A5030427730- Microbial infections and disease research
- Viral Infections and Vectors
- Animal Virus Infections Studies
- Herpesvirus Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Vector-borne infectious diseases
- Virus-based gene therapy research
- Prion Diseases and Protein Misfolding
- Intestinal and Peritoneal Adhesions
- Plant and Fungal Interactions Research
- Veterinary Oncology Research
- Sarcoma Diagnosis and Treatment
- Viral Infections and Outbreaks Research
- Toxoplasma gondii Research Studies
- Gastrointestinal Tumor Research and Treatment
- Genomics and Phylogenetic Studies
- Neurological diseases and metabolism
- Bacteriophages and microbial interactions
- Testicular diseases and treatments
- Polyamine Metabolism and Applications
- Pleural and Pulmonary Diseases
- Insect symbiosis and bacterial influences
- Surgical Sutures and Adhesives
- Neurofibromatosis and Schwannoma Cases
- Rabies epidemiology and control
Colorado State University
2013-2022
University of Montana
2012-2013
Beltsville Agricultural Research Center
2013
Agricultural Research Service
2013
California Animal Hospital
2013
ABSTRACT Chronic wasting disease (CWD), a transmissible spongiform encephalopathy of deer, elk, and moose, is the only prion affecting free-ranging animals. Since was first identified in northern Colorado southern Wyoming 1967, new epidemic foci have been 20 additional states, as well two Canadian provinces Republic South Korea. Identification CWD-affected animals currently requires postmortem analysis brain or lymphoid tissues using immunohistochemistry (IHC) an enzyme-linked immunosorbent...
Chronic wasting disease (CWD), a transmissible spongiform encephalopathy of cervids, was first documented nearly 50 years ago in Colorado and Wyoming has since been detected across North America the Republic Korea. The expansion this makes development sensitive diagnostic assays antemortem sampling techniques crucial for mitigation its spread; is especially true cases relocation/reintroduction or prevalence studies large protected herds, where depopulation may be contraindicated. This study...
Inclusion body disease (IBD) is an infectious originally described in captive snakes. It has traditionally been diagnosed by the presence of large eosinophilic cytoplasmic inclusions and associated with neurological, gastrointestinal, lymphoproliferative disorders. Previously, we identified established a culture system for novel lineage arenaviruses isolated from boa constrictors IBD. Although ample circumstantial evidence suggested that these viruses, now known as reptarenaviruses, cause...
Circumstantial evidence has linked a new group of nidoviruses with respiratory disease in pythons, lizards, and cattle. We conducted experimental infections ball pythons (Python regius) to test the hypothesis that python nidovirus (BPNV) infection results disease. Three were inoculated orally intratracheally cell culture isolated BPNV two sham inoculated. Antemortem choanal, oroesophageal, cloacal swabs postmortem tissues infected snakes positive for viral RNA, protein, infectious virus by...
Background Meningoencephalomyelitis of unknown origin ( MUO ) is a common and life‐threatening neuroinflammatory disease in dogs. Features the are suggestive an underlying immune‐mediated process, but association this with pathogen still unknown. Hypothesis/Objectives To search for candidate etiologic agent associated cases if using next generation metagenomic sequencing. Animals Twenty‐two dogs diagnosed either (11/22; 10 CSF 3 brain), or noninflammatory CNS diseases inconsistent 11 2...
The aim of this study serpentovirus infection in captive snakes was to assess the susceptibility different types and disease, survey viral genetic diversity, evaluate management practices that may limit disease. Antemortem oral swabs were collected from 639 12 US collections, including 62 species, 28 genera, 6 families: Pythonidae (N = 414 snakes; pythons overrepresented sample population), Boidae (79), Colubridae (116), Lamprophiidae (4), Elapidae (12), Viperidae (14). Infection more common...
Borrelia burgdorferi, the causative agent of Lyme disease, cycles in nature between a vertebrate host and tick vector. We demonstrate that B. burgdorferi can utilize several sugars may be available during persistence tick, including trehalose, N-acetylglucosamine (GlcNAc), chitobiose. The spirochete grows to higher cell density which is found hemolymph, than maltose; these two disaccharides differ only glycosidic linkage glucose monomers. Additionally, GlcNAc dimer chitobiose, both molting....
RNA viruses are a major source of emerging and re-emerging infectious diseases around the world. We developed method to identify that is based on fact produce double-stranded (dsRNA) while replicating. Purifying sequencing dsRNA from total isolated infected tissue allowed us recover virus sequences replicated single-stranded (ssRNA) viruses. refer this approach as dsRNA-Seq. By assembling into contigs we identified full length or partial viral genomes varying genome types infecting mammalian...
Serpentoviruses are an emerging group of nidoviruses known to cause respiratory disease in snakes and have been associated with other non-avian reptile species (lizards turtles). This study describes multiple episodes disease-associated mortalities a collection juvenile veiled chameleons (Chamaeleo calyptratus). Histopathologic lesions included rhinitis interstitial pneumonia epithelial proliferation abundant mucus. Metagenomic sequencing detected coinfection two novel serpentoviruses...
Borrelia burgdorferi, the spirochete that causes Lyme disease, differentially regulates synthesis of outer membrane lipoprotein OspC to infect its host. is required establish infection but then repressed in mammal avoid clearance by adaptive immune response. Inverted repeats (IR) upstream promoter have been implicated as an operator regulate ospC expression. We molecularly dissected distal inverted repeat (dIR) site-directed mutagenesis at endogenous location on circular plasmid cp26. found...
Burmese python (Python bivittatus) is an invasive snake that has significantly affected ecosystems in southern Florida, United States. Aside from direct predation and competition, species can also introduce nonnative pathogens adversely affect native species. The subfamily Serpentovirinae (order Nidovirales) composed of positive-sense RNA viruses primarily found reptiles. Some serpentoviruses, such as shingleback nidovirus, are associated with mortalities wild populations, while others,...
This report describes clinical, necropsy, and ancillary diagnostic findings for a mortality event in Rocky Mountain elk ( Cervus elaphus nelsoni) calves attributed to malnutrition, pasteurellosis, an alimentary presentation of adenovirus hemorrhagic disease.
A 4-year-old, female, spayed Border Collie dog was presented for progressive lethargy, inappetence, and weakness of 4 days duration. The animal had been diagnosed with pemphigus foliaceus 3 months prior receiving combination immunosuppressive therapy. Serum biochemistry revealed severely elevated liver enzymes bilirubin, humane euthanasia elected. Gross postmortem examination a diffusely pale tan to slightly yellow, enlarged, markedly friable an enhanced reticular pattern. Histologically,...
ABSTRACT This case report documents a novel late surgical complication in 2 yr old dog following an enterotomy to remove jejunal foreign body. Twenty-six days the original surgery, was re-presented with signs consistent intestinal obstruction. A mural abscess found as cause of obstruction during exploratory and site successfully removed resection anastomosis. Histopathology showed multifocal abscessation cyst-like structures partially lined mucosa. The recovered without remains healthy 4 mo...
Abstract RNA viruses are a major source of emerging and re-emerging infectious diseases around the world. We developed method to identify that is based on fact all produce dsRNA while replicating. Purifying sequencing from total isolated infected tissue allowed us recover replicating viral sequences. refer this approach as dsRNA-Seq. By assembling sequences into contigs we identified full length varying genome types infecting mammalian culture samples, known disease agent in laboratory mice,...