A5029148332- Autophagy in Disease and Therapy
- Mitochondrial Function and Pathology
- Quantum optics and atomic interactions
- Atomic and Subatomic Physics Research
- Quantum Information and Cryptography
- RNA and protein synthesis mechanisms
- Redox biology and oxidative stress
- DNA Repair Mechanisms
- Cytomegalovirus and herpesvirus research
- Plant Virus Research Studies
- Endoplasmic Reticulum Stress and Disease
- Herpesvirus Infections and Treatments
- Skin and Cellular Biology Research
- ATP Synthase and ATPases Research
- Advanced biosensing and bioanalysis techniques
- Cold Atom Physics and Bose-Einstein Condensates
- Photosynthetic Processes and Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Extracellular vesicles in disease
- Metalloenzymes and iron-sulfur proteins
- Cancer, Hypoxia, and Metabolism
- Enzyme Structure and Function
- MicroRNA in disease regulation
- Quantum Mechanics and Applications
- RNA Interference and Gene Delivery
University College London
2011-2024
Bar-Ilan University
2024
Hebrew University of Jerusalem
2007-2023
Weizmann Institute of Science
2022-2023
CRUK Lung Cancer Centre of Excellence
2017
Technion – Israel Institute of Technology
2015
Cancer Research UK
2011
Upon DNA damage, a cytosolic form of the mitochondrial enzyme fumarase moves into nucleus where, by virtue its enzymatic activity, it participates in cell's response to damage. This potentially explains known role as tumor suppressor.
Altered cell metabolism is inherently connected with pathological conditions including cancer and viral infections. Kaposi's sarcoma-associated herpesvirus (KSHV) the etiological agent of sarcoma (KS). KS tumour cells display features lymphatic endothelial differentiation in their vast majority are latently infected KSHV, while a small number lytically infected, producing virions. Latently express only subset genes, mainly located within latency-associated region, among them 12 microRNAs....
Metabolic changes within the cell and its niche affect fate are involved in many diseases disorders including cancer viral infections. Kaposi's sarcoma-associated herpesvirus (KSHV) is etiological agent of sarcoma (KS). KSHV latently infected cells express only a subset genes, mainly located latency-associated region, among them 12 microRNAs. Notably, these miRNAs responsible for inducing Warburg effect cells. Here we identify novel mechanism enabling to manipulate metabolic nature tumour...
The NEET family is a newly discovered group of proteins involved in diverse array biological processes, including autophagy, apoptosis, aging, diabetes, and reactive oxygen homeostasis. They form novel structure, the fold, which two protomers intertwine to two-domain motif, cap, unique redox-active labile 2Fe-2S cluster binding domain. To accelerate functional study proteins, as well examine whether they have an evolutionarily conserved role, we identified characterized plant protein. Here,...
Fumarase represents proteins that cannot be imported into mitochondria after the termination of translation (post-translationally). Utilizing mitochondrial and cytosolic versions tobacco etch virus (TEV) protease, we show mitochondrially targeted fumarase harboring a TEV protease recognition sequence is efficiently cleaved by but not protease. Nonetheless, was readily when its import slowed down either (i) disrupting activity TOM complex, (ii) lowering growth temperature, or (iii) reducing...
Efficient synchronization of single photons that are compatible with narrow band atomic transitions is an outstanding challenge, which could prove essential for photonic quantum information processing. Here we report on the independently generated using a room-temperature memory. The photon source and memory interconnected by fibers employ same ladder-level scheme. We store retrieve heralded end-to-end efficiency η_{e2e}=25% final antibunching g_{h}^{(2)}=0.023. Our process results in over...
The head-to-head oriented pair of melon resistance genes,
Abstract Coherent optical memories will likely play an important role in future quantum communication networks. Among the different platforms, based on ladder-type orbital transitions atomic gasses offer high bandwidth (>100 MHz), continuous (on-demand) readout, and low-noise operation. Here we report upgraded setup of our previously-reported fast ladder memory, with improved efficiency lifetime, reduced noise. The upgrade employs a stronger control field, wider signal beam, density,...
Dual localization of proteins in the cell has appeared recent years to be a more abundant phenomenon than previously reported. One mechanisms by which single translation product is distributed between two compartments, involves retrograde movement subset processed molecules back through organelle-membrane. Here, we investigated specific contribution mitochondrial targeting sequence (MTS), as cis element, distribution proteins, aconitase and fumarase. Whereas cytosolic presence fumarase...
