A5028168426- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- RNA and protein synthesis mechanisms
- CAR-T cell therapy research
- RNA Research and Splicing
- Viral Infections and Immunology Research
- Cell Image Analysis Techniques
- COVID-19 Clinical Research Studies
- Bacteriophages and microbial interactions
- Advanced Fluorescence Microscopy Techniques
- CRISPR and Genetic Engineering
- Optical Coherence Tomography Applications
- Urological Disorders and Treatments
- Image Processing Techniques and Applications
- interferon and immune responses
- Historical Art and Culture Studies
- Cytomegalovirus and herpesvirus research
- Historical Astronomy and Related Studies
- Renaissance Literature and Culture
- Ubiquitin and proteasome pathways
- Neurofibromatosis and Schwannoma Cases
Universidad Autónoma del Estado de Morelos
2014-2024
Leibniz Institute of Virology (LIV)
2021-2024
Life Length (Spain)
2024
Universidad Nacional Autónoma de México
2015-2016
Hospital Clínico San Carlos
2012
Adenoviruses are a group of double-stranded DNA viruses that can mainly cause respiratory, gastrointestinal, and eye infections in humans. In addition, adenoviruses employed as vector vaccines for combatting viral infections, including SARS-CoV-2, serve excellent gene therapy vectors. These have the ability to modulate host cell machinery their advantage trigger significant restructuring nuclei infected cells through activity proteins. One those, adenovirus DNA-binding protein (DBP), is...
Adenovirus (Ad) replication compartments (RC) are nuclear microenvironments where the viral genome is replicated and a coordinated program of late gene expression established. These virus-induced sites seem to behave as central hubs for regulation virus-host cell interactions, since proteins that promote efficient well factors participate in antiviral response coopted concentrated there. To gain further insight into activities RC, here we report, first time, morphology, composition, RC...
A common viral replication strategy is characterized by the assembly of intracellular compartments that concentrate factors needed for and simultaneously conceal genome from host-defense mechanisms. Recently, various membrane-less virus-induced cellular organelles have been shown to represent biomolecular condensates (BMCs) assemble through liquid-liquid phase separation (LLPS). In present work, we analyze biophysical properties intranuclear (RCs) induced during human adenovirus (HAdV)...
The multifunctional adenoviral E1B-55K phosphoprotein is a major regulator of viral replication and plays key roles in virus-mediated cell transformation. While much known about its function oncogenic transformation, the underlying features exact mechanisms that implicate regulation gene expression are less well understood. Therefore, this work aimed to unravel basic intranuclear principles E1B-55K-regulated mRNA biogenesis using wild-type human adenovirus C5 (HAdV-C5) E1B-55K, virus mutant...
Abstract Viruses employ a variety of strategies to hijack cellular activities through the orchestrated recruitment macromolecules specific virus-induced micro-environments. Adenoviruses (Ad) and other DNA viruses induce extensive reorganization cell nucleus formation nuclear Replication Compartments (RCs), where viral genome is replicated expressed. In this work an automatic algorithm designed for detection segmentation RCs using ellipses presented. Unlike algorithms available in literature,...
The adenovirus type 5 (Ad5) E1B 55 kDa and E4 Orf6 proteins assemble a Cullin 5-E3 ubiquitin (Ub) ligase that targets, among other cellular proteins, p53 the Mre11-Rad50-Nbs1 (MRN) complex for degradation. latter is also inhibited by Orf3 protein, which promotes recruitment of Mre11 into specific nuclear sites to promote viral DNA replication. activities associated with depend mostly on assembly this E3-Ub ligase. However, can function as an E3-SUMO ligase, suggesting not only regulation...
During infection of human cells by adenovirus (Ad), the host cell nucleus is dramatically reorganized, leading to formation nuclear microenvironments through recruitment viral and cellular proteins sites occupied genome. These sites, called replication compartments (RC), can be considered viral-induced domains where genome localized that participate in replication, transcription post-transcriptional processing are recruited. Moreover, involved antiviral response, such as tumor suppressor...
The E1B 55kDa produced by human adenovirus type 5 is a multifunctional protein that participates in the regulation of several steps during viral replication cycle. Previous studies suggest this plays an important role postranscriptional and cellular gene expression, as it required for selective accumulation maximal levels late mRNA cytoplasm infected cell; however molecular mechanisms are altered or regulated have not been elucidated. A ribonucleoprotein motif could implicate direct...
The adenovirus type 5 (HAdV-C5) E1 transcription unit encodes regulatory proteins that are essential for viral replication and transformation. Among these, E1A E1B-55K act as key multifunctional HAdV-C5 involved in various steps of the cycle virus-induced cell In this context, HAdV-C5-mediated dysregulations cellular factors such tumor suppressors p53 pRB have been intensively investigated. However, components downstream events could affect infection transformation widely unknown. We...
Abstract Purpose To assess the clinical performance of ProsTAV ® , a blood-based test based on telomere associate variables (TAV) measurement, to support biopsy decision-making when diagnosing suspicious prostate cancer (PCa). Methods Preliminary data prospective observational pragmatic study patients with prostate-specific antigen (PSA) levels 3–10 ng/ml and PCa. Results were combined other data, all underwent biopsies according each center’s routine practice, while magnetic resonance...
ABSTRACT Human adenoviruses are double-stranded DNA viruses that replicate in the cell nucleus and induce formation of replication compartments (RCs) critical viral control virus-host interactions. RCs specialized virus-induced subnuclear microenvironments where not only genome expression orchestrated but also host proteins restrict co-opted subverted. The protein composition these remains largely unexplored. In this study, we isolated adenovirus RC-enriched fractions from infected cells at...
During infection of human cells by adenovirus (Ad), the host cell nucleus is dramatically reorganized, leading to formation nuclear microenvironments through recruitment viral and cellular proteins sites occupied genome. These sites, called replication compartments (RC), can be considered viral-induced domains where genome localized that participate in replication, transcription post-transcriptional processing are recruited. Moreover, involved antiviral response, such as tumor suppressor...