Abstract Multidrug‐resistant human neuroblastoma cell lines obtained by selection with vincristine or actinomycin D from two independent clonal lines, SH‐SY5Y and MCIXC, have 3‐ to 30‐fold more surface epidermal growth factor (EGF) receptors than the drug‐sensitive parental cells as indicated EGF binding assays immunoprecipitation, affinity‐labeling, phosphorylation studies. Reversion drug sensitivity in one line was accompanied a return level of receptor. SH‐EP cells, clone derived same but...

The complete cDNA sequence of human intestine-specific plastin (I-plastin) was determined from a clone derived by PCR. It consists 97-bp 5' untranslated region, 1,887-bp coding and 1,655-bp 3' region. region predicts 629-residue polypeptide whose displays 86, 75, 73% identities with chicken intestine fimbrin, T-plastin, L-plastin, respectively. Recombinant I-plastin cross-linked actin filaments into bundles in the absence but not presence calcium. gene mapped PCR to chromosome 3; L-...

Aegistohadra species identified in recent surveys suggested that the genus shows high diversity and has wide occupancy southwest China. We introduce five new of genus, chuxiongensis Wu & Ding, sp. nov., A. huzhengkuni mengziensis menglunensis nov. sheni from Sichuan Yunnan, The distribution range China, geographically by microhabitats, is divided into northern half for ground-dwellers southern canopy-dwellers. Takasagohadra lini Hwang, Citation2013, a living tree trunk-dweller Guizhou,...

Straw had huge annual production, burning it in the air will cause serious pollution. Burning straw into biochar and returning to field not only make effective use of resources, but also improve soil quality. Different preparation conditions addition levels were used investigate ammonia nitrogen, nitrate total nitrogen contents leaching solution. The results showed that a favorable adsorption effect on cumulative reduced by 80.16% 2% 400°C treatment compared without adding biochar. final...

Many circuits in the mammalian brain are organized a topographic or columnar manner. These could be activated-in ways that reveal circuit function restore after disease-by an artificial stimulation system is capable of independently driving local groups neurons. Here we present simple custom microscope called ProjectorScope 1 incorporates off-the-shelf parts and liquid crystal display (LCD) projector to stimulate surface regions express channelrhodopsin-2 (ChR2). In principle, optogenetic...

Blue light (BL) at 405 nm and oregano essential oil (OEO) have shown bactericidal activity by its own. Here, we demonstrated that the two synergistically killed multidrug-resistant (MDR) Pseudomonas aeruginosa (Pa). Pa ATCC19660 HS0065 planktonic cells mature biofilms were reduced more than 7 log10 after treatment BL combined with OEO, in sharp contrast to no significant bacterial reduction monotreatment. The duo also sufficiently eliminated acute or biofilm-associated infection of open burn...

Central China harbors the native dart-sac-bearing camaenids Laeocathaica . The genus is revised and seven new species are proposed based on museum material newly collected specimens. This work confirmed that most have restricted habitats. comparison of dart sac apparatus among camaenid genera indicated importance presence proximal accessory might be analogous to membranous/muscular surrounding and/or distal region vagina near atrium, which also plays a significant role in diagnosis its...

Despite recent evidence suggesting that nerve transfer techniques help improve clinical outcomes, the underlying manner by which collateral-regenerated enters skeletal muscles to restore an organized pattern of neuromuscular junction (NMJ) is unclear. To construct animal models collateral regeneration, proximal peroneal was fixed distal tibial stump. Three months after surgery, spatial distribution motor endplates (MEPs) and corresponding in-muscle branches in long flexor digitorum were...

The global production of farmed Atlantic salmon amounts to over 2 million tons per year. Consumed all the world, is not only delicious but also nutritious. This paper deals with relationship between moisture content, low-field nuclear magnetic resonance (LF-NMR), scanning electron microscope (SEM), and sensory evaluation in baking process salmon. An artificial neural network (ANN) model has been established simulate change content energy consumed process. Through study LF-NMR, SEM,...

We report a new land snail species representing genus from the mountainous area of Zhejiang, China. The has depressed shell with granules all over surface. soft part taxon is characterized by presence mantle lobe whose form reviewed herein across wide range helicoid snails, developed epiphallic papilla, and absence penial sheath, dart sac apparatus flagellum. As indicated molecular-based phylogeny (16S + ITS2), deeply nested in eastern Asian camaenid genera shows close relationship camaenids...

The first complete mitochondrial genome of the dart sac-bearing camaenid Laeocathaica Möllendorff, 1899 was sequenced and analyzed in this study. whole mitogenome amdoana 14,660 bp length its nucleotide composition showed high AT-content 67.45%. It had 37 genes, including 13 protein-coding two ribosomal RNA 22 transfer genes. phylogeny yielded by both Bayesian inference maximum-likelihood method suggested that closely related to other camaenids with known genome. These genetic data are...

Abstract In northeastern China, successive years of cultivation have led to a decline in soil quality, process that is exacerbated by the over-application chemical fertilizers ensure staple food production. The large amount straw produced difficult effectively use recent years. There has been an increasing research on transforming into biomass char, but it often focused effects char addition physicochemical properties, without further exploring mechanisms this and its microorganisms....

Abstract JOURNAL/nrgr/04.03/01300535-202407000-00036/figure1/v/2023-11-20T171125Z/r/image-tiff Coordinated contraction of skeletal muscles relies on selective connections between the and multiple classes spinal motoneurons. However, current research spatial location motoneurons innervating different is limited. In this study, we investigated distribution relative position that control deep mouse hindlimbs, which were innervated by obturator nerve, femoral inferior gluteal peroneal tibial...

Abstract While early experience with moving stimuli is necessary for the development of direction selectivity in visual cortex carnivores, it unclear whether exerts a permissive or instructive influence. To test if specific parameters experienced could instructively sculpt emergent responses, visually naive ferrets were exposed to several hours unusual spatiotemporal patterns. In most immature ferrets, cortical neurons developed these patterns, indicating an animals that 1-10 days more...

摘要： 【摘要】 报道1例努南样综合征伴生长期毛发松动患者，检测分析其家系的基因突变情况。患儿女，3岁，特殊面容，身长矮小，手足掌纹多，皮肤颜色较深。头发稀疏细软，皮肤镜下可见黑点征，单一毛发毛囊单位，毛干直径粗细不等，直立发，新生毳毛，偶见发干缩窄、结节状发。采集先证者及其父母外周血，提取基因组DNA行全外显子组测序，结果显示，先证者SHOC2 基因2号外显子存在c.4A>G错义突变，导致第2号氨基酸由丝氨酸变为甘氨酸（p.S2G），患儿父母未检出该突变。结合临床表型与基因突变，确诊为努南样综合征伴生长期毛发松动。