A5070078338- Occupational and environmental lung diseases
- RNA modifications and cancer
- Genomic variations and chromosomal abnormalities
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cancer Genomics and Diagnostics
- Metastasis and carcinoma case studies
- Genetic factors in colorectal cancer
- Medical Imaging and Pathology Studies
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- TGF-β signaling in diseases
- Pleural and Pulmonary Diseases
- Caveolin-1 and cellular processes
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Cells and Metastasis
- Lung Cancer Treatments and Mutations
- Cancer-related gene regulation
- Ovarian cancer diagnosis and treatment
- MicroRNA in disease regulation
- Gene expression and cancer classification
- Metabolism, Diabetes, and Cancer
- Epilepsy research and treatment
- Pharmacogenetics and Drug Metabolism
- Signaling Pathways in Disease
- Ocular Oncology and Treatments
Finnish Institute of Occupational Health
2013-2022
University of Helsinki
2000-2011
Helsinki Institute for Information Technology
2011
Royal Brompton Hospital
2011
Aalto University
2011
Helsinki University Hospital
1999-2004
Finnish Foundation for Alcohol Studies
1998-1999
Lung cancer has the highest mortality rate of all cancers in world and asbestos-related lung is one leading occupational cancers. The identification molecular changes long been a topic increasing research interest. aim this study was to identify novel correlates by integrating miRNA expression profiling with previously obtained data (aCGH mRNA expression) from same patient material. performed on 26 tumor corresponding normal tissue samples highly asbestos-exposed non-exposed patients, eight...
Previous studies have revealed a robust association between exposure to asbestos and human lung cancer. Accumulating evidence has highlighted the role of epigenome deregulation in mechanism carcinogen‐induced malignancies. We examined impact on DNA methylation. Our genome‐wide (using Illumina HumanMethylation450K BeadChip) cancer tissue paired normal from 28 asbestos‐exposed or non‐exposed patients, mostly smokers, distinctive methylation changes. identified number differentially methylated...
To reveal genes relevant for malignant mesothelioma (MM), we carried out cDNA array experiments on 4 MM cell lines and 2 primary mesothelial cultures established from pleural fluid of non-cancer patients. Human cancer gene filters including 588 were used the experiments. Our study revealed 26 over-expressed that play a role in regulation fate, cycle, growth DNA damage repair 13 under-expressed encoding factors, receptors proteins involved adhesion, motility invasion to be common 3 or lines....
Abstract To identify new potential diagnostic markers for lung cancer, the expression profiles of 37 tumours were analysed using cDNA arrays. Seven samples from small‐cell cancer (SCLC), two large‐cell neuroendocrine (LCNEC), and 28 other non‐small‐cell cancers (mainly squamous cell adenocarcinoma). Principal component analysis permutation test used to detect differences in gene a set genes was found that distinguished high‐grade carcinomas (SCLC LCNEC) cancers. In addition, several genes,...
Malignant pleural mesothelioma (MM) is a rare tumour with high mortality, which can exhibit various morphologies classified as epithelioid, biphasic and sarcomatoid subtypes. To investigate the molecular changes in these tumours, we studied gene expression patterns by combined use of cDNA arrays tissue microarrays (TMA). Deregulation 588 cancer-related genes was screened 16 MM comprising all three subtypes compared references, i.e. normal mesothelial cell lines mesothelium. Array data were...
Deletion of the CDKN2A locus is centrally involved in development several malignancies. In malignant pleural mesothelioma (MPM), it one most frequently reported genomic alteration. MPM strongly associated with a patients' asbestos exposure. However, status and expression corresponding protein, p16, relation to patient's exposure poorly known. Copy number alterations 2p16, 9q33.1 19p13 have earlier been shown accumulate lung cancer but their unclear. We studied DNA copy numbers for using...
Exposure to asbestos is known induce lung cancer, and our previous studies have suggested that specific chromosomal regions, such as 19p13, are preferentially aberrant in tumours of asbestos-exposed patients. Here, we further examined the association between 19p region exposure using array comparative genomic hybridization fluorescence situ (FISH) FISH characterization asbestos-induced micronuclei (MN) human bronchial epithelial BEAS 2B cells vitro. We detected an increased number losses...
Five to seven percent of lung tumours are estimated occur because occupational asbestos exposure. Using cDNA microarrays, we have earlier detected exposure-related genomic regions in cancer. The region at 2p was one those that differed most between asbestos-exposed and non-exposed patients. Now, evaluated alterations 2p22.1-p16.1 as a possible marker for Lung from 205 patients with pulmonary fibre counts 0 570 million fibres per gram dry lung, were studied by fluorescence situ hybridisation...
Gut-derived endotoxins (lipopolysaccharide, LPS) complexed to LPS-binding protein (LBP) activate liver Kupffer cells via their CD14 receptor. Pro-inflammatory cytokines are released and this is postulated promote injury. We previously demonstrated enhanced expression of endotoxin receptor after 2 weeks alcohol administration. A similar result, based on 6 ethanol treatment, was recently reported suggested correlate with alcohol-induced To establish whether occurs prior or the initiation...
We have previously demonstrated an association between genomic alterations in 19p13, 2p16, and 9q33.1 asbestos exposure patients' lung tumours. This study detected allelic imbalance (AI) these regions asbestos-exposed cancer (LC) histologically normal pulmonary epithelium. extended the analyses of tumour tissue to cover a large LC patient cohort studied DNA copy number alteration (CNA) AI for first time combination. found both CNA ≥2/3 be significantly dose-dependently (P < 0.001) associated...
Genome-wide measurement of transcript levels is an ubiquitous tool in biomedical research. As experimental data continues to be deposited public databases, it becoming important develop search engines that enable the retrieval relevant studies given a query study. While systems based on meta-data already exist, data-driven approaches retrieve similarities expression itself have greater potential uncovering novel biological insights.We propose information method differential expression. Our...
Abstract Purpose: Asbestos causes DNA damage and the fibers, together with tobacco smoke, have a synergistic effect on lung cancer risk. We recently identified 18 chromosomal regions that showed differences in copy number between tumors of asbestos-exposed nonexposed patients. One previously asbestos-associated at 9q was further analyzed for allelic imbalance alterations (CNA) In addition, ploidy level studied. Experimental Design: Allelic 9q31.3-34.3 15 microsatellite markers 52 tumor...
Genetic alterations affecting 9p are commonly present in many cancer types and cancer‐related genes located this chromosomal region. We sequenced all of the a 32Mb region by targeted next generation sequencing (NGS) 96 patients with different types, including acute lymphoblastic leukemia, bone malignant fibrous histiocytoma/undifferentiated pleomorphic sarcoma, fibrosarcoma, Ewing's lung carcinoma. Copy number (CNA), mutations were studied from NGS data. detected deletion at CDKN2A locus as...
Diffuse malignant mesothelioma (DMM) of the pleura is a rare and aggressive disease, wherein long-term survival (LTS) rate low. The epithelioid subtype most prevalent form DMM with best prognosis. To study prognostic histopathologic factors associated extended in DMM, we examined 43 tumors from patients more than five years (LTSs) compared findings 84 reference group (RG) average survival. We analyzed considering previously published histopathological features attempted to identify...