A5005423495- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Renal Transplantation Outcomes and Treatments
- Hematopoietic Stem Cell Transplantation
- Blood groups and transfusion
- Immunotherapy and Immune Responses
- Cytomegalovirus and herpesvirus research
- Reproductive System and Pregnancy
- Immunodeficiency and Autoimmune Disorders
- Transplantation: Methods and Outcomes
- Olfactory and Sensory Function Studies
- Organ Donation and Transplantation
- Psoriasis: Treatment and Pathogenesis
- Spondyloarthritis Studies and Treatments
- vaccines and immunoinformatics approaches
- SARS-CoV-2 and COVID-19 Research
- Evolutionary Psychology and Human Behavior
- COVID-19 Clinical Research Studies
- Glycosylation and Glycoproteins Research
- Gene expression and cancer classification
- Palliative Care and End-of-Life Issues
- Diabetes and associated disorders
- Hemoglobinopathies and Related Disorders
- Immune responses and vaccinations
- Genetic Associations and Epidemiology
Deutsche Knochenmarkspenderdatei
2016-2025
Memorial Sloan Kettering Cancer Center
2025
German National Bone Marrow Donor Registry
2014-2025
Areté Associates (United States)
2023
Abstract Background A close match of the HLA alleles between donor and recipient is an important prerequisite for successful unrelated hematopoietic stem cell transplantation. To increase chances finding donor, registries recruit many hundred thousands volunteers each year. Many with limited resources have had to find a trade-off cost resolution extent typing newly recruited donors in past. Therefore, we taken advantage recent improvements NGS develop workflow low-cost, high-resolution...
A catalog of common, intermediate and well‐documented (CIWD) HLA‐A, ‐B, ‐C, ‐DRB1, ‐DRB3, ‐DRB4, ‐DRB5, ‐DQB1 ‐DPB1 alleles has been compiled from over 8 million individuals using data 20 unrelated hematopoietic stem cell volunteer donor registries. Individuals are divided into seven geographic/ancestral/ethnic groups summarized for each group the total population. P (two‐field) G assignments one four frequency categories: common (≥1 in 10 000), 100 (≥5 occurrences) or not‐CIWD. Overall 26%...
At the DKMS Life Science Lab, Next Generation Sequencing (NGS) has been used for ultra-high-volume high-resolution genotyping of HLA loci last three and a half years. Here, we report on our experiences in HLA, CCR5, ABO, RHD KIR genes using direct amplicon sequencing approach Illumina MiSeq HiSeq 2500 instruments.Between January 2013 June 2016, 2,714,110 samples largely from German, Polish UK-based potential stem cell donors have processed. 98.9% all alleles targeted (HLA-A, -B, -C, -DRB1,...
Homozygous carriers of CCR5-Δ32, a gene variant CC-type chemokine receptor 5 (CCR5), are highly resistant to infections with human immunodeficiency virus type 1 (HIV-1) and therefore preferred stem cell donors for HIV-infected patients. We analyzed CCR5 typing data 1,333,035 potential hematopoietic enlisted three national DKMS donor centers. Allele genotype frequencies were determined 87 countries origin as self-assessed by the donors. CCR5-Δ32 allele ranged from 16.4% in Norwegian sample 0...
Optimizing natural killer (NK) cell alloreactivity could further improve outcome after allogeneic hematopoietic transplantation (alloHCT). The donor’s Killer-cell Immunoglobulin-like Receptor (KIR) genotype may provide important information in this regard. In the past decade, different models have been proposed aiming at maximizing NK activation by activating KIR-ligand interactions or minimizing inhibitory interactions. Alternative classifications intended predicting alloHCT donor...
Background A catalogue of common and well‐documented (CWD) human leukocyte antigen (HLA), previously established by the American Society for Histocompatibility Immunogenetics (ASHI), is widely used as indicator typing ambiguities to be resolved in tissue transplantation or checking universality any HLA allele world. However, European population samples, which are characterized a substantial level genetic variation, underrepresented ASHI catalogue. Therefore, Population Genetics Working Group...
Leprosy, a chronic infectious disease caused by Mycobacterium leprae (M. leprae), was very common in Europe till the 16th century. Here, we perform an ancient DNA study on medieval skeletons from Denmark that show lesions specific for lepromatous leprosy (LL). First, test remains M. to confirm infection status of individuals and assess bacterial diversity. We assemble 10 complete genomes all differ each other. Second, evaluate whether human leukocyte antigen allele DRB1*15:01, strong LL...
