A5049689071- Sarcoma Diagnosis and Treatment
- Polyomavirus and related diseases
- Antenna Design and Analysis
- Full-Duplex Wireless Communications
- Bone Tumor Diagnosis and Treatments
- Vascular Tumors and Angiosarcomas
- Cancer Genomics and Diagnostics
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Cardiac tumors and thrombi
- Cancer, Hypoxia, and Metabolism
- Oral and Maxillofacial Pathology
- Uterine Myomas and Treatments
- Lymphoma Diagnosis and Treatment
- Genomic variations and chromosomal abnormalities
- Musculoskeletal synovial abnormalities and treatments
- Management of metastatic bone disease
- Soft tissue tumor case studies
- Cancer Cells and Metastasis
- Ear and Head Tumors
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- RNA modifications and cancer
- Cancer and Skin Lesions
- Cancer-related molecular mechanisms research
University of Helsinki
2016-2025
Helsinki University Hospital
2015-2025
Hospital District of Helsinki and Uusimaa
2011-2017
Helios Klinikum Emil von Behring
2015
Helios Hospital Berlin-Buch
2015
Klinikum Stuttgart
2015
University of Turku
2012
Peijas Hospital
2009-2011
Finnish Cancer Registry
2009-2011
Institute for Molecular Medicine Finland
2009
Uterine leiomyomas, or fibroids, are benign tumors that affect millions of women worldwide and can cause considerable morbidity. To study the genetic basis this tumor type, we examined 18 uterine leiomyomas derived from 17 different patients by exome sequencing identified tumor-specific mutations in mediator complex subunit 12 (MED12) gene 10. Through analysis 207 additional tumors, determined MED12 is altered 70% (159 225) a total 80 patients. The Mediator 26-subunit transcriptional...
To explore the effect of high-dose ifosfamide in first-line treatment for patients < or = 40 years age with nonmetastatic osteosarcoma extremity.From March 1997 to September 2000, 182 were evaluated. Primary consisted two blocks (15 g/m2), methotrexate (12 cisplatin (120 mg/m2), and doxorubicin (75 mg/m2). Postoperatively, received cycles (90 three each ifosfamide, methotrexate, 150 Granulocyte colony-stimulating factor support was mandatory after ifosfamide/cisplatin/doxorubicin...
EURAMOS-1, an international randomized controlled trial, investigated maintenance therapy with pegylated interferon alfa-2b (IFN-α-2b) in patients whose osteosarcoma showed good histologic response (good response) to induction chemotherapy.
Merkel cell carcinoma is a rare malignancy of the skin. Integration polyomavirus (MCPyV) DNA to tumor genome frequent in these cancers. The clinical consequences MCPyV infection are unknown.We analyzed formalin-fixed paraffin-embedded tissue samples from 114 207 patients diagnosed with Finland 1979 2004 for presence use polymerase chain reaction (PCR), quantitative PCR, and sequencing examined associations between status histopathologic factors survival. median follow-up time after diagnosis...
Four international study groups undertook a large in resectable osteosarcoma, which included two randomised controlled trials, to determine the effect on survival of changing post-operative chemotherapy based histological response.Patients with osteosarcoma aged ≤40 years were treated MAP regimen, comprising pre-operatively 5-week cycles cisplatin 120 mg/m(2), doxorubicin 75 methotrexate 12 g/m(2) × 2 (MAP) and post-operatively further just MA. Patients after surgery. Those ≥10% viable...
Polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), Nasu-Hakola disease, is a globally distributed recessively inherited disease. PLOSL characterized by cystic bone lesions, osteoporotic features, and loss of white matter in the brain leading to spontaneous fractures profound presenile dementia. We have earlier molecular genetic background identifying mutations two genes, DAP12 TREM2. transmembrane adaptor protein that associates cell surface receptor The...
PURPOSE This study was performed to assess the prognostic value of proposed histopathologic method evaluate response primary tumor preoperative chemotherapy in Ewing's sarcoma. PATIENTS AND METHODS The evaluated from specimens 118 sarcoma patients, who were preoperatively treated by alone. Responses graded I III (macroscopic viable tumor, microscopic and no cells, respectively). Follow-up data available for all with a mean follow-up duration 86 months (range, 30 158). RESULTS A statistically...
