A5100332696- Advanced Proteomics Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Cancer-related gene regulation
- Advanced biosensing and bioanalysis techniques
- Hemoglobinopathies and Related Disorders
- RNA modifications and cancer
- Gene expression and cancer classification
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
University of Cambridge
2020
Shanghai Jiao Tong University
2016
Abstract Direct tracing of small extracellular vesicle (sEV) cargoes holds unprecedented importance for elucidating the mechanisms involved in intercellular communication. However, high‐fidelity determination sEVs’ molecular situ has yet to be achieved due difficulty transporting probes into intact sEVs. Herein, a fLuorescent Intracellular‐Guided Hairpin‐Tetrahedron (fLIGHT) nanoprobe is described direct visualization sEV microRNAs situ. Integrating advantages nondestructive penetration via...
Abstract Protein microarray is a powerful technology for both basic research and clinical study. However, because there no database specifically tailored protein microarray, the majority of valuable original data still not publically accessible. To address this issue, we constructed Microarray Database (PMD), which designed archiving analyzing data. In PMD, users can easily browse search entire by experimental name, type sample information. Additionally, PMD integrates several analysis tools...
Abstract Systematic quantification of phosphoprotein within cell signaling networks in solid tissues remains challenging and precise large scale samples has great potential for biomarker identification validation. We developed a reverse phase protein array (RPPA) based phosphor-antibody characterization approach by taking advantage the lysis buffer compatible with alkaline phosphatase (AP) treatment that differs from conventional RPPA antibody validation procedure applied it onto fresh...
Abstract Background: Systematic quantification of phosphoprotein within cell signaling networks in solid tissues remains challenging and precise large scale samples has great potential for biomarker identification validation. Methods: We developed a reverse phase protein array (RPPA) based phosphor-antibody characterization workflow by taking advantage the lysis buffer compatible with alkaline phosphatase (AP) treatment here termed it as bottom-up antibody screening that differs from...