A5078029133- Global Health Care Issues
- Anesthesia and Neurotoxicity Research
- Computational Drug Discovery Methods
- Chronic Obstructive Pulmonary Disease (COPD) Research
- 3D Printing in Biomedical Research
- GDF15 and Related Biomarkers
- RNA Research and Splicing
- Liver physiology and pathology
- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Drug-Induced Hepatotoxicity and Protection
- Organ Transplantation Techniques and Outcomes
University of California, San Francisco
2020-2024
The appearance of senescent cells in age-related diseases has spurred the search for compounds that can target tissues ("senolytics"). However, a major caveat with current senolytic screens is use cell lines as targets where senescence induced vitro, which does not necessarily reflect identity and function pathogenic vivo. Here, we developed new pipeline leveraging fluorescent murine reporter allows isolation quantification p16Ink4a+ diseased tissues. By high-throughput screening precision...
<title>Abstract</title> Tyrosine nitration alters the structure, function, and/or cellular localization of proteins1,2 and is implicated in pathology multiple diseases1-3. Although protein assumed to proceed via nonspecific chemical mechanisms, it highly selective, suggesting possibility enzymatic catalysis. Here, we showed that glyoxalase domain-containing 4 (GLOD4), a previously uncharacterized protein, an enzyme catalyzes selective nitration. A primary vivo target for GLOD4-mediated...
Abstract The liver is the primary organ responsible for drug detoxification. Drug‐induced injury (DILI) a leading cause of attrition during development and one main reasons that drugs are withdrawn from market. Hence, prevention DILI plays central role in overall drug‐discovery process. Most liver's energy supply comes form adenosine triphosphate (ATP), which largely generated by mitochondria. This article describes evaluation drug‐induced mitochondrial dysfunction using Seahorse...
Abstract Drug‐induced liver injury is an important cause of non‐approval in drug development and the withdrawal already approved drugs from market. Screening human hepatic cell lines for toxicity has been used extensively to predict drug‐induced preclinical development. Assessing hepatic‐cell health with more diverse markers will increase value vitro assays help mechanism toxicity. We describe three live cell‐based using HepG2 cells measure parameters indicative hepatotoxicity. The first...