Optical theranostic nanoagents that seamlessly and synergistically integrate light-generated signals with photothermal or photodynamic therapy can provide opportunities for cost-effective precision medicine, while the potential clinical translation requires them to have good biocompatibility high imaging/therapy performance. We herein report an intraparticle molecular orbital engineering approach simultaneously enhance photoacoustic brightness efficacy of semiconducting polymer nanoparticles...

Optogenetics provides powerful means for precise control of neuronal activity; however, the requirement transgenesis and incapability to extend neuron excitation window into deep-tissue-penetrating near-infrared (NIR) region partially limit its application. We herein report a potential alternative approach optogenetics using semiconducting polymer nanobioconjugates (SPNsbc) as photothermal nanomodulator thermosensitive ion channels in neurons. SPNsbc are designed efficiently absorb NIR light...

Photoacoustic (PA) imaging holds great promise for preclinical research and clinical practice. However, most studies rely on the laser wavelength in first near-infrared (NIR) window (NIR-I, 650–950 nm), while few have been exploited second NIR (NIR-II, 1000–1700 mainly due to lack of NIR-II absorbing contrast agents. We herein report synthesis a broadband PA agent based semiconducting polymer nanoparticles (SPN-II) apply it window. SPN-II can absorb both NIR-I regions, providing feasibility...

Phototheranostic nanoagents are promising for early diagnosis and precision therapy of cancer. However, their imaging ability therapeutic efficacy often limited due to the presence delivery barriers in tumor microenvironment. Herein, we report development organic multimodal phototheranostic that can biomimetically target cancer-associated fibroblasts microenvironment enhanced imaging-guided cancer therapy. Such biomimetic nanocamouflages comprise a near-infrared (NIR) absorbing...

Theranostic nanoagents are promising for precision medicine. However, biodegradable with the ability photoacoustic (PA) imaging guided photothermal therapy (PTT) rare. We herein report development of semiconducting polymer nanoparticles (SPNs) enhanced PA and PTT efficacy cancer therapy. The design capitalizes on enzymatically oxidizable nature vinylene bonds in conjunction chemistry to synthesize a (DPPV) transform it into water-soluble (SPNV). As compared its counterpart SPN (SPNT),...

An activatable photoacoustic nanoprobe based on a semiconducting oligomer with amplified brightness and pH-sensing capability is developed by taking advantage of nanodoping to simultaneously create both intraparticle photoinduced electron transfer intramolecular protonation within single particle. This organic permits noninvasive real-time ratiometric imaging pH in tumors living mice through systemic administration at relatively low dosage. As service our authors readers, this journal...

Therapeutic enzymes hold great promise for cancer therapy; however, in vivo remote control of enzymatic activity to improve their therapeutic specificity remains challenging. This study reports the development an organic semiconducting pro-nanoenzyme (OSPE) with a photoactivatable feature metastasis-inhibited therapy. Upon near-infrared (NIR) light irradiation, this not only generates cytotoxic singlet oxygen (1O2) photodynamic therapy (PDT), but also triggers spontaneous cascade reaction...

Real-time imaging of immunoactivation is imperative for cancer immunotherapy and drug discovery; however, most existing agents possess "always-on" signals thus have poor signal correlation with immune responses. Herein, renal-clearable near-infrared (NIR) fluorescent macromolecular reporters are synthesized to specifically detect an immunoactivation-related biomarker (granzyme B) real-time evaluation immunotherapy. Composed a peptide-caged NIR signaling moiety linked hydrophilic...

Abstract Photoacoustic (PA) imaging and photothermal therapy (PTT) provide useful tools for diagnosis treatments of diseases. However, current applications PA PTT are greatly limited by their relying on light source in the first near‐infrared (NIR) window (NIR‐I, 750–1000 nm), which shows shallow tissue penetration depth. Recently, this predicament is addressed use second NIR (NIR‐II) ranging from 1000 to 1700 nm due reduced light–tissue interaction increased allowable power density...