Abstract The growing number of biological functions affected by autophagy ascribes a special significance to identification factors regulating it. activator protein-1 (AP-1) transcription are involved in most aspects cellular proliferation, death, or survival, yet no information regarding their involvement is available. Here, we show that the AP-1 proteins JunB and c-Jun, but not JunD, c-Fos, Fra-1, inhibit autophagy. inhibits induced starvation, overexpression short form ARF (smARF), potent...
Cellular redox status affects diverse cellular functions, including proliferation, protein homeostasis, and aging. Thus, individual differences in can give rise to distinct sub-populations even among cells with identical genetic backgrounds. Here, we have created a novel methodology track at single cell resolution using the redox-sensitive probe Grx1-roGFP2. Our method allows identification sorting of different oxidation levels either cytosol, mitochondria or peroxisomes. Using this...
Abstract Expanding the arsenal of prophylactic approaches against SARS-CoV-2 is utmost importance, specifically those strategies that are resistant to antigenic drift in Spike. Here, we conducted a screen over 16,000 RNAi triggers genome, using massively parallel assay identify hyper-potent siRNAs. We selected Ten candidates for vitro validation and found five siRNAs exhibited activity (IC50 < 20 pM) strong blockade infectivity live-virus experiments. further enhanced this by...
We suggest that clumpy dense outflowing equatorial rings around evolved giant stars, such as in supernova 1987A and the Necklace planetary nebula, are formed by bipolar jets compress gas towards plane. The launched from an accretion disc a stellar companion. Using flash hydrodynamics numerical code we perform 3D simulations, show expanding into spherical shell can plane lead to formation of ring. Rayleigh–Taylor instabilities interaction region break ring clumps. Under assumption same...
Dual targeting is an important and abundant phenomenon. Indeed, we estimate that more than a third of the yeast mitochondrial proteome dual localized. The enzyme fumarase highly conserved protein in all organisms with respect to its sequence, structure, enzymatic activity. In eukaryotes, it localized cytosol mitochondria. Saccharomyces cerevisiae, localization achieved by reverse translocation mechanism; molecules harbor sequence (MTS), are targeted mitochondria, begin their translocation,...
A recently discovered group of conditionally disordered chaperones share a very unique feature; they need to lose structure become active as chaperones. This activation mechanism makes these particularly suited respond protein-unfolding stress conditions, such oxidative unfolding. However, the role this disorder in stress-related activation, chaperone function, and crosstalk with other systems is not yet clear. Here, we focus on one members chaperones, thiol-redox switch bacterial...
The study of miRNAs and other noncoding RNAs has revolutionised our understanding gene expression regulation during cancer development progression, creating one the fastest-growing research fields in with realistic therapeutic potential. 2011 Non-coding Cancer Symposium hosted by University College London Institute focused on function oncogenesis.
Abstract Expanding the arsenal of prophylactic approaches against SARS-CoV-2 is utmost importance, specifically those strategies that are resistant to antigenic drift in Spike. Here, we conducted a screen with over 16,000 RNAi triggers genome using massively parallel assay identify hyper-potent siRNAs. We selected 10 candidates for vitro validation and found five siRNAs exhibited activity IC50<20pM strong neutralisation live virus experiments. further enhanced by combinatorial pairing...
Single-photon synchronization could prove essential for future quantum optical networks. We demonstrate of photon pairs with high rate and low noise using a room-temperature atomic memory compatible single-photon source.
Coherent optical memories will likely play an important role in future quantum communication networks. Among the different platforms, based on ladder-type orbital transitions atomic gasses offer high bandwidth ($>100$ MHz), continuous (on-demand) readout, and low-noise operation. Here we report upgraded setup of our previously-reported fast ladder memory, with improved efficiency lifetime, reduced noise. The upgrade employs a stronger control field, wider signal beam, density, higher depth,...
Varicella-zoster virus (VZV) is the etiological agent of chickenpox and shingles, diseases characterised by epidermal replication in skin mucosa formation blisters. We have previously shown that VZV infection has a profound effect on keratinocyte differentiation, altering normal pattern gene expression. In particular, reduces expression suprabasal keratins 1 10 desmosomal proteins, disrupting structure to promote blistering phenotype. Here, we extend these findings show upregulates keratin...