A5065097383- Ubiquitin and proteasome pathways
- Wnt/β-catenin signaling in development and cancer
- Cancer-related gene regulation
- Vector-borne infectious diseases
- T-cell and B-cell Immunology
- Inflammatory Bowel Disease
- Diverticular Disease and Complications
- Autoimmune and Inflammatory Disorders
- Cancer, Lipids, and Metabolism
- Cardiac tumors and thrombi
- Epigenetics and DNA Methylation
- Immunodeficiency and Autoimmune Disorders
- RNA modifications and cancer
- Renal Transplantation Outcomes and Treatments
- Viral Infections and Vectors
- Vascular Tumors and Angiosarcomas
- Sarcoma Diagnosis and Treatment
- Cancer-related Molecular Pathways
- Lipid metabolism and biosynthesis
- Hematopoietic Stem Cell Transplantation
- Microtubule and mitosis dynamics
- Microscopic Colitis
- Cancer Research and Treatments
- Histone Deacetylase Inhibitors Research
- Immune Cell Function and Interaction
Universitätsklinikum Würzburg
2017-2025
University of Würzburg
2022-2025
Comprehensive Cancer Center Mainfranken
2023-2025
SREBP2 is a master regulator of the mevalonate pathway (MVP), biosynthetic process that drives synthesis dolichol, heme A, ubiquinone and cholesterol also provides substrates for protein prenylation. Here, we identify as novel substrate USP28, deubiquitinating enzyme frequently upregulated in squamous cancers. Our results show silencing USP28 reduces expression MVP enzymes lowers metabolic flux into this pathway. We binds to mature SREBP2, leading its deubiquitination stabilisation....
Dedifferentiated liposarcoma (DDLPS) is a rare mesenchymal cancer originating from the adipose tissue, with poor survival rates for most patients, highlighting critical need novel treatment options. This report examines efficacy and safety of sequential pre-treatment marine-derived alkaloid trabectedin followed by checkpoint inhibition using anti-PD-1 antibody nivolumab in 63-year-old male patient unresectable retroperitoneal DDLPS. Treatment was initiated at time seventh relapse as part...
Abstract Postoperative disease recurrence in Crohn’s represents a relevant issue despite recent advancements surgical and medical therapies. Additional criteria are necessary to improve the identification of patients at risk enable selective therapeutic approaches. The role resection margins on remains unclear general recommendations lacking. A single-center retrospective analysis was performed including all who received ileocecal due disease. Resection were analyzed by two independent...
BACKGROUNDAntibiotic-Refractory Lyme Arthritis (ARLA) involves a complex interplay of T cell responses targeting Borrelia burgdorferi antigens progressing toward autoantigens by epitope spreading. However, the precise molecular mechanisms driving pathogenic response in ARLA remain unclear. Our aim was to elucidate program disease-specific Th cells.METHODSUsing flow cytometry, high-throughput receptor (TCR) sequencing, and scRNA-Seq CD4+ cells isolated from joints patients with living Europe,...
Abstract The two p53 homologues p63 and p73 regulate transcriptional programs in epithelial tissues several cell types these express both proteins. All members of the family form tetramers their active state through a dedicated oligomerization domain that structurally assembles as dimer dimers. share high sequence identity, but is more divergent it lacks functionally important C-terminal helix present other members. Based on structural differences, does not hetero-oligomerize with or p73. In...
Abstract The contribution of deubiquitylating enzymes (DUBs) to β-Catenin stabilization in intestinal stem cells and colorectal cancer (CRC) is poorly understood. Here, by using an unbiassed screen, we discovered that the DUB USP10 stabilizes specifically APC-truncated CRC vitro vivo. Mechanistic studies, including binding together with computational modelling, revealed mediated via unstructured N-terminus outcompeted intact APC, favouring β-catenin degradation. However, binds β-catenin,...
Abstract Molecular understanding of osteogenic differentiation (OD) human bone marrow-derived mesenchymal stem cells (hBMSCs) is important for regenerative medicine and has direct implications cancer. We report that the RNF4 ubiquitin ligase essential OD hBMSCs, RNF4-deficient hBMSCs remain as stalled progenitors. Remarkably, incubation in conditioned media differentiating restored OD. Transcriptional analysis RNF4-dependent gene signatures identified two secreted factors act downstream...
Background: Antibiotic-Refractory Lyme Arthritis (ARLA) involves a complex interplay of T cell responses targeting Borrelia burgdorferi antigens succeeding towards autoantigens by epitope spreading. However, the precise molecular mechanisms driving pathogenic response in ARLA remain unclear. Our aim was to elucidate program disease-specific Th cells. Methods: Using flow cytometry, high-throughput receptor (TCR) sequencing and scRNA-seq CD4+ cells isolated from joints European patients, we...
Abstract Background Postoperative disease recurrence after ileocecal resection for Crohn`s Disease is a major issue all disciplines. While adequate therapeutic strategies are lacking, further criteria necessary to improve the identification of patients at risk recurrence. In line with that, exact role positive margins as potential factor on remains unclear since current evidence heterogenous. Methods All who received due localized terminal ileitis Crohn Department Surgery University Hospital...
Summary The contribution of deubiquitylating enzymes to β-Catenin stabilisation in intestinal stem cells and colorectal cancer (CRC) is poorly understood. Here, we report the deubiquitylase USP10 as an APC-truncation- specific enhancer stability, potentiating WNT signalling CRC cells. Mechanistically, interaction studies various cell lines vitro binding studies, together with computational modelling, revealed that mediated via unstructured N-terminus requires absence full-length APC....
Abstract The contribution of deubiquitylating enzymes to β-Catenin stabilisation in intestinal stem cells and colorectal cancer (CRC) is poorly understood. Here, we report the deubiquitylase USP10 as an APC-truncation- specific enhancer stability, potentiating WNT signalling CRC. Mechanistically, interaction vitro binding studies, together with computational modelling, revealed that mediated via unstructured N-terminus requires absence full-length APC. Reduction induces expression...