A5047332247- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Viral Infections and Immunology Research
- Metabolism and Genetic Disorders
- Heat shock proteins research
- Lysosomal Storage Disorders Research
- Biochemical and Molecular Research
- Cardiomyopathy and Myosin Studies
- Glycogen Storage Diseases and Myoclonus
- Mitochondrial Function and Pathology
- thermodynamics and calorimetric analyses
- Dermatological and Skeletal Disorders
- Calcium signaling and nucleotide metabolism
- Genetic Neurodegenerative Diseases
- Neurological and metabolic disorders
- Connective tissue disorders research
- Parasites and Host Interactions
- Renal Diseases and Glomerulopathies
- Genetic and Kidney Cyst Diseases
- Eosinophilic Disorders and Syndromes
- Neonatal Health and Biochemistry
- Neurological disorders and treatments
- Genomic variations and chromosomal abnormalities
- Diet and metabolism studies
- Neurogenetic and Muscular Disorders Research
University of North Carolina Health Care
2024
Northwestern University
2010-2022
Cedars-Sinai Medical Center
2017-2022
University of California, Los Angeles
2020-2021
Cedars-Sinai Smidt Heart Institute
2018
American Journal Experts (United States)
2018
Columbia University
2013
Midwestern University
2011
Ferrari (Italy)
2010
Cardiovascular Institute Hospital
2001
Rationale: Efficient clearance of apoptotic cells (efferocytosis) is a prerequisite for inflammation resolution and tissue repair. After myocardial infarction, phagocytes are recruited to the heart promote dying cardiomyocytes. The molecular mechanisms efferocytosis cardiomyocytes in myocardium unknown. injured provides unique model examine relationships between subsequent resolution, remodeling, organ function. Objective: We set out identify cardiomyocyte engulfment by and, first time,...
MD-PhD training programs provide an integrated approach for physician-scientists. The goal of this study was to characterize the career path taken by program alumni during past 40 years and identify trends that affect their success.In 2007-early 2008, 24 enrolling 43% current trainees representing half National Institutes Health-funded submitted anonymous data on 5,969 former trainees.The average enrolled 90 trainees, required 8.0 complete, had attrition rate 10%. Nearly all (95%) those who...
In Trypanosomatidae the messenger RNA's (mRNA's) that code for variant surface glycoproteins (VSG's), tubulins, calmodulin, and at least a subset of other proteins contain common 35-nucleotide leader sequence their 5' ends. Hybrid-arrested in vitro translation has been used to show all mRNA's both African South American trypanosomes this sequence. Oligonucleotides complementary blocked trypanosome rabbit reticulocyte lysate system, but did not inhibit from organisms lacking An...
The eukaryotic flagellar membrane has a distinct composition from other domains of the plasmalemma. Our work shows that specialized trypanosome reflects increased concentrations sterols and saturated fatty acids, correlating with direct observation high liquid order by laurdan fluorescence microscopy. These findings indicate possesses lipid rafts: discrete regions lateral heterogeneity in plasma membranes serve to sequester organize protein complexes. Consistent this, dually acylated Ca2+...
Leishmania species engineered to express high levels of the surface metalloprotease gp63 have enhanced capacity migration through extracellular matrix in vitro. This correlates with degradation components, such as collagen type IV and fibronectin, suggests an important role for pathogenesis leishmaniasis.
Antimicrobial peptides are components of the innate immune systems a wide variety eukaryotic organisms and being developed as antibiotics in fight against bacterial fungal infections. We explored potential activities antimicrobial African trypanosome Trypanosoma brucei, vector-borne protozoan parasite that is responsible for significant morbidity mortality both humans animals. Three classes mammalian were tested: α-defensins, β-defensins, cathelicidins. Although members all 3 showed...
The major zinc metalloprotease of<i>Leishmania</i> (gp63), an important determinant of parasite virulence, is attached to the surface via a glycosylphosphatidylinositol anchor. Here we report spontaneous release proteolytically active gp63 from number isolates, causing cutaneous and visceral disease. To investigate mechanism(s) release, transfected gp63-deficient variant <i>Leishmania amazonensis</i> with constructs expressing various mutants thereof. Surprisingly, approximately half wild...
Summary Human infection by the vector‐borne protozoan Leishmania is responsible for substantial worldwide morbidity and mortality. The surface‐metalloprotease (leishmanolysin) of a virulence factor which contributes to variety functions including evasion complement‐mediated parasite‐killing host intramacrophage survival. We tested hypothesis that leishmanolysin serves protect parasites from cytolytic effects various antimicrobial peptides (AMPs) are important components innate immune system....
Abstract A 24-kDa protein of Trypanosoma cruzi, the protozoan parasite that causes Chagas' disease, is recognized by antisera from both humans and experimental animals infected with this organism. Near its C terminus are two regions have sequence similarity several Ca2+-binding proteins conform to E-F hand structure. We expressed a cDNA encoding in Escherichia coli showed recombinant native trypanosome do indeed bind Ca2+. The protein's low capacity (less than 2 mol Ca2+/mol protein) high...
We have isolated a gene from the protozoan parasite Trypanosoma cruzi that encodes previously unidentified member of 70-kilodalton heat shock protein (hsp70) family. Among all eucaryotic hsp70 proteins described to date, this trypanosome protein, mtp70, is uniquely related in sequence and structure Escherichia coli, DnaK, which functions initiation DNA replication. This relationship DnaK especially relevant view intracellular location protein. Within trypanosome, mtp70 located mitochondrion,...
Many eukaryotic proteins are posttranslationally modified by the esterification of cysteine thiols to long-chain fatty acids. This modification, protein palmitoylation, is catalyzed a large family palmitoyl acyltransferases that share an Asp-His-His-Cys Cys-rich domain but differ in their subcellular localizations and substrate specificities. In Trypanosoma brucei, flagellated protozoan parasite causes African sleeping sickness, palmitoylation has been observed for few proteins, extent...
ABSTRACT Infection with Trypanosoma cruzi , the agent of Chagas' disease, may induce antibodies and T cells reactive self antigens (autoimmunity). Because autoimmunity is generally thought to develop during chronic phase infection, one hypothesis that develops only after long-term, low-level stimulation self-reactive cells. However, preliminary reports suggest begin acute T. infection. The goal present study was investigate whether cardiac could be observed A/J mice infected Brazil strain...
Abstract Immunologic screening of cDNA expression libraries has been widely used for the identification DNA sequences encoding immunologically relevant proteins many pathogenic microorganisms. For reasons that are not entirely clear, 70-kDa heat shock and related (hsp70), which among most highly conserved known, have routinely identified by this approach. Consequently, hsp70 proposed to be involved in autoimmune processes thought responsible pathogenesis diseases caused some these organisms,...
Protein palmitoylation has diverse effects in regulating protein membrane affinity, localization, binding partner interactions, turnover and function. Here, we show that also contributes to the sorting of proteins eukaryotic flagellum. African trypanosomes are protozoan pathogens express a family unique Ca2+-binding proteins, calflagins, which undergo N-terminal myristoylation palmitoylation. The localization calflagins depends on their acylation status. Myristoylation alone is sufficient...