A5091427018- HIV Research and Treatment
- Cystic Fibrosis Research Advances
- HIV/AIDS Research and Interventions
- Cancer, Hypoxia, and Metabolism
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Cancer, Lipids, and Metabolism
- Blood groups and transfusion
- Respiratory viral infections research
- Eicosanoids and Hypertension Pharmacology
- Immune Cell Function and Interaction
- Cytomegalovirus and herpesvirus research
- Inhalation and Respiratory Drug Delivery
- Cardiac Ischemia and Reperfusion
- Caveolin-1 and cellular processes
- COVID-19 Impact on Reproduction
- Nitric Oxide and Endothelin Effects
- Diagnosis and treatment of tuberculosis
- Immunodeficiency and Autoimmune Disorders
- Inflammatory mediators and NSAID effects
- Cardiac tumors and thrombi
- Mitochondrial Function and Pathology
- Pneumocystis jirovecii pneumonia detection and treatment
- HIV-related health complications and treatments
- Bacterial biofilms and quorum sensing
Sciensano (Belgium)
2019-2024
UCLouvain
2003-2023
Cystic Fibrosis Trust
2021
Research Institute for Tropical Medicine
2019
Instituut voor Tropische Geneeskunde
2011-2017
Haute École Francisco Ferrer
2017
Cheikh Anta Diop University
2012
Ludwig-Maximilians-Universität München
2004
Nitric oxide (NO) is a powerful angiogenic mediator acting downstream of vascular endothelial growth factor (VEGF). Both the NO synthase (eNOS) and VEGFR-2 receptor colocalize in caveolae. Because structural protein these signaling platforms, caveolin, also represses eNOS activity, changes its abundance are likely to influence process various ways. In this study, we used mice deficient for caveolin-1 gene (Cav −/− ) examine impact caveolae suppression model adaptive angiogenesis obtained...
The therapeutic effects of nonspecific beta-blockers are limited by vasoconstriction, thus justifying the interest in molecules with ancillary vasodilating properties. Nebivolol is a selective beta1-adrenoreceptor antagonist that releases nitric oxide (NO) through incompletely characterized mechanisms. We identified endothelial beta3-adrenoreceptors human coronary microarteries mediate endothelium- and NO-dependent relaxation hypothesized nebivolol activates these...
The interaction of the heat shock protein 90 (Hsp90) with endothelial NO synthase (eNOS) has been shown to account for a sustained production in vitro. Here, we examined whether overexpression Hsp90 pig model cardiac infarct could preserve myocardium from deleterious effects ischemia-reperfusion.Percutaneous liposome-based gene transfer was performed by retroinfusion anterior interventricular vein before left descending occlusion and reperfusion. We found that recombinant expression ischemic...
Background CD4+ T-cell enumeration (CD4 count) is used as a criterion to initiate antiretroviral therapy (ART) in HIV patients and monitor treatment efficacy. However, simple, affordable, reliable point-of-care (POC) instruments adapted resource-limited settings are still lacking. The PIMA CD4 analyzer new POC instrument for counting that uses disposable cartridges battery-powered analyzer. Methods Whole blood samples were taken by venipuncture or finger prick from 300 subjects, including...
Abstract Purpose: Cyclic hypoxia in tumors originates from heterogeneities RBC flux and influences not only tumor cells but also endothelial lining blood vessels. Whether pO2 fluctuations, particularly transient reoxygenation periods, alter the well-known hypoxia-inducible factor (HIF)–dependent gene program is largely unknown. Experimental Design: We compared transcriptomic profiles of exposed to cyclic versus continuous uncover a possible differential effect on angiogenesis metastases....
CD4⁺ T-cell counts are used to screen and follow-up HIV-infected patients during treatment. As part of the World Health Organization prequalification program diagnostics, we conducted an independent multicenter evaluation FACSCount CD4 Pima CD4, using FACSCalibur as reference method.A total 440 paired capillary venous blood samples were collected from attending HIV outpatient clinic in Antwerp, Belgium, care treatment center Dar es Salam, Tanzania. Capillary was run on analyzer, whereas...
Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in people with cystic fibrosis (pwCF) can lead to severe outcomes. Methods In this observational study, the European Cystic Fibrosis Society Patient Registry collected data on pwCF and SARS-CoV-2 estimate incidence, describe clinical presentation investigate factors associated outcomes using multivariable analysis. Results Up December 31, 2020, 26 countries reported information 828 infection. Incidence was 17.2...
Heterogeneities in tumor blood flow are associated with cyclic changes pO2 or hypoxia. A major difference from O2 diffusion-limited chronic hypoxia is that the vasculature itself may be directly influenced by fluctuating hypoxic environment, and reoxygenation phases complicate usual hypoxia-induced phenotypic pattern. Here, we determined hypoxia-driven pathways modulate inducible factor (HIF)-1alpha abundance endothelial cells to identify possible therapeutic targets. We found exposure of...
