A5075979511- Advanced Radiotherapy Techniques
- Sarcoma Diagnosis and Treatment
- Ferroptosis and cancer prognosis
- Cancer, Hypoxia, and Metabolism
- Immunotherapy and Immune Responses
- Molecular Biology Techniques and Applications
- Bioinformatics and Genomic Networks
- Glioma Diagnosis and Treatment
- Single-cell and spatial transcriptomics
- Radiomics and Machine Learning in Medical Imaging
- Phagocytosis and Immune Regulation
- Computational Drug Discovery Methods
- Extracellular vesicles in disease
- Cancer Genomics and Diagnostics
- Cancer, Stress, Anesthesia, and Immune Response
- Advanced Proteomics Techniques and Applications
- RNA modifications and cancer
- Cancer Research and Treatments
- Epigenetics and DNA Methylation
- vaccines and immunoinformatics approaches
- Genomics and Chromatin Dynamics
- Cancer-related Molecular Pathways
- Cancer Cells and Metastasis
- HIV/AIDS drug development and treatment
- Ubiquitin and proteasome pathways
Alzheimer's Association of Israel
2024-2025
Tel Aviv University
2019-2023
Glioblastoma (GBM) is the most aggressive form of glioma, with poor prognosis exhibited by patients, and a median survival time less than 2 years. We assemble cohort 87 GBM patients whose ranges from 3 months up to 10 years perform both high-resolution mass spectrometry proteomics RNA sequencing (RNA-seq). Integrative analysis protein expression, patient clinical information enables us identify specific immune, metabolic, developmental processes associated as well determine whether they are...
Necroptosis is a regulated and inflammatory form of cell death. We, others, have previously reported that necroptotic cells release extracellular vesicles (EVs). We found EVs are loaded with proteins, including the phosphorylated key necroptosis-executing factor, mixed lineage kinase domain-like (MLKL). However, neither exact protein composition, nor impact, been delineated. To characterize their content, from untreated U937 were isolated analyzed by mass spectrometry-based proteomics. A...
Exercise prevents cancer incidence and recurrence, yet the underlying mechanism behind this relationship remains mostly unknown. Here we report that exercise induces metabolic reprogramming of internal organs increases nutrient demand protects against metastatic colonization by limiting availability to tumor, generating an exercise-induced shield. Proteomic ex vivo capacity analyses murine revealed catabolic processes, glucose uptake, mitochondrial activity, GLUT expression. analysis...
Comprehensive molecular characterization of tumors aims to uncover cancer vulnerabilities, drug resistance mechanisms and biomarkers. Identification drivers was suggested as the basis for patient-tailored therapy, transcriptomic analyses were proposed reveal phenotypic outcome mutations. With maturation proteomic field, studies protein-RNA discrepancies that RNA are insufficient predict cellular functions. In this manuscript we discuss importance direct mRNA-protein comparisons in clinical...
Abstract Lysine histone acetyltransferase 6 (KAT6A/B) is a MYST family member of acetyltransferases (HATs), known to regulate gene expression by acetylation both and non-histone substrates. KAT6A/B promising cancer drug target, specifically in estrogen receptor positive (ER+) breast cancer, but anti-cancer activity other indications has also been shown, suggesting potential benefit for wider patient populations. Despite antitumor observed clinical trials, KAT6i inhibitors are reported cause...
Abstract Glioblastoma (GB) is the most aggressive neoplasm of brain. Poor prognosis mainly attributed to tumor heterogeneity, invasiveness and drug resistance. Only a small fraction GB patients survives longer than 24 months from time diagnosis (ie, long‐term survivors [LTS]). In our study, we aimed identify molecular markers associated with favorable as basis develop therapeutic applications improve patients' outcome. We have recently assembled proteogenomic dataset 87 clinical samples...
Abstract Necroptosis is a regulated and inflammatory form of cell death. We, others, have previously reported that necroptotic cells release extracellular vesicles (EVs). We found EVs are loaded with proteins, including the phosphorylated key necroptosis-executing factor, mixed lineage kinase domain-like (MLKL). However, neither exact protein composition, nor impact, been delineated. To characterize their content, from untreated U937 were isolated analyzed by mass spectrometry-based...