The killer-cell immunoglobulin-like receptor (KIR) genes regulate natural killer cell activity, influencing predisposition to immune mediated disease, and affecting hematopoietic stem transplantation outcome. Owing the complexity of KIR locus, with extensive gene copy number variation allelic diversity, high-resolution characterization has so far been applied only relatively small cohorts. Here, we present a comprehensive high-throughput genotyping approach based on next generation...
The characterization of the ABO blood group status is vital for transfusion and solid organ transplantation. Several methods molecular gene, which encodes alleles that give rise to different groups, have been described. However, application those has so far restricted selected samples not applied population-scale analysis. We describe a cost-effective method high-throughput genotyping system by next generation sequencing. Sample specific barcodes sequencing adaptors are introduced during...
Mothers are able to identify the body odour (BO) of their own child and prefer this smell above other BOs. It has hence been assumed that infantile BO functions as a chemosignal promoting targeted parental care. We tested hypothesis examined whether children's BOs signal genetic similarity developmental status mothers. In addition, we assessed facilitate inbreeding avoidance (Westermarck effect). cross-sectional design, N = 164 mothers participated with biological children ( 226 children,...
Pathogens and associated outbreaks of infectious disease exert selective pressure on human populations, any changes in allele frequencies that result may be especially evident for genes involved immunity. In this regard, the 1346-1353 Yersinia pestis-caused Black Death pandemic, with continued plague spanning several hundred years, is one most devastating recorded history. To investigate potential impact Y. pestis immunity genes, we extracted DNA from 36 victims buried a mass grave...
Allogeneic hematopoietic stem cell (HSC) transplantation is a curative therapy for many severe blood diseases. As patients have no suitable family donor, large unrelated donor registries and centers been established in countries, along with an international system the provision of HSC products. essential part this system, DKMS operates 7 countries total 12.2 million donors over 114,000 donations so far, multinational registry. In 2022, contributed 57.5% all cross-border worldwide. review, we...
Knowledge of HLA haplotypes is helpful in many settings as disease association studies, population genetics, or hematopoietic stem cell transplantation. Regarding the recruitment unrelated donors, haplotype frequencies specific populations are used to optimize both donor searches for individual patients and strategic registry planning. However, estimation from genotyping data challenged by large amount genotype data, complex nomenclature, heterogeneous ambiguous nature typing records.To meet...
Abstract The major histocompatibility complex (MHC, called HLA in humans) is an important genetic component of the immune system. Fish, birds and mammals prefer mates with different MHC code compared to their own, which they determine using olfactory cues. This preference increases chances high variety offspring, leading enhanced resilience against a pathogens. Humans are also able discriminate related stimuli, however, it debated whether this mechanism behavioural relevance. We show on...
NK cells expressing self-inhibitory receptors display increased functionality compared to lacking those receptors. The acquisition of functional competence in these particular NK-cell subsets is termed education. Little known about the underlying mechanisms that lead differences between educated and uneducated cells. An increasing number studies suggest cellular metabolism a determinant immune cell functions. Thus, alterations metabolic pathways may play role process Here, we glycolytic...
MICA and MICB are ligands of the NKG2D receptor thereby influence NK T cell activity. MICA/B gene polymorphisms, expression levels amount soluble in serum have been linked to autoimmune diseases, infections, cancer. In hematopoietic stem transplantation, matching between donor patient has correlated with reduced acute chronic graft-vs.-host disease improved survival. Hence, we developed an extremely cost-efficient high-throughput workflow for genotyping newly registered potential donors....
The killer-cell immunoglobulin-like receptor ( KIR) complex on chromosome 19 encodes receptors that modulate the activity of natural killer cells, and variation in these genes has been linked to infectious autoimmune disease, as well having bearing pregnancy transplant outcomes. medical relevance high variability KIR makes short-read sequencing an attractive technology for interrogating region, providing a high-throughput, high-fidelity method is cost-effective. However, because this gene...
Missing data problems are common in biological, high-dimensional data, where can be partially or completely missing. Algorithms have been developed to reconstruct the missing values by means of imputation expectation-maximization algorithms. For problems, it has suggested that regression model interest should incorporated into procedure reduce bias coefficients. We here consider a challenging problem, diplotypes KIR loci reconstructed. These difficult genotype, resulting ambiguous genotype...
In hematopoietic stem cell transplantation, optimal results are achieved when donors and patients matched regarding their human leukocyte antigen (HLA) genes. Population-specific HLA allele haplotype frequency distributions determine the probabilities to find in a donor registry of given size ethnic composition. To evaluate needs Indian with regard future recruitment, we analyzed large data set n=130,518 potential registered DKMS-BMST, Bangalore-based nationwide recruitment activities. We...