A5077135460- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Studies on Chitinases and Chitosanases
- COVID-19 diagnosis using AI
- Insect Resistance and Genetics
- Parasites and Host Interactions
- Transgenic Plants and Applications
- Misinformation and Its Impacts
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- COVID-19 and Mental Health
- Viral Infectious Diseases and Gene Expression in Insects
Universidade Federal do ABC
2020-2024
The data presented here is related to negative results obtained with the recombinant expression of chitinase from four species Leishmania parasites in two systems, performed order investigate molecular characteristics and its possible application leishmaniasis diagnosis. Thus, heterologous sp proteins were expressed bacteria using prokaryotic vector pET28a Escherichia coli Mach-T1, Spodoptera frugiperda (Sf9) insect cells, eukaryotic bac-to-bac system (Thermo Fisher Scientific) produce...
Abstract Leishmaniasis, a neglected infectious disease affecting humans, domestic and wild animals, caused by 20 from 53 Leishmania genus species, is transmitted sandflies. genus, belonging to Trypanosomatide Family Kinetoplastida Order, are grouped in five subgroups according biogeographic evolutive history of parasites hosts, which has led incongruences paraphyly. The GH18 chitinase, encoded specie-specific single copy gene, conserved basal groups trypanosomatids, absent the Trypanosoma ,...
Introduction: Leishmaniasis, a neglected infectious disease affecting humans, domestic, and wild animals, is caused by 20 from 53 Leishmania genus species transmitted sandflies. genus, belonging to the Trypanosomatidae family Kinetoplastida order, are grouped into five subgroups according biogeographic evolutive history of parasites hosts, leading incongruences paraphyly. The GH18 chitinase, which encoded species-specific single-copy gene, conserved in basal groups trypanosomatids, absent...
Baculovirus expression vector systems (BEVS) have been widely used for production of recombinant proteins in insect cells. However, baculoviruses superinfection and repeated passages originate defective interfering particle (DIP) mutants, which is a limitation to continuous large-scale production. Accordingly, classical chemical transfection method performed on monolayer Spodoptera frugiperda cells (Sf9) was modified produce with high efficiency. Modifications consist transfect exponentially...