A5078778301- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Mosquito-borne diseases and control
- Reproductive System and Pregnancy
- Malaria Research and Control
- Zoonotic diseases and public health
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Genetic Associations and Epidemiology
- IL-33, ST2, and ILC Pathways
- Genetics and Physical Performance
- Marine Invertebrate Physiology and Ecology
- Immune responses and vaccinations
- High Altitude and Hypoxia
- Vibrio bacteria research studies
- Gut microbiota and health
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Marine Ecology and Invasive Species
- Cancer Genomics and Diagnostics
- Dengue and Mosquito Control Research
- Vector-borne infectious diseases
- Microbial Community Ecology and Physiology
- Cancer-related molecular mechanisms research
- Parasite Biology and Host Interactions
University of Colorado Denver
2024
Tempus Labs (United States)
2024
University of Colorado Anschutz Medical Campus
2019-2022
University of Colorado Boulder
2021
McGill University
2015-2019
Centre Hospitalier Universitaire Sainte-Justine
2018
Université de Montréal
2018
Walter Reed Army Institute of Research
2011-2017
Smithsonian Institution
2013
Smithsonian Marine Station
2009
Since the onset of SARS-CoV-2 pandemic, most clinical testing has focused on RT-PCR1. Host epigenome manipulation post coronavirus infection2-4 suggests that DNA methylation signatures may differentiate patients with infection from uninfected individuals, and help predict COVID-19 disease severity, even at initial presentation.We customized Illumina's Infinium MethylationEPIC array to enhance immune response detection profiled peripheral blood samples 164 longitudinal measurements severity...
Interactions of killer cell immunoglobin-like receptors (KIR) with human leukocyte antigens (HLA) class I regulate effector functions key cytotoxic cells innate and adaptive immunity. The extreme diversity this interaction is genetically determined, having evolved in the ever-changing environment pathogen exposure. Diversity KIR HLA genes further facilitated by their independent segregation on separate chromosomes. That fetal implantation relies many same types immune as infection control...
Pathogens and associated outbreaks of infectious disease exert selective pressure on human populations, any changes in allele frequencies that result may be especially evident for genes involved immunity. In this regard, the 1346-1353 Yersinia pestis-caused Black Death pandemic, with continued plague spanning several hundred years, is one most devastating recorded history. To investigate potential impact Y. pestis immunity genes, we extracted DNA from 36 victims buried a mass grave...
ABSTRACT The cyanobacterial genus Lyngbya includes free-living, benthic, filamentous cyanobacteria that form periodic nuisance blooms in lagoons, reefs, and estuaries. spp. are prolific producers of biologically active compounds deter grazers help persist the marine environment. Here, our investigations reveal presence three distinct species on nearshore reefs Broward County, FL, sampled 2006 2007. With a combination morphological measurements, molecular biology techniques, natural products...
Different human populations facing similar environmental challenges have sometimes evolved convergent biological adaptations, for example, hypoxia resistance at high altitudes and depigmented skin in northern latitudes on separate continents. The "pygmy" phenotype (small adult body size), characteristic of hunter-gatherer inhabiting both African Asian tropical rainforests, is often highlighted as another case adaptation humans. However, the degree to which phenotypic convergence this...
Abstract Human natural killer (NK) cells are essential for controlling infection, cancer, and fetal development. NK cell functions modulated by interactions between polymorphic inhibitory immunoglobulin-like receptors (KIR) HLA-A, -B, -C ligands expressed on tissue cells. All HLA-C alleles encode a KIR ligand contribute to reproduction immunity. In contrast, only some HLA-A -B they focus By high-resolution analysis of genes, we show that the Chinese Southern Han (CHS) significantly enriched...
Highly sensitive polymerase chain reaction (PCR) methods offer an alternative to the light microscopy examination of mosquito salivary glands for determination malaria sporozoite rates in wild caught female Anopheles. Removal abdomens is assumed eliminate false positives caused by oocyst DNA midgut. This assumption has not been tested with current gold standard PCR assays, and variety conditions that specimens could encounter laboratory field. Laboratory Anopheles stephensi were used had...
Genetic variation in host immunity impacts the disproportionate burden of infectious diseases that can be experienced by First Nations peoples. Polymorphic human leukocyte antigen (HLA) class I and killer cell immunoglobulin-like receptors (KIRs) are key regulators natural (NK) cells, which mediate early infection control. How this their responses across populations is unclear. We show HLA-A
Importance The National Comprehensive Cancer Network (NCCN) guidelines for non–small cell lung cancer suggest that RNA next-generation sequencing (NGS) may improve the detection of fusions and splicing variants compared with DNA-NGS alone. However, there is limited adoption RNA-NGS in routine oncology clinical care today. Objective To analyze evidence from a diverse cohort patients advanced adenocarcinoma compare NCCN-recommended actionable structural (aSVs; variants) via concurrent DNA vs...
Summary Killer cell immunoglobulin‐like receptors ( KIRs ) interact with polymorphic human leucocyte antigen HLA class I molecules, modulating natural killer (NK) functions and affecting both the susceptibility outcome of immune‐mediated diseases. The KIR locus is highly diverse in gene content, copy number allelic polymorphism within individuals across geographical populations. To analyse currently under‐represented Asian Pacific populations, we investigated combinatorial diversity 92...
