through monitoring CPD-SAGM leuco-filtered erythrocyte concentrates from blood withdrawal until the 42nd day storage.Liquid storage red cells (RBCs) delivers a blood-derived therapeutic, which is safe, available, effective and affordable for most patients who need transfusion therapy in developed countries. However, growing body accumulating controversial evidences, either biochemical or retrospective clinical...
Two-dimensional gel electrophoresis and mass spectrometry were used to identify protein profile changes in red blood cell membranes stored over time under atmospheric oxygen, the presence or absence of protease inhibitors. New spots with lower molecular masses, ranging between 7 15 kDa observed during first days storage, while time, further fragments high-molecular-mass aggregates appeared, seen as a smearing upper part gel. Some turned out be shifts isoelectric point, consequence chemical...
The anti-tumoral effects of cannabinoids have been described in different tumor systems, including pancreatic adenocarcinoma, but their mechanism action remains unclear. We used specific for the CB1 (ACPA) and CB2 (GW) receptors metabolomic analyses to unravel potential pathways mediating cannabinoid-dependent inhibition cancer cell growth. Panc1 cells treated with show elevated AMPK activation induced by a ROS-dependent increase AMP/ATP ratio. ROS promote nuclear translocation GAPDH, which...
To improve the probability of detecting unintended side effects during maize gene manipulations by bombardment, proteomics was used as an analytical tool complementary to existing safety assessment techniques. Since seed proteome is highly dynamic, depending on species variability and environmental influence, we analyzed proteomic profiles one transgenic variety (event MON 810) in two subsequent generations (T05 T06) with their respective isogenic controls (WT05 WT06). Thus, comparing WT05...
The changes induced in the photosynthetic apparatus of spinach (Spinacia oleracea L.) seedlings exposed to iron deficiency shortly after germination were characterized with two proteomic approaches coupled chlorophyll and xanthophyll analysis vivo measurements photosynthesis. During first 10 d concentrations b violaxanthin greatly reduced, but all xanthophylls recovered 13–17 deficiency, when both chlorophylls negatively affected. No new protein was formed iron-deficient leaves, no...
Abstract Nitric oxide and reactive oxygen species play a key role in the plant hypersensitive disease resistance response, protein tyrosine nitration is emerging as an important mechanism of their co‐operative interaction. Up to now, proteins targeted by this post‐translational modification plants are still totally unknown. In study, we analyzed for first time undergoing during response analyzing via 1D‐ 2D‐western blot extracts from Arabidopsis thaliana challenged with avirulent bacterial...