Endocrine-disrupting contaminants have been associated with aberrant changes in epigenetic pathways animals. In this study, zebrafish embryos were exposed bisphenol A (BPA) to search for associations between behavior and mechanisms fish. For concentration-dependent responses, a range of BPA concentrations (0.1 nM 30 μM). Embryos analyzed locomotor activity at 3-, 4-, 5-days post fertilization (dpf) response changing light conditions. Based on effects gene expression, 10 μM [from 24 96 hours...

Ascochyta blight, caused by the fungus lentis, is one of most destructive lentil diseases worldwide, resulting in over $16 million AUD annual loss Australia alone. The use resistant cultivars currently considered effective and environmentally sustainable strategy to control this disease. However, little known about genes molecular mechanisms underlying resistance against A. lentis. To uncover genetic basis differentially expressed were profiled plants during early stages lentis infection....

Abstract Farmed Atlantic salmon ( Salmo salar ) welfare and performance can be strongly influenced by stress episodes caused handling during farming practices. To better understand the changes occurring after an acute response, we exposed a group of parr to stressor, which involved netting transferring fish several new holding tanks. We describe time-course response sampling in groups before (T0) 10, 20, 30, 45, 60, 120, 240, 300, 330 min post-stress. A subgroup was also subjected same...

Micronutrients (vitamins and minerals) have been less well studied compared to macronutrients (fats, proteins, carbohydrates) although they play important roles in growth, metabolism, maintenance of tissues. Hence, there is growing interest understand the influence micronutrients across various aspects nutritional research. In last two decades, aquaculture feeds shifted containing more plant-based materials meet increasing demand maintain sustainability industry. A recent whole life cycle...

Abstract Although early embryonic protein production relies exclusively on maternal molecules such as messenger RNAs (mRNAs) incorporated into ovarian follicles, knowledge about any thermally induced, intergenerational effects is scarce in ectotherms. Here, we investigated how elevated temperatures (9°–12°C vs. 6°C) during oogenesis influenced the next generation by targeting mRNAs Atlantic cod (Gadus morhua) embryos, view of up- and down-regulated genes follicles pre-spawning adults....

Abstract Laboratory zebrafish are commonly infected with the intracellular, brain‐infecting microsporidian parasite Pseudoloma neurophilia . Chronic P. infections induce inflammation in meninges, brain and spinal cord, have been suggested to affect neural functions since clusters reside inside neurons. However, underlying immunological mechanisms associated infection not explored. Utilizing RNA‐sequencing analysis, we found that upregulated 175 downregulated 45 genes brain, compared...

Diet has been shown to influence epigenetic key players, such as DNA methylation, which can regulate the gene expression potential in both parents and offspring. Diets enriched omega-6 deficient omega-3 PUFAs (low dietary omega-3/omega-6 PUFA ratio), have associated with promotion of pathogenesis diseases humans other mammals. In this study, we investigated impact increased intake arachidonic acid (ARA), a physiologically important PUFA, on 2 generations zebrafish. Parental fish were fed...

The main aim of this study was to estimate the heritability for four measures deformity and their genetic associations with growth (body weight length), carcass (fillet yield) flesh-quality fat content) traits in yellowtail kingfish Seriola lalandi. observed major deformities included lower jaw, nasal erosion, deformed operculum skinny fish on 480 individuals from 22 families at Clean Seas Tuna Ltd. They were typically recorded as binary (presence or absence) analysed separately by both...

Abstract Astaxanthin (Ax), the main carotenoid responsible for distinct red flesh color in salmonids (Oncorhynchus, Salvelinus, Salmo, and Parahucho), is added to diet of farmed fish at a substantial cost. Despite great economical value salmon industry, key molecular mechanisms involved regulation muscle coloration are poorly understood. Chinook ( Oncorhynchus tshawytscha ) represent an ideal model study because they exhibit polymorphism two morphs, white pigmented fish. This was designed...

For a rapidly developing aquaculture industry where availability of raw materials for diet formulation are quickly changing it is fundamental to define what the dietary needs and benefits are. In farming Atlantic salmon (Salmo salar), highly unsaturated fatty acids (HUFA) known be essential minimum inclusion needed. The aim this study has been map performance midgut health towards HUFAs under normal challenging condition. post-smolt at 150 g were fed four experimental diets over feeding...

