A5041375987- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- Ion Transport and Channel Regulation
- Nuclear Structure and Function
- 3D Printing in Biomedical Research
- RNA Research and Splicing
- Computational Drug Discovery Methods
- Biomedical and Engineering Education
- Biosimilars and Bioanalytical Methods
- Ubiquitin and proteasome pathways
- Health and Medical Research Impacts
- Animal testing and alternatives
- Lung Cancer Research Studies
- Anesthesia and Neurotoxicity Research
- Diet, Metabolism, and Disease
- thermodynamics and calorimetric analyses
- Click Chemistry and Applications
- Protein Degradation and Inhibitors
- Biomedical Ethics and Regulation
- ATP Synthase and ATPases Research
- DNA Repair Mechanisms
- Gastrointestinal motility and disorders
- Mitochondrial Function and Pathology
- Toxin Mechanisms and Immunotoxins
- Fungal and yeast genetics research
National Institutes of Health
2004-2023
National Center for Advancing Translational Sciences
2004-2023
University of California, San Francisco
2018-2021
The Ohio State University
2010-2018
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2016
American University of Beirut
2003-2006
A single-step protein affinity purification protocol using Aspergillus nidulans is described. Detailed protocols for cell breakage, purification, and depending on the application, methods release from beads are provided. Examples defining utility of approaches, which should be widely applicable, included.
Transport through nuclear pore complexes (NPCs) during interphase is facilitated by the nucleoporin Nup2 via its importin α– and Ran-binding domains. However, Aspergillus nidulans vertebrate also locate to chromatin mitosis, suggestive of mitotic functions. In this study, we report that required for NPC inheritance in A. nidulans. Interestingly, role segregation independent domains but relies on a central targeting domain necessary localization viability. To test whether chromatin-associated...
Chromatin and nuclear pore complexes (NPCs) undergo dramatic changes during mitosis, which in vertebrates Aspergillus nidulans involves movement of Nup2 from NPCs to the chromatin region fulfill unknown functions. This transition is shown require Cdk1 mitotic kinase be promoted prematurely by ectopic expression NIMA kinase. localizes with a copurifying partner termed NupA, highly divergent yet essential NPC protein. NupA locate throughout prophase but anaphase move surround segregating DNA....
Abstract The liver is the primary organ responsible for drug detoxification. Drug‐induced injury (DILI) a leading cause of attrition during development and one main reasons that drugs are withdrawn from market. Hence, prevention DILI plays central role in overall drug‐discovery process. Most liver's energy supply comes form adenosine triphosphate (ATP), which largely generated by mitochondria. This article describes evaluation drug‐induced mitochondrial dysfunction using Seahorse...
Hypodiploid acute lymphoblastic leukemia (ALL) is an aggressive blood cancer with a poor prognosis despite intensive chemotherapy or stem cell transplant. Children and adolescents positive end-of-induction minimal residual disease have overall survival lower than 30%. However, data regarding therapeutic alternatives for this nearly nonexistent, emphasizing the critical need new adjunctive therapies that can improve outcomes. We previously reported on efficacy of venetoclax (ABT-199) in...
Abstract The National Center for Advancing Translational Sciences (NCATS) Assay Guidance Manual (AGM) Workshop on 3D Tissue Models Antiviral Drug Development, held virtually 7–8 June 2022, provided comprehensive coverage of critical concepts intended to help scientists establish robust, reproducible, and scalable tissue models study viruses with pandemic potential. This workshop was organized by NCATS, the Institute Allergy Infectious Diseases, Bill Melinda Gates Foundation. During workshop,...
The nuclear pore complex (NPC) protein Nup2 plays interphase transport roles and in Aspergillus nidulans also functions to bridge NPCs at mitotic chromatin for their faithful coinheritance daughter G1 nuclei. In this study, we further investigate the of A. nidulans. Although is not required import all proteins after mitosis, it normal accumulation NPC basket-associated components Mad2 Mlp1 as well THO Tho2. Targeting nuclei partially rescues delay seen nup2 mutants indicating that some...
Most solid tumors are aneuploid, carrying an abnormal number of chromosomes, and they frequently missegregate whole chromosomes in a phenomenon termed chromosome instability (CIN). While CIN can be provoked through disruption numerous mitotic pathways, it is not clear which these mechanisms most critical, or whether alternative could also contribute significantly vivo. One difficulty determining the relative importance candidate regulators has been lack straightforward, quantitative assay...
Abstract Drug‐induced liver injury is an important cause of non‐approval in drug development and the withdrawal already approved drugs from market. Screening human hepatic cell lines for toxicity has been used extensively to predict drug‐induced preclinical development. Assessing hepatic‐cell health with more diverse markers will increase value vitro assays help mechanism toxicity. We describe three live cell‐based using HepG2 cells measure parameters indicative hepatotoxicity. The first...
Abstract Most solid tumors are aneuploid, carrying an abnormal number of chromosomes, and they missegregate whole chromosomes in a phenomenon termed chromosome instability (CIN). CIN is associated with poor prognosis many cancer types, targeting attractive strategy for anti-cancer therapeutics. The mechanisms causing its contributions to tumor initiation growth not well defined, partly because there no straightforward, quantitative assays human cells. To address this problem, we have...