A5025588603- Erythrocyte Function and Pathophysiology
- Metabolomics and Mass Spectrometry Studies
- Polar Research and Ecology
- Tryptophan and brain disorders
- Autism Spectrum Disorder Research
- Microbial Community Ecology and Physiology
- COVID-19 Clinical Research Studies
- Neonatal Health and Biochemistry
- Hemoglobin structure and function
- Hemophilia Treatment and Research
- Biocrusts and Microbial Ecology
- Microplastics and Plastic Pollution
- Child Nutrition and Feeding Issues
- SARS-CoV-2 and COVID-19 Research
- Long-Term Effects of COVID-19
- Photosynthetic Processes and Mechanisms
- Spaceflight effects on biology
- Meat and Animal Product Quality
- Plant Pathogens and Fungal Diseases
- Hormonal and reproductive studies
- Biochemical effects in animals
- Heme Oxygenase-1 and Carbon Monoxide
- Mycotoxins in Agriculture and Food
- Analytical Chemistry and Chromatography
- Macrophage Migration Inhibitory Factor
Università degli Studi della Tuscia
2014-2023
Viterbo University
2015
Highlights•Dendritic cells (DCs) can co-express Arg1 and IDO1 immunosuppressive enzymes•Arg1 activity is required for induction by TGF-β in DCs•Spermidine, a downstream product, but not arginine starvation, induces DCs•Arg1+ myeloid derived suppressor (MDSCs) render DCs via IDO1SummaryArginase 1 (Arg1) indoleamine 2,3-dioxygenase (IDO1) are immunoregulatory enzymes catalyzing the degradation of l-arginine l-tryptophan, respectively, resulting local amino acid deprivation. In addition, unlike...
Autism spectrum disorder (ASD) is still diagnosed through behavioral observation, due to a lack of laboratory biomarkers, which could greatly aid clinicians in providing earlier and more reliable diagnoses. Metabolomics on human biofluids provides sensitive tool identify metabolite profiles potentially usable as biomarkers for ASD. Initial metabolomic studies, analyzing urines plasma ASD control individuals, suggested that autistic patients may share some metabolic abnormalities, despite...
Among heavy metal stressors, cadmium (Cd) pollution is one leading threat to the environment. In this view, research efforts have been increasingly put forward promote individuation of phytoextractor plants that are capable accumulating and withstanding toxic metals, including Cd, in aerial parts. We hereby adopted hyperaccumulator B. juncea (Indian mustard) as a model investigate plant responses Cd stress at low (25 μM) high (100 doses. Analytical strategies included mass-spectrometry-based...
Oxygen dependent modulation of red blood cell metabolism is a long investigated issue. However, the recent introduction novel mass spectrometry-based approaches lends itself to implement our understanding effects prolonged exposure anaerobiosis. Indeed, most studies conducted so far have addressed short term issue, while limited body literature covering 42 days storage period only takes into account handful metabolic parameters (ATP, DPG, glucose, glyceraldehyde 3-phosphate, and lactate). We...
Recent advances in red blood cell metabolomics have paved the way for further improvements of storage solutions.In present study, we exploited a validated high performance liquid chromatography-mass spectrometry analytical workflow to determine effects vitamin C and N-acetylcysteine supplementation (anti-oxidants) on metabolome erythrocytes stored citrate-phosphate-dextrose saline-adenine-glucose-mannitol medium under bank conditions.We observed decreased energy metabolism fluxes (glycolysis...
Fusarium diseases, including head blight (FHB) and crown rot (FCR), reduce crop yield grain quality are major agricultural problems worldwide. These diseases also affect food safety through fungal production of hazardous mycotoxins. Among these, deoxynivalenol (DON) acts as a virulence factor during pathogenesis on wheat. The principal mechanism underlying plant tolerance to DON is glycosylation by specific uridine diphosphate–dependent glucosyltransferases (UGTs), which DON-3-β-d-glucoside...
Embryo assessment is currently performed through the analysis of morphology and cleavage rate. Recent studies have sought to identify a correlation between quali-quantitative profiles small molecules metabolic interest outcome embryo transfer. Approaches relying on both optical non-optical spectroscopy been proposed non-invasively monitor culture media. However, non-invasive approach only offers an indirect strategy embryos turn-around solution bypass limits detection these analytical...
