A5037799764- Radioactive element chemistry and processing
- CRISPR and Genetic Engineering
- Radioactive contamination and transfer
- Invertebrate Immune Response Mechanisms
- RNA and protein synthesis mechanisms
- Lanthanide and Transition Metal Complexes
- Radiation Dose and Imaging
- Algal biology and biofuel production
- Insect symbiosis and bacterial influences
- Stress Responses and Cortisol
- Developmental Biology and Gene Regulation
- Neuroscience and Neuropharmacology Research
- Hormonal Regulation and Hypertension
- Prenatal Screening and Diagnostics
- Alzheimer's disease research and treatments
- Aquaculture disease management and microbiota
- T-cell and B-cell Immunology
- Reproductive System and Pregnancy
- Animal Behavior and Reproduction
- Fungal and yeast genetics research
- Computational Drug Discovery Methods
- Marine Ecology and Invasive Species
- Biodiesel Production and Applications
- Biological Control of Invasive Species
- Neurobiology and Insect Physiology Research
Indiana University School of Medicine
2020-2025
Indiana University – Purdue University Indianapolis
2020-2025
Lawrence Berkeley National Laboratory
2012-2022
Berkeley College
2021
University of California, Berkeley
2012-2018
Integra (United States)
2015
University of California, San Francisco
2011-2013
Broad Center
2011-2013
University of Colorado Boulder
2008-2011
National Renewable Energy Laboratory
1993-2008
The amphipod crustacean Parhyale hawaiensis is a blossoming model system for studies of developmental mechanisms and more recently regeneration. We have sequenced the genome allowing annotation all key signaling pathways, transcription factors, non-coding RNAs that will enhance ongoing functional studies. member Malacostraca clade, which includes food crop species. analysed immunity related genes as an important comparative these species, where aquaculture problems increased farming has...
Two proteins, alpha 1 and pheromone/receptor transcription factor (PRTF), bind cooperatively to the upstream activation sequences (UAS) of yeast alpha-specific genes thereby activate their transcription. In these protein-DNA complexes, PRTF moiety interacts with a degenerate dyad symmetric sequence, P box. contributes also regulation second set cell-type-specific genes, a-specific genes. We used two in vitro assays show that is encoded, at least part, by MCM1 gene. one assay, truncated...
Transplantation of allogeneic stem cells into the early gestational fetus, a treatment termed in utero hematopoietic cell transplantation (IUHCTx), could potentially overcome limitations bone marrow transplants, including graft rejection and chronic immunosuppression required to prevent rejection. However, clinical use IUHCTx has been hampered by poor engraftment, possibly due host immune response against graft. Since fetal system is relatively immature, we hypothesized that maternal...
STE3 mRNA is present only in Saccharomyces cerevisiae alpha cells, not a or a/alpha and the transcript level increases about fivefold when cells are treated with a-factor mating pheromone. Deletions 5' noncoding region of defined 43-base-pair (bp) upstream activation sequence (UAS) that can impart both modes regulation to CYC1-lacZ fusion substituted for native CYC1 UAS. UAS activity required 1 product MAT alpha, which known be transcription alpha-specific genes. A chromosomal deletion...
Site-specific eukaryotic genome editing with CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) systems has quickly become a commonplace amongst researchers pursuing wide variety of biological questions. Users most often employ the Cas9 protein derived from Streptococcus pyogenes in complex an easily reprogrammed guide RNA (gRNA). These components are introduced into cells, and through base pairing complementary region double-stranded DNA (dsDNA)...
Background The interaction between genetics, epigenetics, and the environment plays a key role in development of alcohol use disorder (AUD). Pharmacological treatments targeting histone deacetylases (HDACs) suggest that HDAC inhibitors (HDACi) may be potential pharmacotherapeutic for AUD. objective current study was to test effects different HDACi on ethanol intake two rat lines selectively bred high ethanol-consumption. Method Adult naïve male drinking line 1 (HAD1) or alcohol-preferring...
Because of the continuing use nuclear fuel sources and heightened threats weapon use, amount produced released radionuclides is increasing daily, as risk larger human exposure to fission product actinides. A rodent model was used follow in vivo distribution representative actinides, administered free metal ions or complexed with chelating agents including diethylenetriamine pentaacetic acid (DTPA) hydroxypyridinonate ligands 3,4,3-LI(1,2-HOPO) 5-LIO(Me-3,2-HOPO). Different metabolic pathways...