Background HIV-1 replication depends on a delicate balance between cellular co-factors and antiviral restriction factors. Lens epithelium-derived growth factor (LEDGF/p75) benefits HIV, whereas apolipoprotein B mRNA-editing catalytic polypeptide-like 3G (APOBEC3G), tripartite motif 5alpha (TRIM5α), tetherin exert anti-HIV activity. Expression levels of these proteins possibly contribute to resistance in HIV-1-exposed populations. Methodology/Principal Findings We used real-time PCR flow...
Introduction Point-of-care (POC) CD4 testing can improve access to treatment by enabling decentralization and reducing patient loss-to-follow-up. As new POC technologies become available, their performance should be assessed before widespread deployment. This study reports the findings of five independent evaluations PointCare NOW system. Materials/Methods Evaluations were conducted in Southern Africa (Mozambique, South Africa) North America (Canada, USA). 492 blood samples (55 from...
Cyclosporine A (CsA) is associated with the development of cardiovascular toxicity in transplant patients but can exert myocardial protection against ischemia/reperfusion damages. We examined a rat model chronic CsA administration whether subtle variations NO pathway could account for these opposite effects. treatment rapidly led to an increase Hsp90 expression promoting recruitment Akt and calcineurin, thereby eNOS activation through Ser1177 phosphorylation Thr495 dephosphorylation,...
Background Flow Cytometry (FCM) is still considered to be the method of choice for accurate CD4 enumeration. However, use FCM in developing countries problematic due their cost and complexity. Lower-cost technologies have been introduced. We evaluated CyFlow Counter together with its lyophilized reagents, Pima high-temperature area, using FACSCount as reference. Materials Methods Whole blood samples were consecutively collected by venipuncture from 111 HIV+ patients 17 HIV-negative donors....
Emergence of Multidrug-resistant (MDR) strains constitutes a significant public health problem worldwide. Prevalence MDR tuberculosis from Chad is unavailable to date.We collected samples consecutive TB patients nationwide in the seven major cities between 2007 and 2012 characterize drug resistance population structure circulating Mycobacterium complex (MTBC) strains. We tested sensitivity using Line Probe Assays phenotypic susceptibility testing (DST) were used for second line drugs....
Background CD4 T-cell counts are widely used to assess treatment eligibility and follow-up HIV-infected patients. The World Health Organization prequalification of in vitro diagnostics program conducted a performance evaluation the FACSPresto (BD Biosciences), new point-of-care instrument measure absolute CD4-T cell (CD4) percentages venous capillary blood samples from Methods Patients were recruited Belgium (200 patients) Tanzania (247 patients). Venous analyzed two nearby reference...
Besides inclusion in 1st line regimens against tuberculosis (TB), pyrazinamide (PZA) is used 2nd anti-TB regimens, including the short regimen for multidrug-resistant TB (MDR-TB) patients. Guidelines and expert opinions are contradictory about of PZA case resistance. Moreover, drug susceptibility testing (DST) not often applied routine testing, prevalence resistance unknown several regions, most African countries.Six hundred twenty-three culture isolates from rifampicin-resistant (RR)...
Aim To examine the trajectory of forced expiratory volume in 1 s (FEV ) using data from European Cystic Fibrosis Society patient registry (ECFPR) collected 2008 to 2016, i.e. era before highly effective modulator therapy (HEMT). We evaluated risk factors for FEV decline. Methods The study population included patients with a confirmed diagnosis cystic fibrosis recorded ECFPR (2008–2016). evolution % predicted (%FEV age, and yearly change %FEV were evaluated. Risk considered transmembrane...
Factors associated with severe COVID-19 infection have been identified; however, the impact of on longer-term outcomes is unclear. The objective this study was to examine trajectory lung function and nutritional status in people cystic fibrosis (pwCF).
HIV-infected patients require antiretroviral treatment for life. To improve access to care, CD4 enumeration and viral load tests have been redesigned be used as point-of-care techniques using finger-stick blood. Accurate counting in capillary blood requires a free flowing drop that is achieved by blade incision. The aim of this study was assess the attitude toward blade-based donation.Four hundred ninety-nine were included (299 from South Africa 200 Belgium). They completed questionnaire...
CD4 counts are currently used to assess HIV patients for treatment eligibility and monitor antiretroviral response treatment. The emerging point-of-care devices could fill an important gap in resource-limited settings. However, the accuracy of CD4-counting instruments is diverse data on how measurement errors have impact clinical decisions lacking.Clinicians were queried use results their setting. Subsequently, effect initiation was put a statistical model. Based databases from Belgium,...
Pyrazinamide (PZA) is an antibacterial used in the first-line regimen against tuberculosis (TB) for its action dormant bacilli. PZA also included new short to treat multidrug-resistant TB (MDR-TB). However, prevalence and significance of resistance not known Central West Africa.Between 2013 2016, we collected samples from MDR-TB patients recruited observational study implementing six countries: Burundi (n=35), Cameroon (n=135), Niger (n=57), African Republic Democratic Congo (n=99), Rwanda...