Summary Glioblastoma (GBM) is the most aggressive form of glioma, with poor prognosis exhibited by patients, and a median survival time less than two years. To examine survival-associated patterns, we assembled cohort 87 GBM patients whose ranges from 3 months up to 10 years, which are not bearing isocitrate-dehyderogenase (IDH)-1 mutation did undergo prior treatment. We integrated high-resolution mass-spectrometry proteomics RNA-sequencing yet unresolved proteomic contribution patient...
Abstract High-Grade Serous Ovarian Carcinoma (HGSOC) is a highly aggressive and heterogeneous cancer with variable treatment responses. Current strategies, including surgical resection chemotherapy, are often ineffective due to lack of molecular markers predict drug sensitivity patient prognosis. Mass spectrometry-based proteomics analysis powerful tool for biomarker discovery, but it restricted by the availability quality archived clinical specimens. In this study, we developed...
Abstract Ubiquitin-specific protease 1 (USP1) is a well-characterized deubiquitinating enzyme (DUB) that plays critical role in DNA damage repair (DDR). It was shown to regulate various key processes, most notably proliferating cell nuclear antigen (PCNA) the Translesion Repair (TLS) pathway and FANCD2/FANCI Fanconi Anemia (FA) pathway. Research indicates USP1 deficiency leads decrease survival disruption of genomic stability, suggesting inhibitors (USP1i) may hold promise treating DDR...
Abstract There are numerous drug development efforts of DNA damage response (DDR) targets, such as PARP, ATR, ATM, CHK1, where the ability to accurately identify responsive sub-populations for monotherapies and combinations is a major challenge. Patients routinely assigned these drugs based on BRCA mutation, or BRCAness profile, encompassing homologous recombination deficiencies (HRD) which do not always represent sub-populations. Since replication repair mechanisms dominated by signaling...
<h3>Background</h3> High-grade serous ovarian carcinoma (HGSOC) presents a significant clinical challenge, with five-year survival rate of only 30% and limited options for targeted therapy. Currently, PARP inhibitors provide benefit to approximately 10% patients BRCA mutations, but the overall effectiveness standard treatments, including surgery chemotherapy, remains low. A notable 70% experience chemoresistant relapse. The immunosuppressive tumor microenvironment (TME), influenced by both...
Abstract The translational gap of drug and biomarker discovery remains one the biggest challenges in pharmaceutical industry. On hand high throughput screening omics methods facilitate generation in-vitro in-vivo data dose-response for a particular or combinations. other hand, clinical techniques allow creation large scale treatment-naive datasets, with TCGA CPTAC as prominent examples. However, question which disease model best represents response/resistance mechanisms an oncology patient...
Abstract While poly-ADP-ribosylation (PAR) polymerase inhibitors (PARPi) are the only DNA damage repair (DDR) agent currently approved for clinical use, there numerous ongoing and preclinical drug development efforts of many other DDR-related targets, including ATR, ATM, CHK1 others. One major challenges these is ability to identify responsive populations assign right population or combination. Patients routinely assigned PARPi on-going DDRi trials based on presence BRCA mutation, BRCAness...
<div>Abstract<p>Exercise prevents cancer incidence and recurrence, yet the underlying mechanism behind this relationship remains mostly unknown. Here we report that exercise induces metabolic reprogramming of internal organs increases nutrient demand protects against metastatic colonization by limiting availability to tumor, generating an exercise-induced shield. Proteomic <i>ex vivo</i> capacity analyses murine revealed catabolic processes, glucose uptake,...
Supplementary Data from An Exercise-Induced Metabolic Shield in Distant Organs Blocks Cancer Progression and Metastatic Dissemination
Supplementary Data from An Exercise-Induced Metabolic Shield in Distant Organs Blocks Cancer Progression and Metastatic Dissemination