A5075147654- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Protein Degradation and Inhibitors
- Nanoplatforms for cancer theranostics
- Nanoparticle-Based Drug Delivery
- Microtubule and mitosis dynamics
- Multiple Myeloma Research and Treatments
- CAR-T cell therapy research
- Wnt/β-catenin signaling in development and cancer
- Graphene and Nanomaterials Applications
- Histone Deacetylase Inhibitors Research
- RNA Interference and Gene Delivery
University of Würzburg
2019-2025
Universitätsklinikum Würzburg
2023
Oncode Institute
2023
Helmholtz Zentrum München
2023
Ludwig-Maximilians-Universität München
2023
Goethe Institute
2023
Goethe University Frankfurt
2023
Technion – Israel Institute of Technology
2023
Weatherford College
2023
National Institute of Technology Warangal
2017
SPT6 is a histone chaperone that tightly binds RNA polymerase II (RNAPII) during transcription elongation. However, its primary role in uncertain. We used targeted protein degradation to rapidly deplete human cells and analyzed defects RNAPII behavior by multi-omics approach mathematical modeling. Our data indicate crucial factor for processivity therefore required the productive of protein-coding genes. Unexpectedly, also has vital termination, as acute depletion induced readthrough...
Abstract The contribution of deubiquitylating enzymes (DUBs) to β-Catenin stabilization in intestinal stem cells and colorectal cancer (CRC) is poorly understood. Here, by using an unbiassed screen, we discovered that the DUB USP10 stabilizes specifically APC-truncated CRC vitro vivo. Mechanistic studies, including binding together with computational modelling, revealed mediated via unstructured N-terminus outcompeted intact APC, favouring β-catenin degradation. However, binds β-catenin,...
Targeted protein degradation using degrons, such as the mini-Auxin-inducible degron (mAID), has an advantage over genetic silencing/knockout. However, efficiency of sgRNA, homologous recombination, tedious expansion, and screening single clones makes process tagging endogenous proteins long laborious. This protocol describes a practical economical way to obtain AID-tagged CRISPR/Cas9-mediated homology-directed repair (HDR). We use generation endogenously SPT6 in U2OS cells example but...
Transcripts produced by RNA polymerase II (RNAPII) are fundamental for cellular responses to environmental changes. It is therefore no surprise that there exist multiple avenues the regulation of this process. To explore mediated RNAPII-interacting proteins, we used a small interfering (siRNA)-based screen systematically evaluate their influence on synthesis. We identified several proteins strongly affected RNAPII activity. evaluated one top hits, SCAF1 (SR-related C-terminal...
<title>Abstract</title> The contribution of deubiquitylating enzymes (DUBs) to b-Catenin stabilization in intestinal stem cells and colorectal cancer (CRC) is poorly understood. Here, by using an unbiassed screen, we discovered that the DUB USP10 stabilizes specifically APC-truncated CRC <italic>in vitro</italic> vivo</italic>. Mechanistic studies, including binding together with computational modelling, revealed mediated via unstructured N-terminus outcompeted intact APC, favouring...