A5025816952- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Preterm Birth and Chorioamnionitis
- Prenatal Screening and Diagnostics
- Genomics and Phylogenetic Studies
- Enzyme Structure and Function
- Pregnancy and preeclampsia studies
- Reproductive tract infections research
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- Reproductive System and Pregnancy
- RNA Research and Splicing
Oregon Health & Science University
2007-2023
University of Washington
2007
State University of New York
1996-1999
Stony Brook University
1999
Massachusetts Institute of Technology
1996
Cervical-vaginal fluid (CVF) is a potential rich source of biomarkers for enhancing our understanding human parturition and pathologic conditions affecting pregnancy. In this study, we performed comprehensive survey the CVF proteome in pregnancy utilizing multidimensional liquid chromatography (2D-LC) coupled with mass spectrometry gel-electrophoresis-based protein separation identification. total, 150 unique proteins were identified using multiple identification algorithms. Metabolism (32%)...
Circular extrachromosomal DNA (ecDNA) in patient tumors is an important driver of oncogenic gene expression, evolution drug resistance and poor outcomes. Applying computational methods for the detection reconstruction ecDNA across a retrospective cohort 481 medulloblastoma from 465 patients, we identify circular 82 patients (18%). Patients with ecDNA-positive were more than twice as likely to relapse three times die within 5 years diagnosis. A subset harbored multiple lineages, each...
Amniotic fluid (AF) is a significant contributor to fetal health and constitutes potential rich source of biomarkers for diagnosis maternal disorders. In this study, we performed comprehensive survey the proteins expressed in AF, combining gel liquid-based fractionation approaches coupled with LC−MS/MS analysis. Two-dimensional Liquid Chromatography (2D-LC) analysis identified 118 nonredundant high confidence. One- two-dimensional electrophoresis in-gel digestion 101 proteins. Combining both...
Dominant negative inhibition is most commonly seen when a mutant subunit of multisubunit protein coexpressed with the wild-type so that assembly functional oligomer impaired. By analogy, it should be possible to interfere monomeric enzyme by interfering folding pathway. Experiments in vitro others suggested fragments might exploited for this purpose. We report here dominant bacterial cell growth expression tRNA synthetase. Inhibition fragment-specific, as not all cause inhibition. An...