A5089894710- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Reproductive System and Pregnancy
- Blood groups and transfusion
- Cytomegalovirus and herpesvirus research
- Monoclonal and Polyclonal Antibodies Research
- IL-33, ST2, and ILC Pathways
- Immunodeficiency and Autoimmune Disorders
- Autoimmune Bullous Skin Diseases
- Mycobacterium research and diagnosis
- Urticaria and Related Conditions
- Glycosylation and Glycoproteins Research
- Molecular Biology Techniques and Applications
- Cancer-related molecular mechanisms research
- Systemic Lupus Erythematosus Research
- Hematopoietic Stem Cell Transplantation
- Cell Adhesion Molecules Research
- Forensic and Genetic Research
- Eosinophilic Disorders and Syndromes
- Platelet Disorders and Treatments
- Erythrocyte Function and Pathophysiology
- Atherosclerosis and Cardiovascular Diseases
- Transgenic Plants and Applications
Shenzhen Blood Center
2015-2025
Nanjing Forestry University
2016
To confirm the sequence of a null allele HLA-C*08:127N produced by base insertion. PCR sequence-specific oligonucleotide probe (SSOP) and sequence-based typing (SBT) were used for HLA routine detection, which discovered abnormal maps HLA-C in one acute myeloid leukemia patient. The above loci was confirmed next generation sequencing (NGS) technology. SSOP result showed that locus C*03:04, C*08:01, while suspected to be inserted or deleted exon 3 SBT, finally NGS as C*08:127N. When insertion...
Abstract Human natural killer (NK) cells are essential for controlling infection, cancer, and fetal development. NK cell functions modulated by interactions between polymorphic inhibitory immunoglobulin-like receptors (KIR) HLA-A, -B, -C ligands expressed on tissue cells. All HLA-C alleles encode a KIR ligand contribute to reproduction immunity. In contrast, only some HLA-A -B they focus By high-resolution analysis of genes, we show that the Chinese Southern Han (CHS) significantly enriched...
Compared with HLA‐DRB1*09:01:02:05 , the alleles HLA‐DRB1*09:57 and HLA‐DRB1*09:58 each show one nucleotide change, respectively.
The interaction of natural killer (NK) cell inhibitory receptors with polymorphic HLA class I molecules educates NK cells for immune surveillance against tumor cells. KIR A haplotype, which encodes distinct that bind certain HLA-A, -B and -C allotypes, is unusually frequent in East Asians. Focusing on Chinese southern Han, we analyzed 472 acute lymphoblastic leukemia (ALL), 433 myeloid (AML), 193 chronic (CML) patients, 745 healthy controls. We observed a higher frequency homozygosity the...
The etiology of Posner-Schlossman syndrome (PSS) remains unknown. association human leukocyte antigens (HLA) allelic diversity with PSS has been poorly investigated. To evaluate the polymorphisms class I HLA-A, -B and -C II HLA-DRB1 -DQB1 PSS, 100 unrelated patients 128 age- ethnically matched control subjects were recruited from a southern Chinese Han population. Polymorphisms in exons 2-4 for -B, loci, exon 2 2,3 HLA-DQB1 analyzed at allele haplotype levels. frequency HLA-C*1402 was...
Human leukocyte antigen (HLA)-E is a nonclassical HLA molecule with limited polymorphisms. Genotype frequency and expression of HLA-E were examined here for the first time in acute leukemia patients healthy controls. The HLA-E*01:03/*01:03 individuals was significantly higher (p = .008, OR 1.845), while HLA-E*01:01/*01:01 much lower patient group .002, .363) than control group. surface on found to be both groups, but no significant difference observed between corresponding genotypes two...
The polymorphisms of classical HLA-Ia and HLA-II loci have been associated with Posner-Schlossman syndrome (PSS) in the southern Chinese population. However, associations non-classical HLA-Ib (e.g., HLA-E HLA-G) PSS not reported for This study aimed to evaluate HLA-G a Han population group.Ninety-seven unrelated patients 90 ethnically matched control subjects were recruited from Shenzhen Eye Hospital China. full-length sequences genes amplified by long-range high-fidelity PCR, third exon...
The novel HLA‐C*07:01:19 allele differs from the closest C*07:01:01 by a single nucleotide change at coding sequence 738 G>A (codon 222 GAG>GAA) in exon 4.
Summary Previous studies showed that Burkholderia pyrrocinia JK ‐ SH 007 is a potential biocontrol agent of poplar canker disease. In this report, B. was genetically modified by introducing the Bacillus subtilis Chi113 gene to enhance its antifungal activity. The green fluorescence 007E1 can be detected because transformation with plasmid pHKT 2‐ containing gfp reporter gene. Real‐time quantitative PCR ( qPCR ) highly expressed in at mRNA level. chitinase activity and were significantly...
The novel HLA‐B *56:04:05 allele differs from its most closely related B*56:04:01:01 in exon 4.
HLA-B*13:179 differs from HLA-B*13:99 by one nucleotide substitution at position 829(A>G) in exon 4.
The novel HLA‐A*11:155 allele differs from the closest A*11:01:01:01 in exon 3.
The novel HLA-DPA1*01:11 allele differs from the closest DPA1*01:03:01 by single nucleotide change at coding sequence 242 A>G (codon 50 CAA>CGA) in exon 2.
The novel HLA‐DPA1*02:02:05 allele differs from the closest DPA1*02:02:01 by single‐nucleotide change.
The novel HLA‐B*13:82 allele differs from the closest B*13:02:01:01 in exon 3.
To study the genetic polymorphisms of human leukocyte antigen (HLA)- A, B, C, DRB1, DQA1, DQB1, DPA1and DPB1among ethnic Hans from southern China.481 randomly selected individuals were genotyped using a polymerase chain reaction (PCR) sequence-based typing (SBT) method for above genes. Their allele frequencies determined by direct counting.In total, 28 HLA-A, 57 HLA-B, HLA-C, 40 HLA-DRB1, 18 HLA-DQA1, 17 HLA-DQB1, 6 HLA-DPA1and 21 HLA-DPB1alleles identified. Among these, common alleles (with...
The novel HLA ‐ DPA1 *02:05 variant allele differs from the closest *02:02:02 .
The novel HLA-C*08:125 allele differs from the closest C*08:02:01:01 in exon 3.
Abstract HLA ‐ C*04:162 differs from the closely matching allele C*04:01:01:01 by one nucleotide substitution in exon 4 at position 883 A/G.
HLA ‐C*04:03:02 differs from the closest allele C*04:03:01 at 218T >C in exon 2 and is synonymous.
In the present study, a high-resolute method for cloning and sequencing genomic full-length HLA-A -B using 20 Chinese Han individuals was established. We detected 10 allele sequences 4.2 kb in length 6 HLA-B 3.7 length, included all exons, introns, 5'promoter, 3'UTR of two genes. All sixteen have been submitted to GenBank IMGT/HLA database. A*1153 is novel allele, introns B*151101 are firstly reported here. The 5'promoter 5 alleles 2 also disclosed, other extended full released polymorphic...
HLA ‐A*24:353 differs from ‐A*24:02:01 by an amino acid exchange glutamine to glutamate at position 316.