DNA methylation has an important role in intergenerational inheritance. An increasing number of studies have reported evidence germline inheritance induced by nutritional signals mammals. Vitamins and minerals as micronutrients contribute to growth performance vertebrates, including Atlantic salmon (Salmo salar), also a epigenetics environmental factors that alter status. It is understand whether the paternal diet can influence offspring through alterations signatures male germ cells.Here,...

It is well established that farmed Atlantic salmon (Salmo salar) need n3-highly unsaturated fatty acids (HUFA) in their diet to thrive and grow. However, the biological functions individual HUFAs may differ, implying future supplementation could require fixed ratios for maximum benefit. The intestinal barrier essential fish health, any disruption of can have detrimental effects. current experiment was designed examine response hindgut when fed a low HUFA with 8.5 g/kg EPA + DHA (4.5% total...

Disproportionate high intake of n-6 polyunsaturated fatty acids (PUFAs) in the diet is considered as a major human health concern. The present study examines changes hepatic gene expression pattern adult male zebrafish progeny associated with levels PUFA arachidonic acid (ARA) parental diet. generation (F0) was fed which either low (control) or ARA (high ARA). Progenies both groups (F1) were given control No differences body weight found between within stages F0 F1 generation. Few...

A moderate surplus of the one carbon (1C) nutrients methionine, folic acid, vitamin B6 and B12 above dietary recommendations for Atlantic salmon has shown to improve growth reduce hepatosomatic index in on-growing saltwater period when fed throughout smoltification. Metabolic properties molecular mechanisms determining improved are unexplored. Here, we investigate metabolic transcriptional signatures skeletal muscle taken before after smoltification acquire deeper insight into pathways...

Using functional feed ingredients to increase the intestinal health of Atlantic salmon (Salmo salar) could allow greater variation and higher inclusion plant-based proteins in feed. This is beneficial both for fish industry welfare. Components with strong bioactive properties, such as fucoidans from marine macroalgae, have a great potential ingredient. In present trial, we challenged post-smolts 17.8% soya HP48, diet that has been shown cause low-level inflammation. Fucoidan Undaria...

Moderate levels of intestinal damage and inflammation are often seen in intensive fish aquaculture. The causes may be due to antinutrients from plant meals, stress or other causes. There is currently a lack good models explore these effects so how mitigate the consequences. Most studies have used full-fat soy compounds that cause likely not reversible. In this study we explored possibility using soybean HP48, made solvent extracted peeled soybeans, as low-inflammation model post-smolt...

The intestinal epithelium is a selectively permeable barrier for nutrients, electrolytes and water, while maintaining effective protection against pathogens. Combinations of stressors throughout an animal's life, especially in agriculture aquaculture settings, may affect the regular operativity this organ with negative consequences animal welfare. In current study, we report effects three-week unpredictable chronic stress (UCS) period on morphology transcriptome response Atlantic salmon...

Gastropod molluscs are among the most abundant species that inhabit coral reef ecosystems. Many specialist predators, along with giant triton snail Charonia tritonis (Linnaeus, 1758) whose diet consists of Acanthaster planci (crown-of-thorns starfish), a corallivore known to consume enormous quantities reef-building coral. C. considered vulnerable due overexploitation, and decline in their populations is believed have contributed recurring A. population outbreaks. Aquaculture one approach...

Abstract Nephrocalcinosis is a common disorder in farmed Atlantic salmon, but the consequences for fish physiology are not well understood. We performed transcriptome study kidneys of salmon ( Salmo salar ) smolts without and with severe chronic nephrocalcinosis (NC). The revealed that numerous genes differentially expressed NC compared healthy salmon. most evident changes gene expression patterns group were massive downregulation metabolism energy production, upregulation signalling...

The identification of expression quantitative trait loci (eQTL) is an important component in efforts to understand how genetic variants influence disease risk. MicroRNAs (miRNAs) are short noncoding RNA molecules capable regulating the several genes simultaneously. Recently, novel isomers miRNAs (isomiRs) that differ slightly length and sequence composition compared their canonical have been reported. Here we present isomiR-eQTL, a user-friendly database designed help researchers find single...