Abstract The SARS‐CoV‐2 pandemic caused a public health crisis throughout the world and highlighted need for rapid sensitive testing as countermeasure. A specific biosensor platform is developed detection of antigen RNA SARS‐CoV‐2, its variant (B1.1.529). demonstrated combines unique protein catalyzed capture bioreceptors (PCCs) chimeric (RNA‐DNA) probe using LwaCas13a collateral cleavage activity atop graphene field effect transistors (gFETs). reported able to differentiate unprocessed 10 4...

Although fluid lipid films have been used widely in biosensing devices, they lack the high stability desired for technological implementation because noncovalent forces between constituent lipids are relatively weak. In this work, polymerized, planar supported bilayers ((poly)PSLBs) composed of diene-functionalized prepared and characterized. Several parameters relating (poly)PSLB structure to observations made studies polymerized bilayer vesicles were examined, including a comparison UV...

The cross-linking of supramolecular assemblies hydrated lipids is an effective method to stabilize these disruption by surfactants or aqueous alcohol. heterobifunctional lipids, Acryl/DenPC16,18 and Sorb/DenPC18,21, are examples a new class polymerizable lipid designed for the creation cross-linked structures. robust nature liposomes was demonstrated lyophilization followed their essentially complete redispersion in water. resulting were compared original sample quasi-elastic light...

Planar supported lipid bilayers (PSLBs) composed of phosphorylcholine (PC) lipids are known to be highly resistant nonspecific adsorption soluble proteins. However, these structures lack the stability desired for implementation in molecular devices (e.g., biosensors). In part 1 this report [Ross, E. E.; Spratt, T.; Liu, S.; Rozanski, L. J.; O'Brien, D. F.; Saavedra, S. Langmuir 2003, 19, 1752−1765], we described preparation and characterization (poly)PSLBs diene-functionalized lipids. Here...

Substrate-supported lipid bilayers have been prepared from bis-diene functionalized phosphorylcholine (PC) lipids and polymerized by UV irradiation. The overall bilayer structure is largely preserved upon removal water, although significant loss of material occurs the upper leaflet bilayer, likely due to desorption at air/water interface. morphology surface as observed AFM, indicate a substantially different arrangement in hydrated dehydrated states, presumably water near region. These...

Abstract A system for co-cultivation of anaerobic fungi with bacteria was established based on lactate cross-feeding to produce butyrate and butanol from plant biomass. Several co-culture formulations were assembled that consisted (Anaeromyces robustus, Neocallimastix californiae, or Caecomyces churrovis) the bacterium Clostridium acetobutylicum. Co-cultures grown simultaneously (e.g., ‘one pot’), compared cultures where cultured in fungal hydrolysate sequentially. Fungal hydrolysis...

The cross-linking polymerization of hydrated amphiphiles in monolayers, bilayers, and nonlamellar phases, i.e., bicontinuous cubic the inverted hexagonal is an effective method to modify their properties. Polymerization monomeric assembly proceeds a linear or manner depending on number location polymerizable groups per monomer. lipids with reactive each hydrophobic tail yields cross-linked polymers. Sisson et al. (1996) examined bilayers as function mole fraction bis-substituted...

Clostridium acetobutylicum fermentations are promising for production of commodity chemicals from heterogeneous biomass due to the wide range substrates organism can metabolize. Much work has been done elucidate pathways utilization aldoses, but little is known about metabolism more oxidized substrates. Two hexose derivatives, gluconate and galacturonate, present in low cost feedstocks, their will contribute overall metabolic output these substrates.A complete network glucose, gluconate,...

The cross-linking of supramolecular assemblies hydrated amphiphiles is an effective method to stabilize the assembly. A well-known strategy for lipids in lyotropic phases inclusion identical reactive groups each hydrophobic tail lipids. An alternative approach incorporate two similar but distinct into different locations within a single amphiphile. In principle, these heterobifunctional could react form ladderlike polymers or cross-linked polymers. This report describes synthesis,...

Abstract Data obtained from in situ Raman spectroscopy probes and high‐performance liquid chromatography (HPLC) analysis were applied together with chemometrics to build partial least squares models of metabolite concentrations for the industrially relevant organism Clostridium acetobutylicum . Models built predominant products (acetic acid, butyric butanol) C. cultures grown on glucose as a substrate. The then 3‐day culture real‐time, quantitative analysis. predicted outcomes new...

Clostridium acetobutylicum's metabolic pathways have been studied for decades due to its diversity and industrial value, yet many details of metabolism continue emerge. The flux through the recently discovered pentose phosphoketolase pathway (PKP) in C. acetobutylicum has determined growth on xylose but transcriptional analysis indicated may a greater contribution arabinose metabolism. To elucidate role xylulose-5-phosphate/fructose-6-phosphate (XFP), PKP acetobutylicum, experimental...

Clostridium acetobutylicum can ferment a wide variety of carbohydrates to the commodity chemicals acetone, butanol, and ethanol. Recent advances in genetic engineering have expanded chemical production repertoire C. using synthetic biology. Due its natural properties potential, this organism is promising candidate for converting biomass-derived feedstocks containing carbohydrate mixtures via or engineered pathways. Understanding how regulates metabolism during growth on imperative enable...

The coupling of optical fibers with Raman instrumentation has proven to be effective for real-time monitoring chemical reactions and fermentations when combined multivariate statistical data analysis. spectroscopy is relatively fast, little interference from the water peak present in fermentation media. Medical research explored this technique analysis mammalian cultures potential diagnosis some cancers. Other organisms studied via route include <i>Escherichia coli</i>, <i>Saccharomyces...

Clostridium acetobutylicum has traditionally been used for production of acetone, butanol, and ethanol (ABE). Butanol is a commodity chemical due in part to its suitability as biofuel; however, the current yield this product from biological systems not economically feasible an alternative fuel source. Understanding solvent phase physiology, tolerance, their genetic underpinning key future strain optimization bacterium. This study shows importance [NiFe]-hydrogenase physiology. C. genes...

The aim of this study was the reduction false arrhythmia alarms and improvement in accuracy calculated heart rates (HR) real-time patient monitors by fusion information from ECG SpO2 signals.These signals were analyzed independently to derive features for use analysis.Information regarding detection classification QRSs, signal quality index (SQI), HR obtained ECG.Pulses, quality, pulse rate (PR) hemodynamic parameters SpO2.Independent results each confirmed fusing both.When an alarm...

Abstract Clostridium acetobutylicum ATCC 824 effectively utilizes a wide range of substrates to produce commodity chemicals. When grown on different oxidation states, the organism exhibits recycling needs reduced intracellular electron carrying co-factors. Ratios with states were used modulate need balance carriers and demonstrate fine-tuned control metabolic output. Three oxidized first fed singularly, then in ratios three strains sp. Growth was most robust when glucose exclusive...

Evaluate the performance of encephalon state index (ESI) in depth anesthesia monitoring during clinical surgery, compared with bispectral (BIS).

Objective The patient monitors were used to explore the alarm actuality in a ICU and NICU investigate awareness reaction of medical staff alarms. Methods A series surveys interviews taken acquire clinicians' feelings attitudes monitoring researchers scheduled track alarms with annotations, collect data using central system. Results total 235 387 67 783 occurred respectively. average rate was about 142 alarms/patient-day 96 NICU. Conclusions There remains fatigue NICU, main reason is large...

Overview of integrated photonics sensors and peptides for the recognition streptavidin interleukin-6 in 10% fetal bovine serum.