A5059774133- Genomics and Phylogenetic Studies
- Ethics in Clinical Research
- Genetic Associations and Epidemiology
- Forensic and Genetic Research
- Molecular Biology Techniques and Applications
- Genetic Mapping and Diversity in Plants and Animals
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- DNA and Biological Computing
- Genomics and Rare Diseases
- Biomedical Ethics and Regulation
- Single-cell and spatial transcriptomics
- Privacy-Preserving Technologies in Data
- SARS-CoV-2 and COVID-19 Research
- Epigenetics and DNA Methylation
- Genetics, Bioinformatics, and Biomedical Research
- Chromosomal and Genetic Variations
- Modular Robots and Swarm Intelligence
- RNA Research and Splicing
- Genetic diversity and population structure
- Genomic variations and chromosomal abnormalities
- Cancer Genomics and Diagnostics
- CRISPR and Genetic Engineering
- Law, AI, and Intellectual Property
- Genetic and phenotypic traits in livestock
Brigham and Women's Hospital
2024
Harvard University
2024
Reichman University
2021-2022
Misgav Ladach
2021
Vascular Biogenics (Israel)
2020
New York Genome Center
2015-2019
Columbia University
2015-2019
Center for Systems Biology
2018
Whitehead Institute for Biomedical Research
2011-2018
Harvard–MIT Division of Health Sciences and Technology
2016
Sharing sequencing data sets without identifiers has become a common practice in genomics. Here, we report that surnames can be recovered from personal genomes by profiling short tandem repeats on the Y chromosome (Y-STRs) and querying recreational genetic genealogy databases. We show combination of surname with other types metadata, such as age state, used to triangulate identity target. A key feature this technique is it entirely relies free, publicly accessible Internet resources....
A reliable and efficient DNA storage architecture has the potential to provide large-capacity information storage. However, current methods have only been able use a fraction of theoretical maximum. Erlich Zielinski present method, Fountain, which approaches maximum for stored per nucleotide. They demonstrated encoding information—including full computer operating system—into that could be retrieved at scale after multiple rounds polymerase chain reaction. Science , this issue p. 950
Consumer genomics databases have reached the scale of millions individuals. Recently, law enforcement authorities exploited some these to identify suspects via distant familial relatives. Using genomic data 1.28 million individuals tested with consumer genomics, we investigated power this technique. We project that about 60% searches for European descent will result in a third-cousin or closer match, which theoretically allows their identification using demographic identifiers. Moreover,...
Short tandem repeats (STRs) have a wide range of applications, including medical genetics, forensics, and genetic genealogy. High-throughput sequencing (HTS) has the potential to profile hundreds thousands STR loci. However, mainstream bioinformatics pipelines are inadequate for task. These treat mapping as gapped alignment, which results in cumbersome processing times biased sampling alleles. Here, we present lobSTR, novel method profiling STRs personal genomes. lobSTR harnesses concepts...
Short tandem repeats are among the most polymorphic loci in human genome. These play a role etiology of range genetic diseases and have been frequently utilized forensics, population genetics, genealogy. Despite this plethora applications, little is known about variation STRs population. Here, we report largest-scale analysis STR to date. We collected information for nearly 700,000 across more than 1000 individuals Phase 1 Genomes Project. Extensive quality controls show that reliable...
Family trees have vast applications in fields as diverse genetics, anthropology, and economics. However, the collection of extended family is tedious usually relies on resources with limited geographical scope complex data usage restrictions. We collected 86 million profiles from publicly available online shared by genealogy enthusiasts. After extensive cleaning validation, we obtained population-scale trees, including a single pedigree 13 individuals. leveraged to partition genetic...
microRNA (miRNA) expression profiles are often characteristic of specific cell types. The mouse mammary epithelial line, Comma-Dβ, contains a population self-renewing progenitor cells that can reconstitute the gland. We purified this and determined its miRNA signature. Several microRNAs, including miR-205 miR-22, highly expressed in cells, while others, let-7 miR-93, depleted. Let-7 sensors be used to prospectively enrich populations, enforced induces loss from mixed cultures.
Whole exome sequencing has become a pivotal methodology for rapid and cost-effective detection of pathogenic variations in Mendelian disorders. A major challenge this approach is determining the causative mutation from substantial number bystander that do not play any role disease etiology. Current strategies to analyze have mainly relied on genetic functional arguments such as mode inheritance, conservation, loss function prediction. Here, we demonstrate disease-network analysis provides an...
Next-generation sequencers have sufficient power to analyze simultaneously DNAs from many different specimens, a practice known as multiplexing. Such schemes rely on the ability associate each sequence read with specimen which it was derived. The current of appending molecular barcodes prior pooling is practical for parallel analysis up dozen samples. Here, we report strategy that permits simultaneous tens thousands specimens. Our approach relies use combinatorial strategies in pools rather...
Despite representing an important source of genetic variation, tandem repeats (TRs) remain poorly studied due to technical difficulties. We hypothesized that TRs can operate as expression (eQTLs) and methylation (mQTLs) quantitative trait loci. To test this we analyzed the effect variation at 4849 promoter-associated TRs, genotyped in 120 individuals, on neighboring gene DNA methylation. Polymorphic promoter were associated with increased variance local methylation, suggesting functional...
Current models of protecting human subjects create a zero-sum game privacy versus data utility. We propose shifting the paradigm to techniques that facilitate trust between researchers and participants.
Abstract One of the key questions regarding COVID19 vaccines is whether they can reduce viral shedding. To date, Israel vaccinated substantial parts adult population, which enables extracting real world signals. The vaccination rollout started on Dec 20th 2020, utilized mainly BNT162b2 vaccine, and focused individuals who are 60 years or older. By now, more than 75% this age group have been at least 14 days after first dose, compared to 25% between ages 40-60 old. Here, we traced Ct value...
In some organisms, small RNA pathways can act nonautonomously, with responses spreading from cell to cell. Dedicated intercellular delivery have not yet been characterized in mammals, although secretory compartments found contain RNA. Here we show that, upon contact, T cells acquire B RNAs that impact the expression of target genes recipient cells. Synthetic microRNA (miRNA) mimetics, viral miRNAs expressed by infected cells, and endogenous could all be transferred into These mechanisms may...