The detection of exosomes is promising for the early diagnosis cancer. However, development suitable optical sensors remains challenging. We have developed first luminescent nanosensor multiplex differentiation cancer that bypasses real-time light excitation. sensor composed a near-infrared semiconducting polyelectrolyte (ASPN) forms complex with quencher-tagged aptamer. afterglow signal nanocomplex (ASPNC), being initially quenched, turned on in presence aptamer-targeted exosomes. Because...

Despite its great potential in cancer treatment, photodynamic therapy (PDT) often exacerbates hypoxia and subsequently compromises therapeutic efficacy. To overcome this issue, an organic nanoinhibitor (OPNi) has been synthesized that the additional ability to counteract carbonic anhydrase IX (CA-IX), a molecular target hypoxia-mediated signalling cascade. OPNi is composed of metabolizable semiconducting polymer as photosensitizer CA-IX antagonist conjugated amphiphilic matrix. This...

Abstract Detection of metastatic tumor tissues is crucial for cancer therapy; however, fluorescence agents that allow to do share the disadvantage low signal‐to‐background ratio due tissue autofluorescence. The development amphiphilic poly( p ‐phenylenevinylene) derivatives can self‐assemble into nanoagent (SPPVN) in biological solutions and emit near‐infrared afterglow luminescence after cessation light irradiation ultrasensitive imaging tumors living mice herein reported. As compared with...

Abstract Regulation of transgene systems is needed to develop innovative medicines. However, noninvasive remote control gene expression has been rarely developed and remains challenging. We herein synthesize a near‐infrared (NIR) absorbing dendronized semiconducting polymer (DSP) utilize it as photothermal nanocarrier not only efficiently deliver genes but also spatiotemporally in conjunction with heat‐inducible promoter. DSP high conversion efficiency (44.2 %) at 808 nm, permitting fast...

Abstract Smart molecular probes that emit deep‐tissue penetrating photoacoustic (PA) signals responsive to the target of interest are imperative understand disease pathology and develop innovative therapeutics. This study reports a self‐assembly approach semiconducting macromolecular activatable probe for in vivo imaging reactive oxygen species (ROS). comprises near‐infrared absorbing phthalocyanine core four poly(ethylene glycol) (PEG) arms linked by ROS‐responsive self‐immolative segments....

Protein sulfenic acids play a key role in oxidative signal transduction of many biological and pathological processes; however, current chemical tools rely on visible fluorescence signals, limiting their utility to vitro assays. We herein report reaction-based semiconducting polymer nanoprobes (rSPNs) with near-infrared absorption for vivo photoacoustic (PA) imaging protein acids. rSPNs comprise an optically active as the core shielded inert silica poly(ethylene glycol) corona. The acid...

Despite its high morbidity and mortality, contrast-induced acute kidney injury (CIAKI) remains a diagnostic dilemma because it relies on in vitro detection of insensitive late-stage blood urinary biomarkers. We report the synthesis an activatable duplex reporter (ADR) for real-time vivo imaging CIAKI. ADR is equipped with chemiluminescence near-infrared fluorescence (NIRF) signaling channels that can be activated by oxidative stress (superoxide anion, O2.- ) lysosomal damage...

Noninvasive regulation of CRISPR/Cas9 gene editing is conducive to understanding function and development therapy; however, it remains challenging. Herein, a photolabile semiconducting polymer nanotransducer (pSPN) synthesized act as the vector deliver plasmids into cells also photoregulator remotely activate editing. pSPN comprises 1 O2 -generating backbone grafted with polyethylenimine brushes through -cleavable linkers. NIR photoirradiation spontaneously triggers cleavage vectors from...

Semiconducting polymer nanoparticles (SPNs) have emerged as an alternative class of optical nanoagents for imaging applications. However, the general preparation method SPNs is nanoprecipitation, which likely to encounter issue nanoparticle dissociation. We herein report nondissociable near-infrared (NIR)-absorbing organic semiconducting in vivo photoacoustic (PA) and fluorescence imaging. The are self-assembled from amphiphilic oligomer (ASO) that has a hydrophobic backbone attached by...

All-carbon LIC with higher energy density and lower cost are constructed based on NaHCO₃ crystal designed N-doped carbon network.