A5060163981- Mesenchymal stem cell research
- Neurogenesis and neuroplasticity mechanisms
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Drug Transport and Resistance Mechanisms
- Cancer Cells and Metastasis
- Muscle Physiology and Disorders
- Electrospun Nanofibers in Biomedical Applications
- Liver Disease Diagnosis and Treatment
- Effects of Radiation Exposure
- Anesthesia and Neurotoxicity Research
- Liver physiology and pathology
- 3D Printing in Biomedical Research
- Nerve injury and regeneration
- Hematopoietic Stem Cell Transplantation
- Telomeres, Telomerase, and Senescence
- Genetic and Kidney Cyst Diseases
- Hedgehog Signaling Pathway Studies
- Biomedical Ethics and Regulation
- Cancer-related Molecular Pathways
- Reproductive Biology and Fertility
- Pediatric Hepatobiliary Diseases and Treatments
- Cancer Research and Treatments
- Renal and related cancers
- Organ Transplantation Techniques and Outcomes
Charles University
2014-2023
University of Hradec Králové
2007-2021
University Hospital Hradec Králové
2005-2017
Krajská Nemocnice Liberec
2014
Czech Academy of Sciences, Institute of Macromolecular Chemistry
2000
The lack of physiological parity between 2D cell culture and in vivo has led to the development more organotypic models, such as organoids. Organoid models have been developed for a number tissues, including liver. Current organoid protocols are characterized by reliance on extracellular matrices (ECMs), patterning culture, costly growth factors cellular diversity, structure, organization. hepatic generally simplistic composed hepatocytes or cholangiocytes, rendering them less...
Nestin is a type VI intermediate filament protein originally described in neural stem cells. Here we report that immature endothelial cells generated the course of angiogenesis express nestin. Endothelial embryonic capillaries destined to vascularize growing organs also this protein. Whereas nestin was sporadically expressed mature adult human nestin, consistently angiogenic vasculature. expression detected corpus luteum, which replenishes itself by angiogenesis. Nestin-immunoreactive...
Human prominin-1 (CD133) is expressed by various stem and progenitor cells originating from diverse sources. In addition to cells, its mouse ortholog in a broad range of adult epithelial where it selectively concentrated their apical domain. The lack detection human epithelia might be explained, at least part, the specificity widely used AC133 antibody, which recognizes an epitope that seems dependent on glycosylation. Here we decided re-examine expression tissues, particularly glandular...
We isolated and expanded stem cells from dental pulp extracted third molars using an innovative culture method consisting of low serum-containing medium supplemented with epidermal growth factor platelet-derived BB. evaluated the differentiation potential these when they were growing either adherently or as micromass/spheroid cultures in various media. Undifferentiated differentiated analyzed by flow cytometry, immunocytochemistry immunoblotting. The cytometry results showed that (DPSCs)...
Our aims were to isolate stem cells from human exfoliated deciduous teeth (SHED), cultivate them in vitro and investigate their basic biological properties, phenotype compare our findings with dental pulp (DPSC) isolated permanent teeth.Dental was gently evacuated teeth. After enzymatic dissociation of pulp, SHED cultivated modified cultivation media for mesenchymal adult progenitor containing 2% FCS supplemented growth factors insulin, transferrin, sodium (ITS) supplement. Cell viability...
AIMS: Our aims were to isolate dental pulp stem cells, cultivate them in various media and investigate their basic biological properties phenotype. METHODS: 16 lines of cells (DPSCs) isolated from an impacted third molar. After enzymatic dissociation pulp, DPSCs cultivated modified cultivation for mesenchymal adult progenitor containing 2 % or 10 fetal calf serum (FCS), FCS supplemented with ITS. Cell viability other examined periodically using a Vi-Cell analyzer Z2-Counter. DNA analysis...
