A5027206976- Extracellular vesicles in disease
- Ferroptosis and cancer prognosis
- Heat shock proteins research
- Endoplasmic Reticulum Stress and Disease
- RNA modifications and cancer
- Erythrocyte Function and Pathophysiology
- Phagocytosis and Immune Regulation
- Lipid Membrane Structure and Behavior
- Complement system in diseases
- Autophagy in Disease and Therapy
- interferon and immune responses
- Immunotherapy and Immune Responses
- Mosquito-borne diseases and control
- Malaria Research and Control
- Cancer-related gene regulation
- Biochemical and Molecular Research
- RNA and protein synthesis mechanisms
- Cytomegalovirus and herpesvirus research
- melanin and skin pigmentation
- Circular RNAs in diseases
- Galectins and Cancer Biology
- Immune responses and vaccinations
- Research on Leishmaniasis Studies
- Polyamine Metabolism and Applications
- Blood properties and coagulation
Weizmann Institute of Science
2016-2021
Necroptosis is a regulated, nonapoptotic form of cell death initiated by receptor-interacting protein kinase-3 (RIPK3) and mixed lineage kinase domain-like (MLKL) proteins. It considered to be regulated necrosis, and, lacking the “find me” “eat signals that are feature apoptosis, necroptosis inflammatory. One such signal observed during apoptosis exposure phosphatidylserine (PS) on outer plasma membrane. Here, we demonstrate necroptotic cells also expose PS after phosphorylated kinase-like...
STING is an innate immune cytosolic adaptor for DNA sensors that engage malaria parasite (Plasmodium falciparum) or other pathogen DNA. As P. falciparum infects red blood cells and not leukocytes, how reaches such host in unclear. Here we show parasites inside can cell receptors from a distance by secreting extracellular vesicles (EV) containing parasitic small RNA genomic Upon internalization of DNA-harboring EVs human monocytes, released within the cytosol, leading to STING-dependent...
Metabolic changes within the cell and its niche affect fate are involved in many diseases disorders including cancer viral infections. Kaposi's sarcoma-associated herpesvirus (KSHV) is etiological agent of sarcoma (KS). KSHV latently infected cells express only a subset genes, mainly located latency-associated region, among them 12 microRNAs. Notably, these miRNAs responsible for inducing Warburg effect cells. Here we identify novel mechanism enabling to manipulate metabolic nature tumour...
Gastric cancer is the third most lethal worldwide, and evaluation of genomic status gastric cells has not translated into effective prognostic or therapeutic strategies. We therefore hypothesize that outcomes may depend on tumor microenvironment (TME), in particular, cancer-associated fibroblasts (CAF). However, very little known about role CAFs cancer. To address this, we mapped transcriptional landscape human stroma by microdissection RNA sequencing from patients with A stromal gene...
Mature red blood cells (RBCs) lack internal organelles and canonical defense mechanisms, making them both a fascinating host cell, in general, an intriguing choice for the deadly malaria parasite Plasmodium falciparum (Pf), particular. Pf, while growing inside its natural host, human RBC, secretes multipurpose extracellular vesicles (EVs), yet their influence on this essential cell remains unknown. Here we demonstrate that Pf parasites, cultured fresh donor blood, secrete within such EVs...
Extracellular vesicles facilitate cell-cell communication in the host-parasite interaction
Extracellular vesicles (EVs) shed by trypomastigote forms of Trypanosoma cruzi have the ability to interact with host tissues, increase invasion, and modulate innate response. In this study, EVs from T. or T.cruzi-infected macrophages were investigated as immunomodulatory agents during initial steps infection. Initially, scanning electron microscopy nanoparticle tracking analysis, we determined that cruzi-infected released higher numbers (50-300 nm) compared noninfected cells. Using...
Necroptosis is a regulated and inflammatory form of cell death. We, others, have previously reported that necroptotic cells release extracellular vesicles (EVs). We found EVs are loaded with proteins, including the phosphorylated key necroptosis-executing factor, mixed lineage kinase domain-like (MLKL). However, neither exact protein composition, nor impact, been delineated. To characterize their content, from untreated U937 were isolated analyzed by mass spectrometry-based proteomics. A...
Abstract Extracellular vesicles (EVs) are emerging as important mediators of cell–cell communication well potential disease biomarkers and drug delivery vehicles. However, the mechanical properties these largely unknown, processes leading to microvesicle‐shedding from plasma membrane not understood. Here an in depth atomic force microscopy spectroscopy study natural EVs is presented. It found that several different origin have a composition lipids proteins, but similar properties. generated...
Extracellular vesicles are essential for long distance cell-cell communication. They function as carriers of different compounds, including proteins, lipids and nucleic acids. Pathogens, like malaria parasites (Plasmodium falciparum, Pf), excel in employing vesicle release to mediate cell communication diverse processes, particularly manipulating the host response. Establishing research tools study interface between pathogen-derived their recipient cells will greatly benefit scientific...
Abstract Pathogens are thought to use host molecular cues control when initiate life-cycle transitions, but these signals mostly unknown, particularly for the parasitic disease malaria caused by Plasmodium falciparum . The chemokine CXCL10 is present at high levels in fatal cases of cerebral patients, reduced patients who survive and do not have complications. Here we show a Pf ‘decision-sensing-system’ controlled concentration. High expression prompts P. survival strategy via growth...
A large number of studies demonstrate that cell mechanics and pathology are intimately linked. In particular, deformability red blood cells (RBCs) is key to their function dramatically altered in the time course diseases such as anemia malaria. Due physiological importance mechanics, many methods for mechanical probing have been developed. While single-cell provide very valuable information, they often technically challenging lack high data throughput needed distinguish differences...
We recently reported that diadenosine tetraphosphate hydrolase (Ap(4)A hydrolase) plays a critical role in gene expression via regulation of intracellular Ap(4)A levels. This enzyme serves as component our newly described lysyl tRNA synthetase (LysRS)-Ap(4)A biochemical pathway is triggered upon immunological challenge. Here we explored the mechanism this enzyme's translocation into nucleus and found its immunologically dependent association with importin beta. Silencing beta prevented...
Abstract Necroptosis is a regulated and inflammatory form of cell death. We, others, have previously reported that necroptotic cells release extracellular vesicles (EVs). We found EVs are loaded with proteins, including the phosphorylated key necroptosis-executing factor, mixed lineage kinase domain-like (MLKL). However, neither exact protein composition, nor impact, been delineated. To characterize their content, from untreated U937 were isolated analyzed by mass spectrometry-based...
<p>This file contains Supplementary Materials and Methods, Figure Legends, 6 Figures with their legends</p>