A5084904277- Syphilis Diagnosis and Treatment
- Reproductive tract infections research
- Virology and Viral Diseases
- Antimicrobial Peptides and Activities
- Bacterial Identification and Susceptibility Testing
- Enzyme Structure and Function
- Biochemical and Structural Characterization
- Malaria Research and Control
- Sex work and related issues
- Protein Structure and Dynamics
- Mycobacterium research and diagnosis
- Machine Learning in Bioinformatics
- HIV/AIDS oral health manifestations
- Clostridium difficile and Clostridium perfringens research
- HIV/AIDS Research and Interventions
- Bacterial Infections and Vaccines
- Immunodeficiency and Autoimmune Disorders
- Blood properties and coagulation
- Metabolomics and Mass Spectrometry Studies
- Respiratory viral infections research
- Advanced Proteomics Techniques and Applications
- Redox biology and oxidative stress
- Aquaculture disease management and microbiota
- Leptospirosis research and findings
- HIV/AIDS Impact and Responses
StarRecreation
2025
University of Victoria
2015-2024
Dalhousie University
2021-2024
University of Alberta
2020
Queen's University Belfast
1997-2010
University of Zimbabwe
1987-2007
Structural Genomics Consortium
2006
Hospital for Sick Children
2003
University of Toronto
2003
University College London
1998
Abstract Background Antibiotic resistance is a growing global health concern prompting researchers to seek alternatives conventional antibiotics. Antimicrobial peptides (AMPs) are attracting attention again as therapeutic agents with promising utility in this domain, and using silico methods discover novel AMPs strategy that gaining interest. Such can sift through large volumes of candidate sequences reduce lab screening costs. Results Here we introduce AMPlify, an attentive deep learning...
Iatif, Ahmed S.; Katzenstein, David A.; Bassett, Mary T.; Houston, Stan; Emmanuel, Jean C.; Marowa, Evaristo
Type II fatty acid biosynthesis systems are essential for membrane formation in bacteria, making the constituent proteins of this pathway attractive targets antibacterial drug discovery. The third step elongation cycle type is catalyzed by beta-hydroxyacyl-(acyl carrier protein) (ACP) dehydratase. There two isoforms. FabZ, which catalyzes dehydration (3R)-hydroxyacyl-ACP to trans-2-acyl-ACP, a universally expressed component bacterial system. FabA, second isoform, as has more limited...
Abstract Syphilis is a prominent disease in low- and middle-income countries, re-emerging public health threat high-income countries. elimination will require development of an effective vaccine that has thus far remained elusive. Here we assess the potential Tp0751, vascular adhesin from causative agent syphilis, Treponema pallidum subsp. . Tp0751-immunized animals exhibit significantly reduced bacterial organ burden upon T. challenge compared with unimmunized animals. Introduction lymph...
Previous mass spectrometry (MS)-based global proteomics studies have detected a combined total of 86% all Treponema pallidum proteins under infection conditions (in vivo-grown T. pallidum). Recently, method was developed for the long-term culture in vitro vitro-cultured Herein, we used our previously reported optimized MS-based approach to characterize protein expression profile conditions. These analyses provided proteome coverage 94%, which extends from new 95%. This study provides more...
Comparison of the complete genome sequence Bacteroides fragilis 638R, originally isolated in USA, was made with two previously sequenced strains UK (NCTC 9343) and Japan (YCH46). The presence 10 loci containing genes associated polysaccharide (PS) biosynthesis, each including a putative Wzx flippase Wzy polymerase, confirmed all three strains, despite lack cross-reactivity between NCTC 9343 638R surface PS-specific antibodies by immunolabelling microscopy. Genomic comparisons revealed an...
Pediatric therapists in school-based practice can incorporate exercise promotion through adaptive cycling for children with disabilities who experience high levels of sedentary behavior and low moderate to vigorous activity. The impacts an pilot program were investigated a community-based participatory study. During eight-week intervention, students had goal riding cycles three times week twenty minutes. Using pre-and post-test design, primary outcomes included individualized attainment...
Abstract Protein crystallization is a major bottleneck in protein X‐ray crystallography, the workhorse of most structural proteomics projects. Because principles that govern are too poorly understood to allow them be used strongly predictive sense, common strategy entails screening wide variety solution conditions identify small subset will support crystal nucleation and growth. We tested hypothesis more efficient strategies could formulated by extracting useful patterns correlations from...
Abstract In animals, the transfer of developmental control from maternal RNAs and proteins to zygotically derived products occurs at midblastula transition. This is accompanied by destabilization a subset transcripts. Drosophila, transcript in absence fertilization requires specific cis-acting instability elements. We show here that egg activation necessary sufficient trigger destabilization. have identified 13 maternal-effect lethal loci that, when mutated, result failure degradation. All...
Treponema pallidum, the causative agent of syphilis, is a highly invasive pathogenic spirochete capable attaching to host cells, invading tissue barrier, and undergoing rapid widespread dissemination via circulatory system. The T. pallidum adhesin Tp0751 was previously shown bind laminin, most abundant component basement membrane, suggesting role for this in colonization bacterial dissemination. We hypothesized that similar other pathogens, interaction with coagulation proteins, such as...
Treponema pallidum is a highly invasive pathogen that undergoes rapid dissemination to establish widespread infection. Previous investigations identified the T. adhesin, pallilysin, as an HEXXH-containing metalloprotease autocatalytic cleavage and degrades laminin fibrinogen. In current study we characterized pallilysin's active site, activation requirements, cellular location, fibrin clot degradation capacity through both in vitro assays heterologous treponemal expression studies....