Two hydroxypyridinone-containing actinide decorporation agents, 3,4,3-LI(1,2-HOPO) and 5-LIO(Me-3,2-HOPO), are being developed for the treatment of internal contamination by chelation therapy. Dose-response efficacy profiles in mice were established removal intravenously injected (238)Pu (241)Am after parenteral oral with these chelators. In both cases, presumed efficacious doses promoted substantially greater elimination rates than currently approved agent, diethylenetriamine-pentaacetic...
Abstract The photophysical properties, solution thermodynamics, and in vivo complex stabilities of Cm III complexes formed with multidentate hydroxypyridinonate ligands, 3,4,3‐LI(1,2‐HOPO) 5‐LIO(Me‐3,2‐HOPO), are reported. Both chelators were investigated for their ability to act as antenna chromophores , leading highly sensitized luminescence emission the metal upon complexation, long lifetimes (383 196 μs respectively) remarkable quantum yields (45 % 16 %, aqueous solution. bright peaks...
Although previous studies have examined the extent to which adrenocorticotropic hormone (ACTH) secretion depends on endogenous glucocorticoid levels, few parallel dependency of gene expression within corticotropin releasing (CRH) neuron containing subregion hypothalamic paraventricular nucleus (PVN). This study resting and stress-induced three immediate early genes (c-fos, zif268, NGFI-B mRNAs) two phenotypic restricted that code for ACTH secretagogues (CRH arginine vasopressin [AVP] hnRNAs)...
STE3 mRNA is present only in Saccharomyces cerevisiae alpha cells, not a or a/alpha and the transcript level increases about fivefold when cells are treated with a-factor mating pheromone. Deletions 5' noncoding region of defined 43-base-pair (bp) upstream activation sequence (UAS) that can impart both modes regulation to CYC1-lacZ fusion substituted for native CYC1 UAS. UAS activity required 1 product MAT alpha, which known be transcription alpha-specific genes. A chromosomal deletion...
Abstract Strategy, Management and Health Policy Enabling Technology, Genomics, Proteomics Preclinical Research Development Toxicology, Formulation Drug Delivery, Pharmacokinetics Clinical Phases I‐III Regulatory, Quality, Manufacturing Postmarketing Phase IV Internal radioactive contamination can arise in different ways, from accidents affecting nuclear sites industrial or medical sources, to the possible terrorist use of radiological dispersal devices. In all cases, internalized...
The negative-feedback actions of corticosterone (CORT) depend on both phasic and tonic CORT secretion patterns to regulate hypothalamic-pituitary-adrenal (HPA) axis activity. How these two different pattens influence specific intracellular signal transduction pathway activity within the cellular elements HPA has not been determined. For example, it is unknown whether suppressive over events medial parvocellular paraventricular nucleus (PVN) corticotrophin-releasing hormone (CRH) neurones,...
Transplutonium actinides are among the heaviest elements whose macroscale chemical properties can be experimentally tested.
Plutonium is a radioactive element widely used as an energy source and further employed in military applications. Although global stockpiles of plutonium have increased with time, the toxicological profile this metal has only been partially characterized. The vast majority studies focused on either biodistribution following internal contamination, or identification important proteins involved uptake within body. Thus, enhanced knowledge biology may improve future therapeutic approaches to...
Several clones encoding serine protease inhibitors were isolated from larval and adult flea cDNA expression libraries by immunoscreening PCR amplification. Each contained an open reading frame a protein of approximately 45 kDa, which had significant sequence similarity with the serpin family inhibitors. The thirteen to date encode proteins, share primary structure that includes nearly identical constant region about 360 amino acids, followed C-terminal variable 40-60 acids. sequences most...
Site-specific eukaryotic genome editing with CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated) systems has quickly become a commonplace amongst researchers pursuing wide variety of biological questions. Users most often employ the Cas9 protein derived from Streptococcus pyogenes in complex an easily reprogrammed guide RNA (gRNA). These components are introduced into cells, and through base pairing complementary region double-stranded DNA (dsDNA)...