Highly polymorphic interaction of KIR3DL1 and KIR3DS1 with HLA class I ligands modulates the effector functions natural killer (NK) cells some T cells. This genetically determined diversity affects severity infections, immune-mediated diseases, cancers, impacts course immunotherapies, including transplantation. is an inhibitory receptor, activating receptor encoded by KIR3DL1/S1 gene that has more than 200 diverse divergent alleles. Determination genotypes for medical application hampered...
Vivax malaria is a significant military and civilian health threat in the north of Republic Korea (ROK). The island Baengnyeong-do westernmost point ROK located close to southwestern coast Democratic People's (DPRK). Mosquitoes were collected using black light trap on Baengnyeong-do, Anopheles spp. assayed by PCR, identify species, screened for sporozoites Plasmodium vivax. Of subsample 257 mosquitoes, lesteri was most frequently (49.8%), followed sinensis (22.6%), pullus (18.7%), kleini...
Despite first being described from Virginia, the widely distributed brackish water hydrozoan Blackfordia virginica is often hypothesized to have been introduced Black Sea United States. However, alternative view that B. was also persists in literature. This study investigates population structure of States assess directionality and/or number introduction events. During 2009 and 2010, estuaries were sampled Delaware Louisiana for hydromedusae. Nineteen samples collected four localities,...
Malaria continues to be a major health threat near the Demilitarized Zone (DMZ) that separates North and South Korea. Adult mosquitoes were collected from 20 July through 21 October, 2010 at Daeseongdong, small village within DMZ. Molecular techniques used identify Anopheles species for detection of Plasmodium vivax sporozoites in their head thorax. Trap catches showed concordant peaks belenrae An. kleini early study period pullus sinensis later season. Three well defined 107 sporozoite...
The Malaria Research and Reference Reagent Resource–recommended PLF/UNR/VIR polymerase chain reaction (PCR) was used to detect Plasmodium vivax in Anopheles spp. mosquitoes collected South Korea. Samples that were amplified sequenced compared with known by using the PlasmoDB.org Basic Local Alignment Search Tool/n National Center for Biotechnology Information tools. Results show primers this PCR can produce uninterpretable results non-specific sequences field-collected mosquitoes. Three...
Arthropod-borne diseases remain a pressing international public health concern. While progress has been made in the rapid detection of arthropod-borne pathogens via quantitative real-time (qPCR), or even hand-held devices, simple and robust maceration nucleic acid extraction method is necessary to implement biosurveillance capabilities. In this study, comparison techniques using five types beads followed by were tested two morphologically disparate arthropods, Aedes aegypti mosquito...
Weekly changes in adult Anopheles species were monitored at Camp Humphreys (CH), Ganghwa Island (GH), and Warrior Base (WB), from May-October, 2009-2010 to explore the relationship between Plasmodium vivax development vector dynamics Republic of Korea (ROK). Adult females trapped dissected determine parity for estimating longevity, mortality, birthrate. A degree-day (DD) method was used estimate extrinsic incubation period (EIP) P. duration gonotrophic cycle other life stages. sinensis...
Abstract Human natural killer (NK) cells are essential for controlling infection, cancer and fetal development. NK cell functions modulated by interactions between polymorphic inhibitory immunoglobulin-like receptors (KIR) HLA-A, -B -C ligands expressed on tissue cells. All HLA-C alleles encode a KIR ligand contribute to reproduction immunity. In contrast, only some HLA-A they focus By high-resolution analysis of , genes, we show that the Chinese Southern Han significantly enriched -B. This...
Abstract The shift from a hunter-gatherer (HG) to an agricultural (AG) mode of subsistence is believed have been associated with profound changes in the burden and diversity pathogens across human populations. Yet, extent which advent agriculture may impacted evolution immune system remains unknown. Here we present comparative study variation transcriptional responses peripheral blood mononuclear cells bacterial viral stimuli between Batwa rainforest hunter-gatherers Bakiga agriculturalists...
Abstract Since the onset of SARS-CoV-2 pandemic, most clinical testing has focused on RT-PCR. Host epigenome manipulation post coronavirus infection suggests that DNA methylation signatures may differentiate patients with from uninfected individuals, and help predict COVID-19 disease severity, even at initial presentation. We customized Illumina’s Infinium Methylation EPIC array to enhance immune response detection profiled peripheral blood samples 164 longitudinal measurements severity 296...
Abstract Highly polymorphic interactions of KIR3DL1 and KIR3DS1 with HLA class I ligands modulates the effector functions natural killer (NK) cells some T cells. This genetically determined diversity affects severity infections, immune-mediated diseases, cancers, impacts course cancer treatment, including transplantation. is an inhibitory receptor, activating receptor encoded by KIR3DL1/S1 gene that has more than 200 diverse divergent alleles. Determination genotypes for medical application...
Abstract Different human populations facing similar environmental challenges have sometimes evolved convergent biological adaptations, for example hypoxia resistance at high altitudes and depigmented skin in northern latitudes on separate continents. The pygmy phenotype (small adult body size), a characteristic of hunter-gatherer inhabiting both African Asian tropical rainforests, is often highlighted as another case adaptation humans. However, the degree to which phenotypic convergence this...