Syphilis is a chronic disease caused by the bacterium Treponema pallidum subsp. pallidum. disseminates widely throughout host and extravasates from vasculature, process that at least partially dependent upon ability of T. to interact with extracellular matrix (ECM) components. Defining molecular basis for interaction between complicated intractability in vitro culturing genetic manipulation. Correspondingly, few proteins have been identified directly Of these, Tp0751 (also known as...
Background The spirochete bacterium Treponema pallidum ssp. is the etiological agent of syphilis, a chronic multistage disease. Little known about global T. proteome, therefore mass spectrometry studies are needed to bring insights into pathogenicity and protein expression profiles during infection. Methodology/Principal Findings To better understand proteome profile infection, we studied DAL-1 strain bacteria isolated from rabbits using complementary techniques, including multidimensional...
Syphilis is a sexually transmitted infection caused by the spirochete bacterium Treponema pallidum subsp. pallidum. The continued incidence of syphilis demonstrates that screening and treatment strategies are not sufficient to curb this infectious disease, there currently no vaccine available. Herein we demonstrate T. adhesin Tp0751 interacts with endothelial cells line lumen human blood vessels through 67-kDa laminin receptor (LamR). Importantly, LamR also for meningitis-causing...
Comprehensive proteome-wide analysis of the syphilis spirochete, Treponema pallidum ssp. pallidum, is technically challenging due to high sample complexity, difficulties with obtaining sufficient quantities bacteria for analysis, and inherent fragility T. cell envelope which further complicates proteomic identification rare outer membrane proteins (OMPs). The main aim present study was gain a deeper understanding global proteome expression profile under infection conditions. This will...
The obligate anaerobe Bacteroides fragilis is a normal resident of the human gastrointestinal tract. clinically derived B. strain NCTC 9343 produces an extensive array extracellular polysaccharides (EPS), including antigenically distinct large, small and micro- capsules. genome encodes multiple gene clusters potentially involved in biosynthesis EPS, eight which are implicated production variable micro-capsule. We have developed rapid robust method for generating marked markerless deletions,...
The mechanisms that facilitate dissemination of the highly invasive spirochaete, Treponema pallidum, are incompletely understood. Previous studies showed treponemal metalloprotease pallilysin (Tp0751) possesses fibrin clot degradation capability, suggesting a role in dissemination. In current study we report characterization functionally linked protein Tp0750. Structural modelling predicts Tp0750 contains von Willebrand factor type A (vWFA) domain, protein-protein interaction domain commonly...
Abstract Antimicrobial peptides (AMPs) exhibit broad-spectrum antimicrobial activity, and have promise as new therapeutic agents. While the adult North American bullfrog ( Rana [ Lithobates ] catesbeiana ) is a prolific source of high-potency AMPs, aquatic tadpole represents relatively untapped for AMP discovery. The recent publication genome transcriptomic resources provides an opportune bridge between known AMPs bioinformatics-based objective present study was to identify novel with...
Syphilis continues to be a major global health threat with 11 million new infections each year, and burden of 36 cases. The causative agent syphilis, Treponema pallidum subspecies pallidum, is highly virulent bacterium, however the molecular mechanisms underlying T. pathogenesis remain definitively identified. This due fact that currently uncultivatable, inherently fragile thus difficult work with, phylogenetically distinct no conventional virulence factor homologs found in other pathogens....
Bacteroides fragilis is a bacterium that resides in the normal human gastro-intestinal tract; however, it also most commonly isolated Gram-negative obligate anaerobe from clinical infections, such as intra-abdominal abscesses, and common cause of anaerobic bacteraemia. Abscess formation important bacterial containment, limiting dissemination infection In this study, we investigated B. binding degradation fibrinogen, major structural component involved fibrin abscess formation. We have shown...
ABSTRACT The spirochete Treponema pallidum subsp. is the causative agent of syphilis, a chronic, sexually transmitted infection characterized by multiple symptomatic and asymptomatic stages. Although several other species in genus are able to cause or contribute disease, T. differs that it rapidly disseminate via bloodstream tissue sites distant from site initial infection. It also only cross both blood-brain placental barriers. Previously, proteins, Tp0750 Tp0751 (also called pallilysin),...
Syphilis is a chronic, multi-stage infection caused by the extracellular bacterium Treponema pallidum ssp. pallidum. widely disseminates through vasculature, crosses endothelial, blood-brain and placental barriers, establishes systemic infection. Although capacity of T. to traverse endothelium well-described, response endothelial cells exposure, contribution this treponemal traversal, poorly understood.To address knowledge gap, we used quantitative proteomics cytokine profiling characterize...
Journal Article Parainfluenza Virus Respiratory Infection After Heart Transplantation: Successful Treatment with Ribavirin Get access L. Cobian, Cobian Division of Infectious and Tropical Diseases, College Medicine, University South Florida, Tampa, Florida Reprints or correspondence: Dr. Ledya MDC Box 19, 12901 Bruce B. Downs Boulevard, 33612-4799. Search for other works by this author on: Oxford Academic PubMed Google Scholar S. Houston, Houston J. Greene, Greene T. Sinnott Clinical Volume...
Treponema pallidum subspecies is the causative agent of syphilis, a chronic, multistage, systemic infection that remains major global health concern. The molecular mechanisms underlying T. pathogenesis are incompletely understood, partially due to phylogenetic divergence pallidum. One aspect differentiates it from conventional Gram-negative bacteria, and believed play an important role in pathogenesis, its unusual cell envelope ultrastructure; particular, peptidoglycan layer chemically...