Ischemia is a primary cause of neuronal death in retinal diseases and the somatostatin subtype receptor 2 agonist octreotide (OCT) known to decrease ischemia-induced cell death. Using recently optimized ex vivo mouse model ischemia, we tested anti-ischemic potential two additional neuropeptides, pituitary adenylate cyclase activating peptide (PACAP) substance P (SP), monitored major changes occurring at metabolic level. Metabolomics analyses were performed via fast HPLC online using...
In recent years, extracellular vesicles (EVs), cell-derived micro and nano-sized structures enclosed in a double-layer membrane, have been the spotlight for their high potential diagnostic therapeutic applications. Indeed, they act as signal mediators between cells and/or tissues through different mechanisms involving complex cargo exert number of biological effects depending upon EVs subtype cell source. Being produced by almost all types, are found every fluid including milk. Milk (MEVs)...
Autism Spectrum Disorder (ASD) encompasses a clinical spectrum of neurodevelopmental conditions that display significant heterogeneity in etiology, symptomatology, and severity. We previously compared 30 young children with idiopathic ASD unrelated typically-developing controls, detecting an imbalance several compounds belonging mainly to the metabolism purines, tryptophan other amino acids, as well derived from intestinal flora, reduced levels vitamins B6, B12 folic acid. The present study...
Recent advancements in the field of omics sciences have paved way for further expansion metabolomics. Originally tied to NMR spectroscopy, metabolomic disciplines are constantly and growingly involving HPLC mass spectrometry (MS)-based analytical strategies and, this context, we hereby propose a robust efficient extraction protocol metabolites from four different biological sources which subsequently analysed, identified quantified through high resolution reversed-phase fast spectrometry. To...
Abstract Male hypogonadism associated with insulin resistance (IR) very often leads to metabolic syndrome, at variance in its first stadium of sensitivity (IS). A plasma metabolomic investigation these patients can provide useful information comparison the values IS patients. To this aim from insulin-resistant males were analysed by using ultra high-performance liquid chromatography (UHPLC) and high-resolution mass spectrometry (HRMS). Thus, metabolites compared controls through multivariate...
During their passage through the circulation, red blood cells (RBCs) encounter severe physiological conditions consisting of mechanical stress, oxidative damage and fast changes in ionic osmotic conditions. In order to survive for 120 days, RBCs adapt surroundings by subtle regulation membrane organization metabolism. RBC homeostasis depends on interactions between integral protein band 3 with other cytoskeletal proteins, key enzymes various metabolic pathways. These are regulated binding...
Abstract Male hypogonadism is a disorder characterised by low levels of the hormone testosterone. At beginning subjects with testosterone do not show insulin resistance (insulin-sensitive patients), which develops over time (insulin-resistance patients). To analyse metabolic alterations mainly related to decreased testosterone, we performed metabolomics investigations on plasma males who showed normal levels. Plasma from patients (<8 nmol/l) and homeostatic model assessment for...
Recent physiological reports have documented how <italic>Cakile maritima Scop. Sea Rocket</italic> could accumulate high doses of Cd without altering its parameters.
Antarctic cryptoendolithic communities are self-supporting borderline ecosystems spreading across the extreme conditions of desert and represent predominant life-form in ice-free areas McMurdo Dry Valleys, accounted as closest terrestrial Martian analogue. Components these highly adapted extremophiles extreme-tolerant microorganisms, among most resistant known to date. Recently, studies investigated biodiversity community composition but metabolic activity metacommunity has never been...
Prompted by recent changes in climate trends, cropping areas and management practices, Fusarium head blight (FHB), a threatening disease of cereals worldwide, is also spreading unusual environments, where bread wheat (BW) durum (DW) are largely cultivated. The scarcity efficient resistance sources within adapted germplasm particularly alarming for DW, mainly utilized human consumption, which therefore at high risk kernel contamination health-dangerous mycotoxins (e.g. deoxynivalenol = DON)....
The identification of traces life beyond Earth (e.g., Mars, icy moons) is a challenging task because terrestrial chemical-based molecules may be destroyed by the harsh conditions experienced on extraterrestrial planetary surfaces. For this reason, studying effects biomolecules extremophilic microorganisms through astrobiological ground-based space simulation experiments significant to support interpretation data that will gained and collected during ongoing future exploration missions. Here,...
Abstract Evidence shows that individuals infected by SARS-CoV-2 experience an altered metabolic state in multiple organs. Metabolic activities are directly involved modulating immune responses against infectious diseases, yet our understanding of how host metabolism relates to inflammatory remains limited. To better elucidate the underlying biochemistry leukocyte response, we focused analysis on possible relationships between post-infection stages and distinct pathways. Indeed, observed a...