A5006695000- Erythrocyte Function and Pathophysiology
- Blood properties and coagulation
- Extracellular vesicles in disease
- Parasites and Host Interactions
- Lipid Membrane Structure and Behavior
- Neonatal Health and Biochemistry
- Blood groups and transfusion
- Parasite Biology and Host Interactions
- Complement system in diseases
- Research on Leishmaniasis Studies
- Global Maternal and Child Health
- T-cell and Retrovirus Studies
- Trypanosoma species research and implications
- Hemoglobinopathies and Related Disorders
- Exercise and Physiological Responses
- Adipokines, Inflammation, and Metabolic Diseases
- Vector-borne infectious diseases
Radboud University Nijmegen
2016-2020
Radboud University Medical Center
2016-2020
Radboud Institute for Molecular Life Sciences
2017-2020
University Medical Center
2019
Universidade Federal do Ceará
2018
Abstract Extracellular vesicles (EVs) are emerging as important mediators of cell–cell communication well potential disease biomarkers and drug delivery vehicles. However, the mechanical properties these largely unknown, processes leading to microvesicle‐shedding from plasma membrane not understood. Here an in depth atomic force microscopy spectroscopy study natural EVs is presented. It found that several different origin have a composition lipids proteins, but similar properties. generated...
During its 120 days sojourn in the circulation, red blood cell (RBC) remodels membrane. Acetylcholinesterase (AChE) is a glycosylphosphatidylinositol (GPI)-linked enzyme that may serve as marker for membrane processes occurring this ageing-associated remodelling process.Expression and enzymatic activity of AChE were determined on RBCs various ages, obtained by separation based volume density (ageing vivo), times storage bank conditions vitro), well RBC-derived vesicles.During ageing vivo,...
During their passage through the circulation, red blood cells (RBCs) encounter severe physiological conditions consisting of mechanical stress, oxidative damage and fast changes in ionic osmotic conditions. In order to survive for 120 days, RBCs adapt surroundings by subtle regulation membrane organization metabolism. RBC homeostasis depends on interactions between integral protein band 3 with other cytoskeletal proteins, key enzymes various metabolic pathways. These are regulated binding...
Microvesicle generation is an integral part of the aging process red blood cells in vivo and vitro. Extensive vesiculation impairs function survival after transfusion, microvesicles contribute to transfusion reactions. The triggers mechanisms microvesicle are largely unknown. In this study, we combined morphological, immunochemical, proteomic, lipidomic, metabolomic analyses obtain integrated understanding underlying during storage cell concentrates. Our data indicate that changes membrane...
A subset of the red blood cells (RBCs) patients with paroxysmal nocturnal hemoglobinuria (PNH) lacks GPI-anchored proteins. Some these proteins, such as CD59, inhibit complement activation and protect against complement-mediated lysis. This pathology thus provides possibility to explore involvement in cell homeostasis role proteins generation microvesicles (MVs) vivo. Detailed analysis morphology, volume, density various CD59 expression levels from PNH did not provide indications for a major...
Our knowledge on the molecular mechanisms of red blood cell aging is mostly derived from in vitro studies. The Four Days Marches Nijmegen Netherlands, world’s largest yearly walking event, constitutes a unique possibility to study effect mechanical and biochemical stressors occurring during moderate-intensity exercise vivo. Therefore, longitudinal measurements were performed biophysical, immunological, functional characteristics that are known change aging. data show induces generation...