Purpose: Mesenchymal stem cells isolated from bone marrow (BM-MSC) and periodontal ligament (PLSC) are with high proliferative potential ability to self-renewal. Characterization of these under genotoxic stress conditions contributes the assessment their prospective usage. The aim our study was evaluate changes in BM-MSC PLSC caused by ionizing radiation. Methods: Human were irradiated doses up 20 Gy Co60 observed 13 days; viability, proliferation, apoptosis senescence induction, expression...
Cholestatic liver diseases are characterized by intrahepatic accumulation of bile acids (BAs), exacerbating inflammation, and fibrosis. Dimethyl fumarate (DMF) is a clinically approved antiinflammatory drug that demonstrated protective effects in several experimental models injury. Still, its effect on BA homeostasis fibrosis has not been thoroughly studied. Herein, we hypothesized DMF could improve mitigate the progression cholestasis-induced The was administered to mice with...
Adult human dental pulp contains stem cells (DPSCs) that are capable of differentiation into osteoblasts, odontoblasts, adipocytes, and neuronal-like cells. Because these have potential use in tissue regeneration, herein we characterized the response DPSC lines to ionizing radiation (IR). These been developed from extracted molars healthy donors. DPSCs were cultivated a unique media supplemented with epidermal growth factor (EGF) platelet-derived (PDGF). Since homeostasis depends on precise...
Hepatic in vitro models that accurately replicate phenotypes and functionality of the human liver are needed for applications toxicology, pharmacology biomedicine. Notably, it has become clear function can only be sustained 3D culture systems at physiologically relevant cell densities. Additionally, drug metabolism drug-induced cellular toxicity often follow distinct spatial micropatterns metabolic zones acinus, calling capture this zonation. We demonstrate manufacture accurate...
Our previous findings performed in rat tissues demonstrated that intermediate filament nestin is expressed endothelial cells of newly formed blood vessels developing organs and neural transplants. The aim the present study was to identify other cellular markers nestin-positive (nestin+) vessels. To reach this goal we double immunofluorescent co-localize with endothelium-specific (CD31, CD34 II, vimentin) or perivascular (GFAP, SMA) paraffin-embedded sections normal human brain tissue, low-...
We provide a detailed characteristic of stem cells isolated and expanded from the human dental pulp. Dental pulp express mesenchymal cell markers STRO-1, vimentin, CD29, CD44, CD73, CD90, CD166, Sox2, nestin, nucleostemin. They are multipotent as shown by their osteogenic chondrogenic potential. measured relative telomere length in 11 lines at different passages quantitative real-time PCR. Despite large proliferative capacity, stable viability, phenotype, genotype over prolonged cultivation,...
Prominin-1 (CD133) is physiologically expressed at the apical membranes of secretory (serous and mucous) duct cells major salivary glands. We investigated its expression in various human gland lesions using two distinct anti-prominin-1 monoclonal antibodies (80B258 AC133) applied on paraffin-embedded sections characterized occurrence saliva. The 80B258 epitope was extensively adenoid cystic carcinoma, lesser extent acinic cell carcinoma pleomorphic adenoma, rarely mucoepidermoid carcinoma....
Seifrtová M, Havelek R, Ćmielová J, Jiroutová A, Soukup T, Brůčková L, Mokrý English D, Řezáčová M. The response of human ectomesenchymal dental pulp stem cells to cisplatin treatment. International Endodontic Journal , 45 401–412, 2012. Abstract Aim To determine the (DPSCs) DNA‐damaging cytostatic and compare it with normal dermal fibroblasts (HDFs). Methodology Dental were exposed 5, 10, 20 or 40 μmol L −1 cisplatin. proliferation affected was assessed by a Z2 Counter viability means...
The purpose of the present study was to compare activity two different clinically available iron chelators on development acute liver injury after administration bacterial endotoxin (lipopolysaccharide [LPS]) in rats. Lipopolysaccharide administered either alone or pretreatment with dexrazoxane (DEX) deferoxamine (DFO). Control groups received only saline its combination chelator. After 8 h, untreated LPS rats developed injury, signs inflammation and oxidative stress. reduced plasma...