Abstract Purpose: Merkel cell carcinoma (MCC) is rare skin cancer that often associated with polyomavirus (MCPyV) infection. Polyomaviruses repress tumor suppressor proteins, thus influencing cell-cycle progression, but the effect of MCPyV on key regulating proteins poorly understood. Experimental Design: We evaluated expression large T-antigen (LTA), Ki-67, and putative retinoblastoma protein (RB phospho-RB) p53, their regulatory (cyclin D1, cyclin E, p16, p21, p27, MDM2) by using...
Abstract Purpose: Merkel cell carcinoma (MCC) is a rare skin cancer that often harbors polyomavirus (MCPyV) DNA. The clinical importance of intratumoral immune cells and their associations with MCPyV infection are poorly understood. Experimental Design: We identified T lymphocytes (CD3-positive cells), T-cell subsets (CD4, CD8, FoxP3-positive natural killer (small CD16-positive macrophages (CD68 CD163-positive cells) in tumors 116 individuals diagnosed MCC Finland from 1979 to 2004 using...
Immunosuppression and Merkel-cell polyomavirus (MCPyV) infection may have a role in the pathogenesis of carcinoma (MCC), rare neuroendocrine skin. We studied incidence chronic lymphocytic leukaemia (CLL) MCC from files Finnish Cancer Registry largest hospital Finland, Helsinki University Central Hospital, 1979 to 2006. Presence MCPyV DNA MCCs was investigated by quantitative PCR. identified 4164 patients diagnosed with CLL 172 MCC. Six both diseases were found; first diagnosis four cases...
The aim of this study was to investigate the causes death in patients with burns using both medicolegal autopsy reports and clinical data collected during treatment specify irreversible organ dysfunctions leading death. Burn deaths occurring Helsinki Center from 1995 2005 were identified hospital database. charts retrieved compared. evaluated by plastic surgeons specialized burn care, an intensivist, a pathologist, special reference organ-specific changes reports. From 1999 2005, there 71...
Mediator complex participates in transcriptional regulation by connecting regulatory DNA sequences to the RNA polymerase II initiation complex. Recently, we discovered through exome sequencing that as many 70% of uterine leiomyomas harbour specific mutations exon 2 mediator subunit 12 (MED12). In this work, examined role MED12 other tumour types. The frequency was analysed altogether 1158 tumours direct sequencing. spectrum included mesenchymal (extrauterine leiomyomas, endometrial polyps,...
Microbial ecosystems that inhabit the human gut form central component of our physiology and metabolism, regulating modulating both health disease. Changes or disturbances in composition activity this microbiota can result altered immunity, inflammation, even cancer. To compare diversity stool samples from patient groups based on site neoplasm gastrointestinal tract (GIT) to assess possible contribution bacterial tumorigenesis. We studied by16S RNA gene sequencing DNA 83 patients, who were...
The c-myc and c-fos proto-oncogenes have several putative functions, including regulation of cell growth. In many neoplasms overexpression has been linked to poor prognosis. order study the role expression on tumorigenesis, metastatic spread osteosarcoma, frozen paraffin-embedded tissue 38 primary osteosarcoma 10 lung metastases were analyzed. mRNA analysis was performed by quantitative reverse transcription-polymerase chain reaction in situ hybridization. protein studied Western blot...
Leiomyosarcoma of nonvisceral soft tissues is an uncommon malignant tumor; thus, only small numbers cases have been reported. This study was based on a large series patients from the Scandinavian Sarcoma Group Register acquired during 15-year period (from 1986 to 2001). Follow-up information available for all patients.The authors analyzed clinical features 225 with cutaneous, subcutaneous, or deep-seated leiomyosarcoma extremities, trunk wall, and superficial parts head neck region determine...
Abstract Little is known about the genomic alterations underlying osteosarcoma. We performed a genomewide high‐resolution gene copy number analysis of 22 osteosarcoma samples using comparative hybridization on cDNA microarray that contained clones 13,000 genes. Nineteen cases had amplifications average spanned more than 1 Mb and 10 Numerous regions gain loss were identified, their boundaries defined at high resolution. Novel amplicons found 14q11, 17q25, 22q11–q13. Earlier‐known large...
Mediator regulates transcription by connecting gene-specific factors to the RNA polymerase II initiation complex. We recently discovered exome sequencing that specific exon 2 mutations in mediator complex subunit 12 (MED12) are extremely common uterine leiomyomas. Subsequent screening studies have focused on this mutational hot spot, and been detected leiomyosarcomas, extrauterine leiomyomas endometrial polyps, colorectal cancers. All missense changes or in-frame insertions/deletions. Here,...