A5079581845- Immune Response and Inflammation
- Cytokine Signaling Pathways and Interactions
- Cervical Cancer and HPV Research
- Reproductive tract infections research
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Syphilis Diagnosis and Treatment
- NF-κB Signaling Pathways
- Meat and Animal Product Quality
- HIV Research and Treatment
- interferon and immune responses
- Toxin Mechanisms and Immunotoxins
- Immune Cell Function and Interaction
- Mosquito-borne diseases and control
- Adenosine and Purinergic Signaling
- Phytochemicals and Medicinal Plants
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cell Adhesion Molecules Research
- Animal Behavior and Welfare Studies
- Glycosylation and Glycoproteins Research
- Estrogen and related hormone effects
- Cassava research and cyanide
- Advanced Proteomics Techniques and Applications
- Urological Disorders and Treatments
- Protein purification and stability
University of Antwerp
2012-2024
VIB-UAntwerp Center for Molecular Neurology
2011-2019
Ghent University
1988-2015
Vlaams Instituut voor Biotechnologie
1999-2000
South Australia Pathology
1994-1997
Hanson Institute
1996-1997
University of Edinburgh
1997
Roche (Belgium)
1989
Cervical-vaginal fluid (CVF) plays an important role in the prevention of gynecological infections, although little is known about contribution CVF proteins to immunity lower female genital tract. In order analyze protein composition human CVF, we used samples that are routinely collected during colposcopy, but usually discarded. Since these available large quantities aimed their usefulness for proteomics experiments. The were analyzed using different prefractionation techniques...
Abstract To understand the growth response to drought, we performed a proteomics study in leaf zone of maize ( Zea mays L.) seedlings and functionally characterized role starch biosynthesis regulation growth, photosynthesis antioxidant capacity, using shrunken‐2 mutant sh2 ), defective ADP‐glucose pyrophosphorylase. Drought altered abundance 284 proteins overrepresented for photosynthesis, amino acid, sugar metabolism, redox‐regulation. Changes protein levels correlated with enzyme...
Cervicovaginal fluid has an important function in the homeostasis and immunity of lower female genital tract. Analysis cervicovaginal proteome may therefore yield information about pathogenesis numerous gynecological pathologies. Additionally, great potential as a source biomarkers for these conditions.This review provides detailed discussion human proteomics studies performed to characterize this biological fluid. Furthermore, infection-correlated pathological conditions tract are discussed...
Objectives Cervicovaginal fluid (CVF) can be considered as a potential source of biomarkers for diseases the lower female reproductive tract. The easily collected, thereby offering new opportunities such development self tests. Our objective was to identify CVF protein biomarker cervical cancer or its precancerous state. Methods A differential proteomics study set up using samples from healthy and women. Label-free spectral counting applied quantify abundances. Results proteome analysis...
The performance and acceptability of first-void urine as specimen for the detection HPV DNA in a Belgian referral population was evaluated using an optimized sample collection processing protocol. One hundred ten cervical samples were collected from 25- to 64-year-old women who referred colposcopy (January–November 2016). Paired analyzed by Riatol qPCR genotyping assay. Acceptability data gathered through questionnaires (NCT02714127). A higher high-risk prevalence observed (n = 76/110)...
Background The spirochete bacterium Treponema pallidum ssp. is the etiological agent of syphilis, a chronic multistage disease. Little known about global T. proteome, therefore mass spectrometry studies are needed to bring insights into pathogenicity and protein expression profiles during infection. Methodology/Principal Findings To better understand proteome profile infection, we studied DAL-1 strain bacteria isolated from rabbits using complementary techniques, including multidimensional...
Chikungunya virus (CHIKV) is an arbovirus with a global spread and significant public health impact. It positive stranded RNA alphavirus belonging to the Togaviridae family. However, many questions about replication cycle of CHIKV remain unanswered. The entry process not completely understood nor are associated virus-receptor interactions fully identified. Here, we designed affinity purification mass spectrometry coupled approach that allowed identification factors facilitate in human cells....
We examined the interaction of ECM1 (extracellular matrix protein 1) using yeast two-hybrid screening and identified type II transmembrane protein, PLSCR1 (phospholipid scramblase 1), as a binding partner. This was then confirmed by in vitro vivo co-immunoprecipitation experiments, additional pull-down experiments with GST-tagged ECM1a fragments localized this to occur within tandem repeat region ECM1a. Furthermore, immunohistochemical staining revealed partial overlap human skin at basal...
Background HIV-1 replication depends on a delicate balance between cellular co-factors and antiviral restriction factors. Lens epithelium-derived growth factor (LEDGF/p75) benefits HIV, whereas apolipoprotein B mRNA-editing catalytic polypeptide-like 3G (APOBEC3G), tripartite motif 5alpha (TRIM5α), tetherin exert anti-HIV activity. Expression levels of these proteins possibly contribute to resistance in HIV-1-exposed populations. Methodology/Principal Findings We used real-time PCR flow...
Previously, we reported that the cytotoxic activity of human (h) tumor necrosis factor (TNF) on murine (m) L929 cells requires integrity three loops (positions 30-36, 84-88 and 138-150) which cluster around interface between each two subunits trimeric hTNF structure. The collection mutants was further characterized by their various cell systems as well binding to types receptor (R), R55 R75. It turned out amino acids (Leu29 Arg32) were specifically involved in hR75 binding, Leu29-->Ser...
TNF, a cytokine with cytotoxic activity on variety of tumor cells, is mainly produced by macrophages; however, some cell types non-macrophage origin, apparently resistant to TNF-mediated lysis, can also produce TNF. It not clear whether these cells were TNF-resistant priori or protective mechanisms against toxicity autocrine TNF may be induced in TNF-producing cells. Murine L929sA fibrosarcoma which are highly sensitive cytotoxicity, transfected the neomycin resistance (neor) gene, alone...